A5024120001- Cancer-related molecular mechanisms research
- RNA modifications and cancer
- Atherosclerosis and Cardiovascular Diseases
- MicroRNA in disease regulation
- Circular RNAs in diseases
- Tryptophan and brain disorders
- Stress Responses and Cortisol
- RNA Research and Splicing
- Metabolomics and Mass Spectrometry Studies
- Aortic aneurysm repair treatments
- Cell Adhesion Molecules Research
- Genetic Associations and Epidemiology
- Antiplatelet Therapy and Cardiovascular Diseases
- Immune cells in cancer
- Immune Response and Inflammation
- Neutrophil, Myeloperoxidase and Oxidative Mechanisms
- Advanced Proteomics Techniques and Applications
- Aortic Disease and Treatment Approaches
- Cholesterol and Lipid Metabolism
- Eicosanoids and Hypertension Pharmacology
- Galectins and Cancer Biology
- Calcium signaling and nucleotide metabolism
- Congenital heart defects research
- SARS-CoV-2 and COVID-19 Research
- Mass Spectrometry Techniques and Applications
Ludwig-Maximilians-Universität München
2016-2025
LMU Klinikum
2016-2025
München Klinik
2014-2022
Leipzig University
2010-2017
University Hospital Leipzig
2007-2016
German Centre for Cardiovascular Research
2016
Kiel University
2016
ETH Zurich
2016
University of Ottawa
2016
New York University
2016
Abstract Circular RNAs (circRNAs) are broadly expressed in eukaryotic cells, but their molecular mechanism human disease remains obscure. Here we show that circular antisense non-coding RNA the INK4 locus ( circANRIL ), which is transcribed at a of atherosclerotic cardiovascular on chromosome 9p21, confers atheroprotection by controlling ribosomal (rRNA) maturation and modulating pathways atherogenesis. CircANRIL binds to pescadillo homologue 1 (PES1), an essential 60S-preribosomal assembly...
Proteins in the circulatory system mirror an individual's physiology. In daily clinical practice, protein levels are generally determined using single-protein immunoassays. High-throughput, quantitative analysis mass-spectrometry-based proteomics of blood, plasma, and serum would be advantageous but is challenging because high dynamic range abundances. Here, we introduce a rapid robust "plasma proteome profiling" pipeline. This single-run shotgun proteomic workflow does not require depletion...
We tested the hypothesis that expression of transcripts adjacent to chromosome 9p21 (Chr9p21) locus coronary artery disease was affected by genotype at this and associated with atherosclerosis risk.We replicated for (P=0.007; OR=1.28) other manifestations such as carotid plaque (P=0.003; OR=1.31) in Leipzig Heart Study, a cohort 1134 patients varying degree angiographically assessed disease. Expression analysis peripheral blood mononuclear cells (n=1098) revealed EU741058 NR_003529 antisense...
Context Chronic systemic inflammation in obesity originates from local immune responses visceral adipose tissue. However, assessment of a broad range inflammation-mediating cytokines and their relationship to physical activity adipometrics has scarcely been reported date. Objective To characterize the profile pro- anti-inflammatory impact energy expenditure individuals with general obesity, central non-obese subjects. Design, Setting, Participants A cross-sectional study comprising 117 obese...
The chromosome 9p21 (Chr9p21) locus of coronary artery disease has been identified in the first surge genome-wide association and is strongest genetic factor atherosclerosis known today. Chr9p21 encodes long non-coding RNA (ncRNA) antisense INK4 (ANRIL). ANRIL expression associated with genotype correlated severity. Here, we report on molecular mechanisms through which regulates target-genes trans, leading to increased cell proliferation, adhesion decreased apoptosis, are all essential...
Abstract Numerous high-throughput sequencing studies have focused on detecting conventionally spliced mRNAs in RNA-seq data. However, non-standard RNAs arising through gene fusion, circularization or trans-splicing are often neglected. We introduce a novel, unbiased algorithm to detect splice junctions from single-end cDNA sequences. In contrast other methods, our approach accommodates multi-junction structures. Our method compares favorably with competing tools for and, gain of up 40%...
Recovery after stroke is a multicellular process encompassing neurons, resident immune cells, and brain-invading cells. Stroke alters the gut microbiome, which in turn has considerable impact on outcome. However, mechanisms underlying gut–brain interaction implications for long-term recovery are largely elusive. Here, we tested hypothesis that short-chain fatty acids (SCFAs), key bioactive microbial metabolites, missing link along axis might be able to modulate experimental stroke. SCFA...
Report30 September 2019Open Access Transparent process Plasma Proteome Profiling to detect and avoid sample-related biases in biomarker studies Philipp E Geyer orcid.org/0000-0001-7980-4826 Department of Proteomics Signal Transduction, Max Planck Institute Biochemistry, Martinsried, Germany NNF Center for Protein Research, Faculty Health Sciences, University Copenhagen, Denmark Search more papers by this author Eugenia Voytik Peter V Treit Sophia Doll Alisa Kleinhempel Laboratory Medicine,...
Currently, there are no blood-based biomarkers with clinical utility for acute ischemic stroke (IS). MicroRNAs show promise as disease markers because of their cell type-specific expression patterns and stability in peripheral blood.To identify circulating microRNAs associated IS, determine temporal course up to 90 days post-stroke, explore an early diagnostic marker.We used RNA sequencing study changes a discovery sample 20 patients IS matched healthy control subjects. We further applied...
A deeper understanding of COVID-19 on human molecular pathophysiology is urgently needed as a foundation for the discovery new biomarkers and therapeutic targets. Here we applied mass spectrometry (MS)-based proteomics to measure serum proteomes patients symptomatic, but PCR-negative controls, in time-resolved manner. In 262 controls 458 longitudinal samples 31 patients, hospitalized COVID-19, remarkable 26% proteins changed significantly. Bioinformatics analyses revealed co-regulated groups...
Epigenetic mechanisms might be involved in the regulation of interindividual lipid level variability and thus may contribute to cardiovascular risk profile. The aim this study was investigate association between genome-wide DNA methylation blood levels high-density lipoprotein cholesterol, low-density triglycerides, total cholesterol. Observed changes were also further analyzed examine their relationship with previous hospitalized myocardial infarction.Genome-wide patterns determined whole...
Background— Phytosterols are plant-derived sterols that taken up from food and can serve as biomarkers of cholesterol uptake. Serum levels under tight genetic control. We used a genomic approach to study the molecular regulation serum phytosterol potential links coronary artery disease (CAD). Methods Results— A genome-wide association for phytosterols (campesterol, sitosterol, brassicasterol) was conducted in population-based sample KORA (Cooperative Research Region Augsburg) (n=1495) with...
Background: The CXCL12/CXCR4 chemokine ligand/receptor axis controls (progenitor) cell homeostasis and trafficking. So far, an atheroprotective role of has only been implied through pharmacological intervention, in particular, because the somatic deletion CXCR4 gene mice is embryonically lethal. Moreover, cell-specific effects arterial wall underlying mechanisms remain elusive, prompting us to investigate relevance vascular types for atheroprotection. Methods: We examined atherosclerosis...
// Erik Biros 1,* , Gabor Gäbel 2,* Corey S. Moran 1 Charlotte Schreurs 3 Jan H. N. Lindeman Philip J. Walker 4 Maria Nataatmadja 5 Malcolm West Lesca M. Holdt 6 Irene Hinterseher 7 Christian Pilarsky 8 and Jonathan Golledge 1,9 The Queensland Research Centre for Peripheral Vascular Disease, College of Medicine Dentistry, James Cook University, Townsville, Queensland, Australia 2 Department Endovascular Surgery, Ludwig-Maximillian Munich, Germany Leiden University Medical Center,...