A5006610873- SARS-CoV-2 detection and testing
- COVID-19 diagnosis using AI
- Radiation Detection and Scintillator Technologies
- Metabolomics and Mass Spectrometry Studies
- Cancer-related Molecular Pathways
- Energy and Environment Impacts
- Gut microbiota and health
- Diet and metabolism studies
- Phytochemicals and Antioxidant Activities
- Amino Acid Enzymes and Metabolism
- Mycotoxins in Agriculture and Food
- Clusterin in disease pathology
- Diet, Metabolism, and Disease
- Ubiquitin and proteasome pathways
- Nutrition, Genetics, and Disease
- Advanced biosensing and bioanalysis techniques
- RNA modifications and cancer
- Analytical Chemistry and Chromatography
- Microtubule and mitosis dynamics
- Pregnancy and preeclampsia studies
- Cholesterol and Lipid Metabolism
- Edible Oils Quality and Analysis
- Reproductive Biology and Fertility
- Systemic Lupus Erythematosus Research
- Antioxidant Activity and Oxidative Stress
Centre for Omic Sciences
2015-2024
Universitat Rovira i Virgili
2015-2024
Universitat de Barcelona
2002-2024
Technological Center of Nutrition and Health
2017-2022
Wageningen University & Research
2022
Technology Centre Prague
2022
Institut d'Investigació Sanitària Pere Virgili
2020
Consorci Institut D'Investigacions Biomediques August Pi I Sunyer
2002-2011
Consejo Superior de Investigaciones Científicas
2009
International Centre for Genetic Engineering and Biotechnology
2009
Abstract NAD + is a vital redox cofactor and substrate required for activity of various enzyme families, including sirtuins poly(ADP-ribose) polymerases. Supplementation with precursors, such as nicotinamide mononucleotide (NMN) or riboside (NR), protects against metabolic disease, neurodegenerative disorders age-related physiological decline in mammals. Here we show that kinase 1 (NRK1) necessary rate-limiting the use exogenous NR NMN synthesis. Using genetic gain- loss-of-function models,...
The SET protein and the cell cycle inhibitor p21Cip1 interact in vivo vitro. We identified here domain157LIF159 of as essential for binding SET. also found that contains at least two domains interaction with p21Cip1, one located fragment amino acids 81–180 other including 181–277. co-localize nucleus a temporal manner. Overexpression blocks G2/M transition COS HCT116 cells. Moreover, inhibits cyclin B-CDK1 activity both vitro types. This effect is specific these complexes since did not...
Obesity and its comorbidities are currently considered an epidemic, the involved pathophysiology is well studied. Hypercaloric diets tightly related to obesity etiology also cause alterations in gut microbiota functionality. Diet antibiotics known play crucial roles changes ecosystem disruption of balance; therefore, manipulation may represent accurate strategy understand relationship with caused by diet. Fecal transplantation, during which fecal from a healthy donor transplanted obese...
To assess the sustained and acute effects, as well influence of consumption on orange juice (OJ) with a natural hesperidin content hesperidin-enriched OJ (EOJ) blood (BP) pulse (PP) pressures in pre- stage-1 hypertensive individuals.In randomized, parallel, double-blind, placebo-controlled trial, participants (n = 159) received 500 mL/day control drink, OJ, or EOJ for 12 weeks. Two dose-response studies were performed at baseline after weeks.A single dose (500 mL) reduced systolic BP (SBP)...
Preeclampsia is a pregnancy-specific multisystem disorder and leading cause of maternal perinatal morbidity mortality. The exact pathogenesis this multifactorial disease remains poorly defined. We applied proteomics analysis on blood samples collected from 14 singleton pregnancies with early-onset severe preeclampsia 6 uncomplicated to investigate the pathophysiological pathways involved in specific subgroup preeclampsia. Maternal was drawn at diagnosis for cases matched gestational age...
Abstract Background Human viruses released into the environment can be detected and characterized in wastewater. The study of wastewater virome offers a consolidated perspective on circulation within population. Because occurrence severity viral infections vary across person’s lifetime, studying samples contributed by various demographic segments provide valuable insights prevalence these segments. In our study, targeted enrichment sequencing was employed to characterize human at...
The study determines the sustained and acute effects of a red-fleshed apple (RFA), rich in anthocyanins (ACNs), white-fleshed (WFA) without ACNs, an infusion from Aronia melanocarpa (AI) with equivalent content ACNs as RFA, on different cardiometabolic risk biomarkers hypercholesterolemic subjects. A randomized, parallel was performed for 6 weeks two dose-response studies were at baseline after intervention. At weeks, RFA consumption improved ischemic reactive hyperemia decreased C-reactive...
The effects of olive oil phenolic compounds (PCs) on HDL proteome, with respect to new aspects cardioprotective properties, are still unknown. aim this study was assess the impact protein cargo intake virgin (VOO) and two functional VOOs, enriched their own PCs (FVOO) or complemented thyme (FVOOT), in hypercholesterolemic subjects. Eligible volunteers were recruited from IMIM-Hospital del Mar Medical Research Institute (Spain) April 2012 September 2012. Thirty-three participants (total...
Lupin has recently been added to the list of allergens requiring mandatory advisory labeling on foodstuffs sold in European Union, and since December 2008, all products containing even trace amounts lupin must be labeled correctly. globulins consist two major called α-conglutin (11S "legumin-like") β-conglutin (7S "vicilin-like") another additional globulins, γ-conglutin δ-conglutin, which are present lower amounts. We report a methodology facilitate extraction each these proteins using...
Using in vitro selection, high affinity DNA aptamers to the food allergen Lup an 1, ß-conglutin, were selected from a pool of DNA, 93 bases length, containing randomised sequence 49 bases. ß-conglutin was purified lupin flour and chemically crosslinked carboxylated magnetic beads. Peptide mass fingerprinting used confirm presence ß-conglutin. Single stranded generated using T7 Gene 6 Exonuclease subsequently incubated with beads captured released amplified prior further round Systematic...
Abstract Wastewater-based epidemiology has shown to be an efficient tool track the circulation of SARS-CoV-2 in communities assisted by wastewater treatment plants (WWTPs). The challenge comes when this approach is employed help Health authorities their decision-making. Here, we describe roadmap for design and deployment SARSAIGUA, Catalan Surveillance Network Sewage. network monitors, weekly or biweekly, 56 WWTPs evenly distributed across territory serving 6 M inhabitants (80% population)....
Abstract Over the last few years, application of high‐throughput meta‐omics methods has provided great progress in improving knowledge gut ecosystem and linking its biodiversity to host health conditions, offering complementary support classical microbiology. Gut microbiota plays a crucial role relevant diseases such as obesity or cardiovascular disease (CVD), regulation is closely influenced by several factors, dietary composition. In fact, polyphenol‐rich diets are most palatable treatment...
Metabolic syndrome (MetS) is a global epidemic concern. Polyphenols are proposed as good candidates for its prevention, although their mechanisms not fully understood. The gut microbiota seems to play key role in polyphenol beneficial effects. Here, we assessed the effects of citrus hesperidin combining an untargeted metabolomics approach, which has inherent potential elucidate host-microbiome interplay, with extensive anthropometric and biochemical characterizations integrating results our...
Preeclampsia is a multi-system disorder unique to pregnancy responsible for great part of maternal and perinatal morbidity mortality. The precise pathogenesis this complex still unrevealed.We examined the pathophysiological pathways involved in early-onset preeclampsia, specific subgroup representing its most severe presentation, using LC-MS/MS metabolomic analysis based on multi-level extraction lipids small metabolites from blood samples, collected at time diagnosis 14 preeclamptic six...
Overexpression of p21 cip1 induces cell cycle arrest. Although this ability has been correlated with its nuclear localization, the evidence is not conclusive. The mutants that were used to inhibit translocation could no longer bind several proteins known interact last 25 amino acids . Here we point mutation analysis and fusion DsRed identify which are essential for localization We conclude RKR 140–142 While wild‐type DsRed‐p21 arrest in 95% transfected cells, overexpression cytoplasmatic...
The protein-protein complexes formed between different cyclins and cyclin-dependent kinases (CDKs) are central to cell cycle regulation. These represent interesting points of chemical intervention for the development antineoplastic molecules. Here we describe identification an all d-amino acid hexapeptide, termed NBI1, that inhibits kinase activity 2 (cdk2)-cyclin A complex through selective binding cyclin A. mechanism inhibition is non-competitive ATP protein substrates. In contrast...
Cyclin dependent kinases (cdks) regulate cell cycle progression and transcription. We report here that the transcriptional co-activator PCAF directly interacts with cdk2. This interaction is mainly produced during S G2/M phases of cycle. As a consequence this association, inhibits activity cyclin/cdk2 complexes. effect specific for cdk2 because does not inhibit either cyclin D3/cdk6 or B/cdk1 activities. The inhibition neither competitive ATP, nor substrate histone H1 suggesting somehow...
Scope Hydroxytyrosol (HT) is the major phenolic compound in virgin olive oil (VOO) both free and complex forms (secoiridoids; SEC). Proteomics of cardiovascular tissues such as aorta or heart represents a promising tool to uncover mechanisms action compounds healthy animals. Methods results Twelve female Wistar rats were separated into three groups: standard diet two diets supplemented (HT SEC) adjusted 5 mg/kg/day during 21 days. Proteomic analyses performed by nano‐LC MS. Ingenuity Pathway...