A5075486416- Sirtuins and Resveratrol in Medicine
- Calcium signaling and nucleotide metabolism
- Psychotherapy Techniques and Applications
- PARP inhibition in cancer therapy
- Genetics and Neurodevelopmental Disorders
- Adipose Tissue and Metabolism
- Biochemical and Molecular Research
- Forensic and Genetic Research
- Autophagy in Disease and Therapy
- Peptidase Inhibition and Analysis
- Epigenetics and DNA Methylation
- Ubiquitin and proteasome pathways
- Molecular Biology Techniques and Applications
- RNA modifications and cancer
- Mitochondrial Function and Pathology
- Fungal and yeast genetics research
- Academic and Historical Perspectives in Psychology
- Adenosine and Purinergic Signaling
- Genetics, Aging, and Longevity in Model Organisms
- Tryptophan and brain disorders
- Genetics, Bioinformatics, and Biomedical Research
- Psychoanalysis and Psychopathology Research
- Epilepsy research and treatment
- Cancer-related gene regulation
- Cytomegalovirus and herpesvirus research
University of Iowa
2014-2024
Beckman Research Institute
2022-2024
City of Hope
2022-2024
City Of Hope National Medical Center
2020-2024
Parsons (United States)
2023
The Wallace H. Coulter Department of Biomedical Engineering
2023
Georgia Institute of Technology
2022-2023
University of California, Berkeley
2001-2021
Carver Bible College
2010-2014
The University of Texas Health Science Center at San Antonio
1998-2013
Morpholino phosphorodiamidate antisense oligonucleotides (MOs) and short interfering RNAs (siRNAs) are commonly used platforms to study gene function by sequence-specific knockdown. Both technologies, however, can elicit undesirable off-target effects. We have several model genes these effects in detail the zebrafish, Danio rerio. Using zebrafish embryo as a template, correct mistargeting readily discernible through direct comparison of MO-injected animals with well-studied mutants. show...
Abstract Nicotinamide riboside (NR) is in wide use as an NAD + precursor vitamin. Here we determine the time and dose-dependent effects of NR on blood metabolism humans. We report that human can rise much 2.7-fold with a single oral dose pilot study one individual, elevates mouse hepatic distinct superior pharmacokinetics to those nicotinic acid nicotinamide. further show doses 100, 300 1,000 mg produce increases metabolome first clinical trial also adenine dinucleotide (NAAD), which was not...
Nicotinamide adenine dinucleotide (NAD+) is modulated by conditions of metabolic stress and has been reported to decline with aging in preclinical models, but human data are sparse. riboside (NR) supplementation ameliorates dysfunction rodents. We aimed establish whether oral NR aged participants can increase the skeletal muscle NAD+ metabolome if it alter mitochondrial bioenergetics. supplemented 12 men 1 g per day for 21 days a placebo-controlled, randomized, double-blind, crossover trial....
Abstract NAD + is a vital redox cofactor and substrate required for activity of various enzyme families, including sirtuins poly(ADP-ribose) polymerases. Supplementation with precursors, such as nicotinamide mononucleotide (NMN) or riboside (NR), protects against metabolic disease, neurodegenerative disorders age-related physiological decline in mammals. Here we show that kinase 1 (NRK1) necessary rate-limiting the use exogenous NR NMN synthesis. Using genetic gain- loss-of-function models,...
Background: Myocardial metabolic impairment is a major feature in chronic heart failure. As the coenzyme fuel oxidation and oxidative phosphorylation substrate for enzymes signaling energy stress response, nicotinamide adenine dinucleotide (NAD + ) emerging as target number of diseases including Little known on mechanisms regulating homeostasis NAD failing heart. Methods: To explore possible alterations heart, we quantified expression biosynthetic human mouse model dilated cardiomyopathy...
Abstract Male C57BL/6J mice raised on high fat diet (HFD) become prediabetic and develop insulin resistance sensory neuropathy. The same given low doses of streptozotocin are a model type 2 diabetes (T2D), developing hyperglycemia, severe diabetic peripheral neuropathy involving motor neurons. Because suggestions that increased NAD + metabolism might address glycemic control be neuroprotective, we treated T2D with nicotinamide riboside (NR) added to HFD. NR improved glucose tolerance,...
Poly(ADP-ribose) polymerase (PARP) superfamily members covalently link either a single ADP-ribose (ADPR) or chain of ADPR units to proteins using NAD as the source ADPR. Although well-known poly(ADP-ribosylating) (PARylating) PARPs primarily function in DNA damage response, many noncanonical mono(ADP-ribosylating) (MARylating) are associated with cellular antiviral responses. We recently demonstrated robust up-regulation several following infection murine hepatitis virus (MHV), model...
Nicotinamide riboside (NR) is a newly discovered nicotinamide adenine dinucleotide (NAD+) precursor vitamin. A crystal form of NR chloride termed NIAGEN generally recognized as safe (GRAS) for use in foods and the subject two New Dietary Ingredient Notifications dietary supplements. To evaluate kinetics dose-dependency oral availability safety overweight, but otherwise healthy men women, an 8-week randomized, double-blind, placebo-controlled clinical trial was conducted. Consumption 100, 300...
Nicotinamide adenine dinucleotide (NAD+) precursor nicotinamide riboside (NR) has emerged as a promising compound to improve obesity-associated mitochondrial dysfunction and metabolic syndrome in mice. However, most short-term clinical trials conducted so far have not reported positive outcomes. Therefore, we aimed determine whether long-term NR supplementation boosts biogenesis health humans. Twenty body mass index (BMI)-discordant monozygotic twin pairs were supplemented with an escalating...