A5032644974- Lipid Membrane Structure and Behavior
- CRISPR and Genetic Engineering
- RNA and protein synthesis mechanisms
- Nanopore and Nanochannel Transport Studies
- Advanced biosensing and bioanalysis techniques
- Advanced Fluorescence Microscopy Techniques
- Epigenetics and DNA Methylation
- Cellular transport and secretion
- Cancer Cells and Metastasis
- Genomics and Phylogenetic Studies
- Cancer Genomics and Diagnostics
- RNA Interference and Gene Delivery
- Genomics and Chromatin Dynamics
- Single-cell and spatial transcriptomics
- Bacteriophages and microbial interactions
- Cancer Research and Treatments
- Microbial Metabolic Engineering and Bioproduction
- Animal Genetics and Reproduction
- Cell Image Analysis Techniques
- Viral Infectious Diseases and Gene Expression in Insects
- Protein Structure and Dynamics
- Protist diversity and phylogeny
- RNA modifications and cancer
- Ubiquitin and proteasome pathways
- Chromosomal and Genetic Variations
Helmholtz Zentrum München
2023-2025
Harvard University Press
2015
University of Cambridge
2015
The Nature Conservancy
2013
Pediatrics and Genetics
2008
University of Gothenburg
2004-2005
Max Planck Institute of Biophysics
2004
European Bioinformatics Institute
2003
University of Göttingen
1993
The bone marrow in the skull is important for shaping immune responses brain and meninges, but its molecular makeup among bones relevance human diseases remain unclear. Here, we show that mouse has most distinct transcriptomic profile compared with other states of health injury, characterized by a late-stage neutrophil phenotype. In humans, proteome analysis reveals distinct, differentially expressed neutrophil-related pathways unique synaptic protein signature. 3D imaging demonstrates...
Abstract Whole-body imaging techniques play a vital role in exploring the interplay of physiological systems maintaining health and driving disease. We introduce wildDISCO, new approach for whole-body immunolabeling, optical clearing mice, circumventing need transgenic reporter animals or nanobody labeling so overcoming existing technical limitations. identified heptakis(2,6-di- O -methyl)-β-cyclodextrin as potent enhancer cholesterol extraction membrane permeabilization, enabling deep,...
SARS-CoV-2 infection is associated with long-lasting neurological symptoms, although the underlying mechanisms remain unclear. Using optical clearing and imaging, we observed accumulation of spike protein in skull-meninges-brain axis human COVID-19 patients, persisting long after viral clearance. Further, biomarkers neurodegeneration were elevated cerebrospinal fluid from COVID proteomic analysis skull, meninges, brain samples revealed dysregulated inflammatory pathways...
Efficient and accurate nanocarrier development for targeted drug delivery is hindered by a lack of methods to analyze its cell-level biodistribution across whole organisms. Here we present Single Cell Precision Nanocarrier Identification (SCP-Nano), an integrated experimental deep learning pipeline comprehensively quantify the targeting nanocarriers throughout mouse body at single-cell resolution. SCP-Nano reveals tissue distribution patterns lipid nanoparticles (LNPs) after different...
We have investigated the role for diacylglycerol (DAG) in membrane bud formation Golgi apparatus. Addition of propranolol to specifically inhibit phosphatidate phosphohydrolase (PAP), an enzyme responsible converting phosphatidic acid into DAG, effectively prevents buds. The effect PAP inhibition on membranes is rapid and occurs within 3 min. Removal inhibitor then results a burst buds, vesicles, tubules that peaks 2 inability form buds presence does not appear be correlated with loss...
Many diseases, such as obesity, have systemic effects that impact multiple organ systems throughout the body. However, tools for comprehensive, high-resolution analysis of disease-associated changes at whole-body scale been lacking. Here, we developed a suite deep learning-based image algorithms (MouseMapper) and integrated it with tissue clearing light-sheet microscopy to enable comprehensive diseases impacting diverse across mouse This approach enables quantitative cellular structural...
Abstract Specific non‐covalent interactions between transmembrane (TM) α‐helices are important in a variety of biological processes. Experimental and computational studies have shown that van der Waals play an role the tight packing TM α‐helices, although polar can also be some instances. Based on assumption interaction alone is sufficient for meso‐scale (residue‐scale) description we designed novel residue‐scale scoring function modeling structures oligomers α‐helices. We first calculated...