A5018163868- Liver Disease Diagnosis and Treatment
- RNA Research and Splicing
- Single-cell and spatial transcriptomics
- Lipid metabolism and disorders
- Liver physiology and pathology
- Trace Elements in Health
- Acupuncture Treatment Research Studies
- S100 Proteins and Annexins
- interferon and immune responses
- Metabolism, Diabetes, and Cancer
- Biochemical effects in animals
- Digestive system and related health
- Immune Response and Inflammation
- Pediatric Pain Management Techniques
- Dental Anxiety and Anesthesia Techniques
- RNA modifications and cancer
- Pancreatic function and diabetes
- Ion channel regulation and function
- Genetics and Neurodevelopmental Disorders
- Drug Transport and Resistance Mechanisms
- Endoplasmic Reticulum Stress and Disease
- Pain Mechanisms and Treatments
Helmholtz Zentrum München
2020-2024
University of Ioannina
2010-2018
The bone marrow in the skull is important for shaping immune responses brain and meninges, but its molecular makeup among bones relevance human diseases remain unclear. Here, we show that mouse has most distinct transcriptomic profile compared with other states of health injury, characterized by a late-stage neutrophil phenotype. In humans, proteome analysis reveals distinct, differentially expressed neutrophil-related pathways unique synaptic protein signature. 3D imaging demonstrates...
Abstract Single-cell RNA-seq reveals the role of pathogenic cell populations in development and progression chronic diseases. In order to expand our knowledge on cellular heterogeneity, we have developed a single-nucleus RNA-seq2 method tailored for comprehensive analysis nuclear transcriptome from frozen tissues, allowing dissection all types present liver, regardless size or fragility. We use this approach characterize transcriptional profile individual hepatocytes with different levels...
Background & Aims Polyploidy in hepatocytes has been proposed as a genetic mechanism to buffer against transcriptional dysregulation. Here, we aim demonstrate the role of polyploidy modulating gene regulatory networks during ageing. Methods We performed single-nucleus RNA-sequencing hepatocyte nuclei different ploidy levels isolated from young and old wild-type mice. Changes expression network were compared three independent haploinsufficient strains for HNF4A, CEBPA or CTCF, representing...
Fibrotic organ responses have recently been identified as long-term complications in diabetes. Indeed, insulin resistance and aberrant hepatic lipid accumulation represent driving features of progressive non-alcoholic fatty liver disease (NAFLD), ranging from simple steatosis steatohepatitis (NASH) to fibrosis. Effective pharmacological regimens stop are still lacking to-date.Based on our previous discovery transforming growth factor beta-like stimulated clone (TSC)22D4 a key driver glucose...
Abstract The histone methylase Setdb1 plays a pivotal role in embryonic stem cell maintenance and developmental lineage specification. However, its function adult cells remains elusive. Here we show that conditional inactivation of Lgr5 + intestinal alters the transcriptional programs progeny types results increased cell-to-cell variability. Loss blocked differentiation towards absorptive enterocyte lineage, while secretory were only marginally affected due to activation alternative...
Abstract Single-cell RNA-seq reveals the role of pathogenic cell populations in development and progression chronic diseases. In order to expand our knowledge on cellular heterogeneity we have developed a single-nucleus RNA-seq2 method that allows deep characterization nuclei isolated from frozen archived tissues. We used this approach characterize transcriptional profile individual hepatocytes with different levels ploidy, discovered gene expression tetraploid mononucleated is conditioned...
ABSTRACT Objective Fibrotic organ responses have recently been identified as long-term complication in diabetes. Indeed, insulin resistance and aberrant hepatic lipid accumulation represent driving features of progressive non-alcoholic fatty liver disease (NAFLD), ranging from simple steatosis steatohepatitis (NASH) to fibrosis. Effective pharmacological regimens stop are still lacking to-date. Methods Based on our previous discovery transforming growth factor beta-like stimulated clone...