Laura M. Bartos
A5081627019- Glioma Diagnosis and Treatment
- Neuroinflammation and Neurodegeneration Mechanisms
- Anesthesia and Neurotoxicity Research
- Immune cells in cancer
- Medical Imaging Techniques and Applications
- Radiomics and Machine Learning in Medical Imaging
- Radiopharmaceutical Chemistry and Applications
- Alzheimer's disease research and treatments
- Drug Transport and Resistance Mechanisms
- Single-cell and spatial transcriptomics
- Neurological Disease Mechanisms and Treatments
- Inflammation biomarkers and pathways
- CAR-T cell therapy research
- Multiple Sclerosis Research Studies
- Lysosomal Storage Disorders Research
- Advanced Neuroimaging Techniques and Applications
- Sarcoma Diagnosis and Treatment
- S100 Proteins and Annexins
- Chemokine receptors and signaling
- RNA Research and Splicing
- Extracellular vesicles in disease
- Brain Metastases and Treatment
- Autoimmune and Inflammatory Disorders Research
- Neuroendocrine Tumor Research Advances
- Peroxisome Proliferator-Activated Receptors
Ludwig-Maximilians-Universität München
2021-2025
Klinik und Poliklinik für Nuklearmedizin
2022-2025
LMU Klinikum
2022-2024
München Klinik
2022-2023
IRCCS Ospedale San Raffaele
2013
Vita-Salute San Raffaele University
2013
Abstract Loss-of-function variants of TREM2 are associated with increased risk Alzheimer’s disease (AD), suggesting that activation this innate immune receptor may be a useful therapeutic strategy. Here we describe high-affinity human TREM2-activating antibody engineered monovalent transferrin (TfR) binding site, termed transport vehicle (ATV), to facilitate blood–brain barrier transcytosis. Upon peripheral delivery in mice, ATV:TREM2 showed improved brain biodistribution and enhanced...
The bone marrow in the skull is important for shaping immune responses brain and meninges, but its molecular makeup among bones relevance human diseases remain unclear. Here, we show that mouse has most distinct transcriptomic profile compared with other states of health injury, characterized by a late-stage neutrophil phenotype. In humans, proteome analysis reveals distinct, differentially expressed neutrophil-related pathways unique synaptic protein signature. 3D imaging demonstrates...
In Assessment of OraL Laquinimod in PrEventing ProGRession Multiple SclerOsis (ALLEGRO), a phase III study relapsing-remitting multiple sclerosis (RRMS), oral laquinimod slowed disability and brain atrophy progression, suggesting may reduce tissue damage MS. MRI techniques sensitive to the most destructive aspects disease were used further investigate laquinimod's potential effects on inflammation neurodegeneration.1106 RRMS patients randomised 1:1 receive once-daily (0.6 mg) or placebo for...
We aimed to investigate the impact of microglial activity and FDG uptake on metabolic connectivity, since activation states determine FDG-PET alterations. Metabolic connectivity refers a concept interacting brain regions receives growing interest in approaching complex cerebral networks neurodegenerative diseases. However, underlying sources remain be elucidated.We analyzed measured by interregional correlation coefficients (ICCs) scans WT mice with mutations progranulin (Grn) or triggering...
According to the World Health Organization classification for tumors of central nervous system, mutation status isocitrate dehydrogenase (IDH) genes has become a major diagnostic discriminator gliomas. Therefore, imaging-based prediction IDH is high interest individual patient management. We compared and evaluated value radiomics derived from dual positron emission tomography (PET) magnetic resonance imaging (MRI) data predict non-invasively.
The 18-kDa translocator protein (TSPO) is gaining recognition as a relevant target in glioblastoma imaging. However, data on the potential prognostic value of TSPO PET imaging are lacking. Therefore, we investigated association results with survival outcome homogeneous cohort patients. Methods: Patients were included who had newly diagnosed, histologically confirmed isocitrate dehydrogenase (IDH)-wild-type available before either normofractionated radiotherapy combined temozolomide or...
Various cellular sources hamper interpretation of positron emission tomography (PET) biomarkers in the tumor microenvironment (TME). We developed an approach immunomagnetic cell sorting after vivo radiotracer injection (scRadiotracing) with three-dimensional (3D) histology to dissect allocation PET signals TME. In mice implanted glioblastoma, translocator protein (TSPO) uptake per was higher compared tumor-associated microglia/macrophages (TAMs), validated by levels. Translation vitro...
Abstract Triggering receptor expressed on myeloid cells 2 (TREM2) is a central regulator of microglial activity and sequence variants are major risk factors for late onset Alzheimer’s disease (LOAD). To better understand the molecular functional changes associated with TREM2 signalling, we generated reporter mouse model observed gradual upregulation expression increasing plaque proximity. Isolated microglia were sorted based their transcriptomic profiles acquired in both wildtype APP...
Ziel/Aim: [18F]D2-Deprenyl bindet an das Enzym MAO-B und ermöglicht so Rückschlüsse auf die Aktivität von Astrozyten, als Index der Neuroinflammation im ZNS. Bislang wurde [18F]D2-Deprenyl-PET vor allem zur Untersuchung neurodegenerativen Erkrankungen wie Alzheimer ALS eingesetzt. Glioblastome sind häufigsten primären Hirntumore. Da sie entarteten Astrozyten ausgehen könnte eine Bildgebung durch auch hier Nutzen sein, jedoch Erfassung dienen.
With great interest, our independent groups of scientists located in Korea and Germany recognized the use a very similar methodologic approach to quantify uptake radioactive glucose (<sup>18</sup>F-FDG) at cellular level. The focus investigations was disentangle microglial <sup>18</sup>F-FDG uptake. To do so, CD11b immunomagnetic cell sorting applied isolate microglia cells after in vivo injection, allow simple quantification via γ-counter. Importantly, this technique reveals snapshot...
Glioma patients, especially recurrent glioma, suffer from a poor prognosis. While advances to classify glioma on molecular level improved prognostication at initial diagnosis, markers prognosticate survival in the situation are still needed. As 18 kDa translocator protein (TSPO) was previously reported be associated with aggressive histopathological features, we correlated TSPO positron emission tomography (PET) signal using [18F]GE180 large cohort of patients their clinical outcome.In PET...
Abstract TSPO is a promising novel tracer target for positron-emission tomography (PET) imaging of brain tumors. However, due to the heterogeneity cell populations that contribute TSPO-PET signal, interpretation may be challenging. We therefore evaluated enrichment/expression in connection with its underlying histopathological and molecular features gliomas. analyzed expression regulatory mechanisms large silico datasets by performing direct bisulfite sequencing promotor. In glioblastoma...
Summary Tau-PET receives growing interest as an imaging biomarker for the 4-repeat tauopathy progressive supranuclear palsy (PSP). However, translation of in vitro 4R-tau binding to vivo tau-PET signals is still unclear. Therefore, we conducted a longitudinal [ 18 F]PI-2620 PET/MRI study 4-repeat-tau mouse model (PS19) and found elevated PET signal presence high neuronal tau. Cell sorting after radiotracer injection revealed higher tracer uptake single neurons compared astrocytes PS19 mice....
Triggering receptor expressed on myeloid cells 2 (TREM2) plays an essential role in microglia activation and is being investigated as a potential therapeutic target for modulation of several neurological diseases. In this study, we present the development preclinical evaluation
Abstract Tau PET has attracted increasing interest as an imaging biomarker for 4-repeat (4R)-tauopathy progressive supranuclear palsy (PSP). However, the translation of in vitro 4R-tau binding to vivo tau signals is still unclear. Therefore, we performed a translational study using broad spectrum advanced methodologies investigate sources [ 18 F]PI-2620 individuals with 4R-tauopathies, including pilot autopsy patients. First, conducted longitudinal PET/MRI 4-repeat-tau mouse model (PS19) and...
The 18 kDa translocator protein (TSPO) is increasingly recognized as an interesting target for the imaging of glioblastoma (GBM). Here, we investigated TSPO PET and autoradiography in frequently used GL261 mouse model aimed to generate insights into temporal evolution radioligand uptake a preclinical setting. We performed longitudinal [18F]GE-180 study from day 4 14 post inoculation orthotopic syngeneic GBM (n = 21 mice, n 3 sham mice). Contrast-enhanced computed tomography (CT) was at final...
SUMMARY The meninges of the brain are an important component neuroinflammatory response. Diverse immune cells move from calvaria marrow into dura mater via recently discovered skull-meninges connections (SMCs). However, how bone is different other bones and whether it contributes to human diseases remain unknown. Using multi-omics approaches whole mouse transparency we reveal that highly heterogeneous across body. harbors most distinct molecular signature with hundreds differentially...
Introduction The 18 kDa translocator protein (TSPO) receives growing interest as a biomarker in glioblastoma. Mouse models can serve an important tool for the investigation of biomarkers glioblastoma, but several glioblastoma indicated only low TSPO-PET signals contrast to high human Thus, we aimed investigate imaging syngeneic immunocompetent SB28 mouse model, which is thought closely represent tumor microenvironment (TME) Methods Dynamic TSPO-PET/CT was performed 60 min after injection...
Niemann-Pick type C (NPC) disease is an inherited lysosomal storage disorder mainly driven by mutations in the NPC1 gene, causing lipid accumulation within late endosomes/lysosomes and resulting progressive neurodegeneration. Although microglial activation precedes neuronal loss, it remains elusive whether loss of membrane protein microglia actively contributes to NPC pathology. In a mouse model with depletion myeloid cells, we report severe alterations lipidomic profiles, including...
Abstract Various cellular sources hamper interpretation of positron-emission-tomography (PET) biomarkers in the tumor microenvironment (TME). We developed immunomagnetic cell sorting after vivo radiotracer injection (scRadiotracing) combination with 3D-histology via tissue clearing to dissect allocation PET signals TME. In SB28 glioblastoma mice, translocator protein (TSPO) uptake per was higher compared tumor-associated microglia/macrophages (TAMs). Cellular validated by proteomics and...