A5090970318- Gut microbiota and health
- Diet and metabolism studies
- Adipose Tissue and Metabolism
- Liver Disease Diagnosis and Treatment
- Metabolomics and Mass Spectrometry Studies
- Muscle metabolism and nutrition
- Exercise and Physiological Responses
- Adipokines, Inflammation, and Metabolic Diseases
- Muscle Physiology and Disorders
- Dietary Effects on Health
- Metabolism and Genetic Disorders
- Biochemical and Molecular Research
- Diabetes and associated disorders
- Cancer-related cognitive impairment studies
- Cannabis and Cannabinoid Research
- Sleep and Wakefulness Research
- Neurogenesis and neuroplasticity mechanisms
- Lipid metabolism and disorders
- Enzyme Structure and Function
- Cancer, Hypoxia, and Metabolism
- Erythrocyte Function and Pathophysiology
- Stress Responses and Cortisol
- Autophagy in Disease and Therapy
- Sirtuins and Resveratrol in Medicine
- Diet, Metabolism, and Disease
University of Jyväskylä
2016-2025
Turku University Hospital
2020-2025
University of Turku
2015-2023
Department of Medical Sciences
2013-2023
Centro de Investigacion Principe Felipe
2009-2013
Zero to Three
2010
Key points Contradictory findings have been reported concerning the function of irisin and its precursor gene, skeletal muscle FNDC5 , in energy homeostasis metabolic health, associated regulatory role exercise PGC‐1α . We analysed effects different short‐ long‐term regimens on serum irisin, studied associations with health parameters. did not change after acute aerobic, endurance training or combined resistance (RE) training, associate disturbances. A single RE bout increased mRNA young,...
Recent studies suggest that exercise alters the gut microbiome. We determined whether six-weeks endurance exercise, without changing diet, affected metagenome and systemic metabolites of overweight women. Previously sedentary women (n = 19) underwent a intervention, but two were excluded due to antibiotic therapy. The microbiota composition functions analyzed by 16S rRNA gene amplicon sequencing metagenomics. Body was with DXA X-ray densitometer serum metabolomics NMR metabolomics. Total...
Nicotinamide adenine dinucleotide (NAD+) precursor nicotinamide riboside (NR) has emerged as a promising compound to improve obesity-associated mitochondrial dysfunction and metabolic syndrome in mice. However, most short-term clinical trials conducted so far have not reported positive outcomes. Therefore, we aimed determine whether long-term NR supplementation boosts biogenesis health humans. Twenty body mass index (BMI)-discordant monozygotic twin pairs were supplemented with an escalating...
Next-generation sequencing (NGS) is currently the method of choice for analyzing gut microbiota composition. As composition a potential future target clinical diagnostics, it utmost importance to enhance and optimize NGS analysis procedures. Here, we have analyzed impact DNA extraction selected 16S rDNA primers on results. Bacterial from frozen stool specimens was extracted with 5 commercially available kits. Special attention paid semiautomated methods that could expedite procedure, thus...
Numerous studies have described specific metabolites as biomarkers of severe liver diseases, but very few measured gut microbiota (GM)-produced in fatty disease. We aimed at finding GM signatures and metabolite markers plasma feces related to high fat content. Based on imaging, we divided study participants into low (<5%, LF, n = 25) (>5%, HF, 39) groups. Fecal (LF 14, HF 11, 7) metabolomes subsets were studied using liquid chromatography/high resolution mass spectrometry. The analyzed 16S...
The aim of this study was to investigate whether overweight/obese women in metabolic disorder group (MDG, n = 27) differ their gut microbiota composition from non-metabolic (NMDG, 47) and normal weight (NWG, 11). Gut profiled fecal samples by 16S rRNA fluorescence situ hybridization flow cytometry 85 premenopausal women. Body measured bioimpedance, dietary intakes were collected via food diaries. Standard procedures used assess plasma glucose, serum insulin, lipids, inflammatory status. We...
To identify serum biomarkers through metabolomics approach that distinguishes physically inactive overweight/obese women with metabolic syndrome from those who are metabolically healthy, independent of body weight and fat mass.We applied nuclear magnetic resonance spectroscopy-based profiling fasting samples to examine the differences between 78 previously inactive, mass matched premenopausal without MetS. MetS was defined as presence at least three following five criteria: waist...
The primary aim of the present study was to investigate acute gene expression responses PGC-1 isoforms and PGC-1α target genes related mitochondrial biogenesis (cytochrome C), angiogenesis (VEGF-A), muscle hypertrophy (myostatin), after a resistance or endurance exercise bout. In addition, aimed elucidate whether changes studied transcripts were linked phosphorylation AMPK MAPK p38. Nineteen physically active men divided into (RE, n = 11) (EE, 8) groups. RE group performed leg press (10 × 10...
Insulin resistance is associated adiposity, but the mechanisms are not fully understood. In this study, we aimed to identify early metabolic alterations with insulin in normoglycemic women varying degree of adiposity. One-hundred and ten young middle-aged were divided into low high IR groups based on their median HOMA-IR (0.9 ± 0.4 vs. 2.8 1.2). Body composition was assessed using DXA, skeletal muscle liver fat by proton magnetic resonance spectroscopy, serum metabolites nuclear spectroscopy...
This study aimed at establishing bacterial flagellin-recognizing toll-like receptor 5 (TLR5) as a novel link between gut microbiota composition, adipose tissue inflammation, and obesity. An microarray database was used to compare women having the highest (n = 4, H-TLR) lowest L-TLR) expression levels of TLR5-signaling pathway genes. Gut composition profiled using flow cytometry FISH. Standard laboratory techniques were determine anthropometric clinical variables. In vivo results verified...
The skeleton is recognized as an important player in energy metabolism through its interactions with other tissues. Whether the association of osteocalcin glucose age dependent has not been fully addressed. objective study was to examine age-specific between different forms and adipokines. This a family-based across three generations. conducted at university laboratory. Sixty-four daughter-premenopausal mother-maternal grandmother trios participated study. Fasting plasma insulin...
Understanding the importance of gut microbiota (GM) in non-alcoholic fatty liver disease (NAFLD) has raised hope for therapeutic microbes. We have shown that high hepatic fat content associated with low abundance Faecalibacterium prausnitzii humans and, further, administration F. prevented NAFLD mice. Here, we aimed at targeting by prebiotic xylo-oligosaccharides (XOS) to treat NAFLD. First, effect XOS on growth was assessed vitro. Then, supplemented or not (HFD, 60% energy from fat) (LFD)...
Abstract Physical activity is essential in weight management, improves overall health, and mitigates obesity-related risk markers. Besides inducing changes systemic metabolism, habitual exercise may improve gut’s microbial diversity increase the abundance of beneficial taxa a correlated fashion. Since there lack integrative omics studies on overweight populations, we studied metabolomes gut microbiota associated with programmed obese individuals. We measured serum fecal metabolites 17 adult...
Pre-diabetes and non-alcoholic fatty liver disease (NAFLD) are associated with an unhealthy lifestyle pose extremely high costs to the healthcare system. In this study, we aim explore whether individualized aerobic exercise (AEx) low carbohydrate diet (LCh) intervention affect hepatic fat content (HFC) in pre-diabetes via modification of gut microbiota composition other post-interventional effects. A 6-month randomized follow-up is conducted from January 2013 December 2015. The target sample...
The urea cycle disease carbamoyl-phosphate synthetase deficiency (CPS1D) has been associated with many mutations in the CPS1 gene [Häberle et al., 2011. Hum Mutat 32:579-589]. disease-causing potential of most these is unclear. To test effects, we have developed a system for recombinant expression, mutagenesis, and purification human 1 (CPS1), very large, complex, fastidious enzyme. kinetic molecular properties are essentially same as natural CPS1. Glycerol partially replaces essential...
A common complexity from increasing rates of overweight is metabolic dysfunction associated steatotic liver disease (MASLD) which can develop into more severe conditions such as fibrosis or hepatocellular carcinoma. The gut and its microbial flora interact with the through gut-liver axis supporting healthy composition metabolism microbiota benefit health. We have previously shown in animals that a xylo-oligosaccharide (XOS) prebiotic drive beneficial ameliorate hepatic steatosis- our current...
Background Competitive sports and nutrition, popular among amateur athletes aiming for a lean physique, have limited research on gut microbiota.
Abstract Background Pancreatic cancer (PC) presents a significant challenge in oncology because of its late-stage diagnosis and limited treatment options. The inadequacy current screening methods has prompted investigations into stool-based assays microbial classifiers as potential early detection markers. gut microbiota composition PC patients may be influenced by population differences, thereby impacting the accuracy disease prediction. However, comprehensive profiling analysis these...
Carbamoyl-phosphate synthetase I (CPS1) deficiency (CPS1D), a recessively inherited urea cycle error due to CPS1 gene mutations, causes life-threatening hyperammonemia. The disease-causing potential of missense mutations in can be ascertained with the recombinant expression and purification system reported here, which uses baculovirus insect cells. We study this effects nine clinical one polymorphism on solubility, stability, activity, kinetic parameters for NAG. Five (p.T471N, p.Q678P,...
The aim of this study was to compare 6 weeks short-term moderate intensity aerobic exercise and dieting on serum metabolomics cardio-metabolic risk factors in pre-menopausal women. Ninety previously inactive overweight obese (BMI 25–35 kg/m2) women (age 41.5 ± 7.6 years) were randomized either a 6-week Nordic walking program (EX, n = 45) or dietary counseling group (DI, 45). Body composition, glucose, insulin lipids measured. Serum low-molecular-weight metabolites lipid constituents analyzed...
Accumulating evidence indicates that gut microbiota plays a significant role in obesity, insulin resistance and associated liver disorders. Family Enterobacteriaceae especially Enterobacter cloacae strain B29 have been previously linked to obesity hepatic damage. The underlying mechanisms, however, remain unclear. Therefore, we comprehensively examined the effects of E. subsp. (ATCC® 13047™) administration on host metabolism mice fed with high-fat diet (HFD). C57BL/6N were randomly divided...