Artem Zatcepin
A5025850978- Neuroinflammation and Neurodegeneration Mechanisms
- Glioma Diagnosis and Treatment
- Alzheimer's disease research and treatments
- Anesthesia and Neurotoxicity Research
- Medical Imaging Techniques and Applications
- Immune cells in cancer
- Radiomics and Machine Learning in Medical Imaging
- S100 Proteins and Annexins
- Neurological Disease Mechanisms and Treatments
- Atomic and Subatomic Physics Research
- Radiation Detection and Scintillator Technologies
- Immune Response and Inflammation
- Single-cell and spatial transcriptomics
- Sarcoma Diagnosis and Treatment
- Advanced MRI Techniques and Applications
- Extracellular vesicles in disease
- interferon and immune responses
- Functional Brain Connectivity Studies
- Advanced X-ray and CT Imaging
- Inflammation biomarkers and pathways
- Advanced Neuroimaging Techniques and Applications
- Additive Manufacturing and 3D Printing Technologies
- Medical Imaging and Analysis
- Stroke Rehabilitation and Recovery
- Amyotrophic Lateral Sclerosis Research
Ludwig-Maximilians-Universität München
2020-2025
Klinik und Poliklinik für Nuklearmedizin
2023-2025
German Center for Neurodegenerative Diseases
2021-2024
LMU Klinikum
2020-2024
München Klinik
2023
Klinikum rechts der Isar
2020
Technical University of Munich
2020
2-Deoxy-2-[18F]fluoro-d-glucose positron emission tomography (FDG-PET) is widely used to study cerebral glucose metabolism. Here, we investigated whether the FDG-PET signal directly influenced by microglial uptake in mouse models and patients with neurodegenerative diseases. Using a recently developed approach for cell sorting after FDG injection, found that, at cellular resolution, microglia displayed higher than neurons astrocytes. Alterations were responsible both decrease Trem2-deficient...
The bone marrow in the skull is important for shaping immune responses brain and meninges, but its molecular makeup among bones relevance human diseases remain unclear. Here, we show that mouse has most distinct transcriptomic profile compared with other states of health injury, characterized by a late-stage neutrophil phenotype. In humans, proteome analysis reveals distinct, differentially expressed neutrophil-related pathways unique synaptic protein signature. 3D imaging demonstrates...
Genetic mutations underlying familial Alzheimer's disease (AD) were identified decades ago, but the field is still in search of transformative therapies for patients. While mouse models based on overexpression mutated transgenes have yielded key insights mechanisms disease, those are subject to artifacts, including random genetic integration transgene, ectopic expression and non-physiological protein levels. The engineering novel using knock-in approaches addresses some limitations. With...
Haploinsufficiency of the progranulin (PGRN)‐encoding gene (GRN) causes frontotemporal lobar degeneration (GRN‐FTLD) and results in microglial hyperactivation, TREM2 activation, lysosomal dysfunction, TDP‐43 deposition. To understand contribution hyperactivation to pathology, we used genetic pharmacological approaches suppress TREM2‐dependent transition microglia from a homeostatic disease‐associated state. Trem2 deficiency Grn KO mice reduced hyperactivation. explore antibody‐mediated...
Reactive gliosis is a common pathological hallmark of CNS pathology resulting from neurodegeneration and neuroinflammation. In this study we investigate the capability novel monoamine oxidase B (MAO-B) PET ligand to monitor reactive astrogliosis in transgenic mouse model Alzheimer`s disease (AD). Furthermore, performed pilot patients with range neurodegenerative neuroinflammatory conditions.A cross-sectional cohort 24 (PS2APP) 25 wild-type mice (age range: 4.3-21.0 months) underwent 60 min...
We aimed to investigate the impact of microglial activity and FDG uptake on metabolic connectivity, since activation states determine FDG-PET alterations. Metabolic connectivity refers a concept interacting brain regions receives growing interest in approaching complex cerebral networks neurodegenerative diseases. However, underlying sources remain be elucidated.We analyzed measured by interregional correlation coefficients (ICCs) scans WT mice with mutations progranulin (Grn) or triggering...
According to the World Health Organization classification for tumors of central nervous system, mutation status isocitrate dehydrogenase (IDH) genes has become a major diagnostic discriminator gliomas. Therefore, imaging-based prediction IDH is high interest individual patient management. We compared and evaluated value radiomics derived from dual positron emission tomography (PET) magnetic resonance imaging (MRI) data predict non-invasively.
Abstract Patients with Alzheimer’s disease (AD) and clinically overlapping neurodegenerative diseases are classified molecularly using the A/T/N classification system. Apart from fluid biomarkers structural MRI, three-dimensional system incorporates characteristic features β-amyloid-PET (A), tau-PET (T), FDG-PET (N). We evaluated if dynamic of [ 18 F]PI-2620 allow assessment in individual patients a single imaging session. Cortical tissue clearance (K2a) was validated as surrogate β-amyloid...
Various cellular sources hamper interpretation of positron emission tomography (PET) biomarkers in the tumor microenvironment (TME). We developed an approach immunomagnetic cell sorting after vivo radiotracer injection (scRadiotracing) with three-dimensional (3D) histology to dissect allocation PET signals TME. In mice implanted glioblastoma, translocator protein (TSPO) uptake per was higher compared tumor-associated microglia/macrophages (TAMs), validated by levels. Translation vitro...
Abstract Purpose [ 18 F]PI-2620 positron emission tomography (PET) detects misfolded tau in progressive supranuclear palsy (PSP) and Alzheimer’s disease (AD). We questioned the feasibility value of absolute PET quantification for assessing by regional distribution volumes (V T ). Here, arterial input functions (AIF) represent gold standard, but cannot be applied routine clinical practice, whereas image-derived (IDIF) a non-invasive alternative. aimed to validate IDIF against AIF we evaluated...
Among functional imaging methods, metabolic connectivity (MC) is increasingly used for investigation of regional network changes to examine the pathophysiology neurodegenerative diseases such as Alzheimer's disease (AD) or movement disorders. Hitherto, MC was mostly in clinical studies, but only a few studies demonstrated usefulness rodent brain. The goal current work analyze and validate alterations three different mouse models (β-amyloid tau) by use 2-deoxy-2-[18F]fluoro-d-glucose positron...
β-amyloid (Aβ) small animal PET facilitates quantification of fibrillar amyloidosis in Alzheimer's disease (AD) mouse models. Thus, the methodology is receiving growing interest as a monitoring tool preclinical drug trials. In this regard, harmonization data from different scanners at multiple sites would allow establishment large collaborative cohorts and may facilitate efficacy comparison treatments. Therefore, we objected to determine level agreement Aβ-PET by head-to-head three...
Parallax error is a common issue in high-resolution preclinical positron emission tomography (PET) scanners as well clinical that have long axial field of view (FOV), which increases estimation uncertainty the annihilation position and therefore degrades spatial resolution. A way to address this depth-of-interaction (DOI) estimation. In work we propose two machine learning-based algorithms, dense convolutional neural network (NN), multiple linear regression (MLR)-based method estimate DOI...
SUMMARY The meninges of the brain are an important component neuroinflammatory response. Diverse immune cells move from calvaria marrow into dura mater via recently discovered skull-meninges connections (SMCs). However, how bone is different other bones and whether it contributes to human diseases remain unknown. Using multi-omics approaches whole mouse transparency we reveal that highly heterogeneous across body. harbors most distinct molecular signature with hundreds differentially...
Neuroinflammation evaluation after acute ischemic stroke is a promising option for selecting an appropriate post-stroke treatment strategy. To assess neuroinflammation in vivo, translocator protein PET (TSPO PET) can be used. However, the gold standard TSPO quantification method includes 90 min scan and continuous arterial blood sampling, which challenging to perform on routine basis. In this work, we determine what information required simplified approach using machine learning algorithm....
Introduction The 18 kDa translocator protein (TSPO) receives growing interest as a biomarker in glioblastoma. Mouse models can serve an important tool for the investigation of biomarkers glioblastoma, but several glioblastoma indicated only low TSPO-PET signals contrast to high human Thus, we aimed investigate imaging syngeneic immunocompetent SB28 mouse model, which is thought closely represent tumor microenvironment (TME) Methods Dynamic TSPO-PET/CT was performed 60 min after injection...
Understanding neuroinflammation after acute ischemic stroke is a crucial step on the way to an individualized post-stroke treatment. Microglia activation, essential part of neuroinflammation, can be assessed using [18F]GE-180 18 kDa translocator protein positron emission tomography (TSPO-PET). However, commonly used 60-90 min post-injection (p.i.) time window was not yet proven suitable for assessment. In this study, we compare semi-quantitative estimates derived from late frames...
Abstract Various cellular sources hamper interpretation of positron-emission-tomography (PET) biomarkers in the tumor microenvironment (TME). We developed immunomagnetic cell sorting after vivo radiotracer injection (scRadiotracing) combination with 3D-histology via tissue clearing to dissect allocation PET signals TME. In SB28 glioblastoma mice, translocator protein (TSPO) uptake per was higher compared tumor-associated microglia/macrophages (TAMs). Cellular validated by proteomics and...
Current therapy strategies still provide only limited success in the treatment of glioblastoma, most frequent primary brain tumor adults. In addition to characterization microenvironment, global changes patients with glioblastoma have been described. However, impact and molecular signature neuroinflammation distant site not yet thoroughly elucidated.
<p>Contralateral hemispheres of SB28 glioblastoma mice indicate signatures disease-associated myeloid cells and immunosuppression. <b>A,</b> Volcano plot highlights genes with significant upregulation downregulation in the contralateral hemisphere (red blue, respectively) compared to sham. <b>B,</b> qPCR reveals higher expression related an inflammatory cell state contrast <b>C,</b> Illustration 50 pathways highest elevation immune (informed by IPA)....
<p>Characteristics of the cohort.</p>
<p>Elevated TSPO-PET signals in the non-lesional hemisphere of patients with glioblastoma. <b>A,</b> Patient selection process. Patients receiving imaging at initial diagnosis glioma were allocated. unilateral manifestation glioblastoma (WHO IV; <i>n</i> = 41) or isocitrate dehydrogenase mutant astrocytoma WHO grade 2 (IDHmut 2; 7) time PET selected for further analysis. <b>B,</b> newly diagnosed but not IDHmut indicate higher signal contralateral...