A5041604591- Traumatic Brain Injury and Neurovascular Disturbances
- Electron and X-Ray Spectroscopy Techniques
- Nanoparticle-Based Drug Delivery
- Advanced Electron Microscopy Techniques and Applications
- Barrier Structure and Function Studies
- Neuroinflammation and Neurodegeneration Mechanisms
- Molecular Biology Techniques and Applications
- Cell Image Analysis Techniques
- S100 Proteins and Annexins
- Advanced biosensing and bioanalysis techniques
- Molecular Sensors and Ion Detection
- Nerve injury and regeneration
- Lipid Membrane Structure and Behavior
- Nanoplatforms for cancer theranostics
- Luminescence and Fluorescent Materials
- Single-cell and spatial transcriptomics
- Quantum Dots Synthesis And Properties
- Neuroscience of respiration and sleep
- Neuroscience and Neuropharmacology Research
- Ion Transport and Channel Regulation
- Neurogenesis and neuroplasticity mechanisms
- Acute Ischemic Stroke Management
- Cardiac Arrest and Resuscitation
- Traumatic Brain Injury Research
- Neurological Disorders and Treatments
Munich Cluster for Systems Neurology
2020-2025
Inserm
2023-2025
Université de Caen Normandie
2023-2025
Normandie Université
2023-2025
Cyceron
2023-2025
Physiopathologie et imagerie des troubles neurologiques
2023-2025
Ludwig-Maximilians-Universität München
2021-2025
LMU Klinikum
2021-2025
Universitätsmedizin Greifswald
2024
Nihon University
2024
The bone marrow in the skull is important for shaping immune responses brain and meninges, but its molecular makeup among bones relevance human diseases remain unclear. Here, we show that mouse has most distinct transcriptomic profile compared with other states of health injury, characterized by a late-stage neutrophil phenotype. In humans, proteome analysis reveals distinct, differentially expressed neutrophil-related pathways unique synaptic protein signature. 3D imaging demonstrates...
Efficient and accurate nanocarrier development for targeted drug delivery is hindered by a lack of methods to analyze its cell-level biodistribution across whole organisms. Here we present Single Cell Precision Nanocarrier Identification (SCP-Nano), an integrated experimental deep learning pipeline comprehensively quantify the targeting nanocarriers throughout mouse body at single-cell resolution. SCP-Nano reveals tissue distribution patterns lipid nanoparticles (LNPs) after different...
Currently, traumatic brain injury (TBI) is the leading cause of death or disabilities in young individuals worldwide. The multi-complexity its pathogenesis as well impermeability blood–brain barrier (BBB) makes drug choice and delivery very challenging. brain-derived neurotrophic factor (BDNF) regulates neuronal plasticity, cell growth, proliferation, survival long-term memory. However, short half-life low BBB permeability are main hurdles to be an effective therapeutic for TBI. Poly...
Visualizing single organic nanoparticles (NPs) in vivo remains a challenge, which could greatly improve our understanding of the bottlenecks field nanomedicine. To achieve high single-particle fluorescence brightness, we loaded polymer poly(methyl methacrylate)-sulfonate (PMMA-SO3H) NPs with octadecyl rhodamine B together bulky hydrophobic counterion (perfluorinated tetraphenylborate) as fluorophore insulator to prevent aggregation-caused quenching. create stealth properties, used...
Traumatic brain injury (TBI) causes acute and subacute tissue damage, but is also associated with chronic inflammation progressive loss of months years after the initial event. The trigger subsequent molecular mechanisms causing TBI are not well understood. aim current study was therefore to investigate hypothesis that necroptosis, a form programmed cell death mediated by interaction Receptor Interacting Protein Kinases (RIPK) 1 3, involved in this process. Neuron-specific RIPK1- or...
Abstract The current lack of understanding about how nanocarriers cross the blood‐brain barrier (BBB) in healthy and injured brain is hindering clinical translation nanoscale brain‐targeted drug‐delivery systems. Here, bio‐distribution lipid nano‐emulsion droplets (LNDs) two sizes (30 80 nm) mouse after traumatic injury (TBI) investigated. highly fluorescent LNDs are prepared by loading them with octadecyl rhodamine B a bulky hydrophobic counter‐ion, tetraphenylborate. Using vivo two‐photon...
Subarachnoid hemorrhage (SAH) is characterized by acute and delayed reductions of cerebral blood flow (CBF) caused, among others, spasms arteries arterioles. Recently, the inactivation perivascular macrophages (PVM) has been demonstrated to improve neurological outcomes after experimental SAH, but underlying mechanisms protection remain unclear. The aim our exploratory study was, therefore, investigate role PVM in formation microvasospasms SAH.PVMs were depleted 8- 10-week-old male C57BL/6...
Molecular probes for cell plasma membranes are indispensable fluorescence imaging. Herein, we present an array of five anionic cyanine-based turn-on membrane with emission spanning from green to near infrared. They analogous the commonly used MemBright probe family, where two zwitterionic anchor groups replaced sulfonates dodecyl chains. The developed provide selective wash-free staining live cells in vitro, featuring improved brightness and slower internalization inside cells. In comparison...
Traumatic brain injury (TBI) is a leading cause of death and disability in individuals worldwide. Producing clinically relevant TBI model small-sized animals remains fairly challenging. For good screening potential therapeutics, which are effective the treatment TBI, animal models should be established standardized. In this study, we mouse closed head using Shohami weight-drop method with some modifications concerning cognitive deficiency assessment provided detailed description severe...
Abstract Incomplete reperfusion of the microvasculature (‘no-reflow’) after ischaemic stroke damages salvageable brain tissue. Previous ex vivo studies suggest pericytes are vulnerable to ischaemia and may exacerbate no-reflow, but viability their association with no-reflow remains under-explored in vivo. Using longitudinal two-photon single-cell imaging over 7 days, we showed that 87% constrict during cerebral remain constricted post reperfusion, 50% pericyte population acutely damaged....
Mild traumatic brain injury (mTBI) accounts for 80% of all TBI, may be associated with chronic impairments, and is difficult to diagnose due a lack objective markers. In this study, we investigated whether neurosteroids can serve as blood biomarkers mTBI. Two cohorts C57BL/6 mice were subjected model mTBI combining impact rotational acceleration or sham surgery. The first cohort underwent neurological testing anxiety, balance, locomotion before after For the second cohort, brains plasma...
Nanotechnology holds great promise for improving the delivery of therapeutics to brain. However, current approaches often operate at organ or tissue level and are limited by lack tools dynamically monitor cargo in vivo. We have developed highly fluorescent lipid nanodroplets (LNDs) that enable tracking nanocarrier behavior subcellular while also carrying a Förster resonance energy transfer (FRET)-based drug detection system (FedEcs) capable monitoring release Using two-photon microscopy, we...
Nanoparticles have a great potential to significantly improve the delivery of therapeutics brain and may also be equipped with properties investigate function. The brain, being highly complex organ shielded by selective barriers, requires its own specialized detection system. However, significant hurdle achieve these goals is still identification individual nanoparticles within sufficient cellular, subcellular, temporal resolution. This review aims provide comprehensive summary current...
Abstract Triggering receptor expressed on myeloid cells 2 (TREM2) is a central regulator of microglial activity and sequence variants are major risk factors for late onset Alzheimer’s disease (LOAD). To better understand the molecular functional changes associated with TREM2 signalling, we generated reporter mouse model observed gradual upregulation expression increasing plaque proximity. Isolated microglia were sorted based their transcriptomic profiles acquired in both wildtype APP...
SUMMARY The meninges of the brain are an important component neuroinflammatory response. Diverse immune cells move from calvaria marrow into dura mater via recently discovered skull-meninges connections (SMCs). However, how bone is different other bones and whether it contributes to human diseases remain unknown. Using multi-omics approaches whole mouse transparency we reveal that highly heterogeneous across body. harbors most distinct molecular signature with hundreds differentially...
Like other volume electron microscopy approaches, Automated Tape Collecting Ultramicrotomy (ATUM) enables imaging of serial sections deposited on thick plastic tapes by scanning (SEM). However, ATUM is unique enabling hierarchical and thus efficient screening for target structures as needed e.g., correlated light microscopy. SEM tape can only access the section surface, thereby limiting axial resolution to typical size cellular vesicles, an order magnitude lower than acquired xy resolution....
The increase in the incidence of radiation-induced skin injury cases and absence standard treatments escalate interest finding new effective drugs for these lesions. We studied effect a 40% solution arginine glutamate on healing ulcers guinea pigs.Radiation was produced thigh pigs by 60 Gy local X-ray irradiation. Treatment started 6 weeks after irradiation when had been formed. Arginine administered subcutaneous injections around wound edge. Methyluracil chosen as comparison drug. animals...
Traumatic brain injury (TBI) causes long-lasting neurodegeneration and cognitive impairments; however, the underlying mechanisms of these processes are not fully understood. Acid-sensing ion channels 1a (ASIC1a) voltage-gated Na+- Ca2+-channels shown to be involved in neuronal cell death; their role for chronic post-traumatic damage is largely unknown. To address this issue, we used ASIC1a-deficient mice investigated outcome up 6 months after TBI. wild-type (WT) littermates were subjected...
Background : Increased multidrug resistance of extended-spectrum beta-lactamases (ESBLs) compromises the efficacy treatment urinary tract infections. Objective The objective this study is to determine prevalence ESBL-producing uropathogens from hospitalized patients with chronic pyelonephritis and identify presence genes involved in resistance. Methods A cross-sectional 105 pyelonephritis, treated Kharkiv City Clinical Emergency Hospital, Ukraine was carried. Bacterial isolates were...
ABSTRACT Targeting nanoparticle therapeutics with cellular accuracy in whole organisms could open breakthrough opportunities precision medicine. However, evaluating and fine-tuning the biodistribution of such systems organism at level remains a major obstacle. Here, we constructed targetable DNA origami, analyzed transparent mice, addition to studying tolerability, clearance kinetics, immune response parameters. Untargeted origami primarily accumulated spleen liver, while an cell-targeting...
Abstract Freshmen officer cadets in a military university participating 6 weeks of induction undergo strenuous physical training. Vigorous training has been shown to affect red and white blood cell platelet parameters. Effects have related the mode exercise involved. Since encompassed various modes exercise, we studied effect on basic anthropometric hematologic Twenty-seven healthy freshmen (13 male 14 female) joined study. A sample measures were collected before into program. An automated...