A5100404900- RNA modifications and cancer
- Metal-Organic Frameworks: Synthesis and Applications
- Sirtuins and Resveratrol in Medicine
- Advanced oxidation water treatment
- Analytical chemistry methods development
- Atmospheric chemistry and aerosols
- RNA and protein synthesis mechanisms
- Cancer-related molecular mechanisms research
- Molecular Sensors and Ion Detection
- Sulfur Compounds in Biology
- Catalytic Processes in Materials Science
- Advanced biosensing and bioanalysis techniques
- Biofuel production and bioconversion
- Atmospheric Ozone and Climate
- Adsorption and biosorption for pollutant removal
- Environmental remediation with nanomaterials
- Magnetism in coordination complexes
- Calcium signaling and nucleotide metabolism
- Atmospheric and Environmental Gas Dynamics
- Cholesterol and Lipid Metabolism
- Cocoa and Sweet Potato Agronomy
- Arsenic contamination and mitigation
- Analytical Chemistry and Chromatography
- Cancer-related gene regulation
- Advanced Photocatalysis Techniques
Bohai University
2024
Jiangsu University
2022-2024
Dezhou University
2024
Jinshan Hospital of Fudan University
2024
Hebei Agricultural University
2024
Cornell University
2012-2023
Guangdong Ocean University
2021-2023
Ningxia Medical University General Hospital
2023
Ningxia Medical University
2019-2023
Weill Cornell Medicine
2023
Missense mutations in PTEN-induced kinase 1 (PINK1) cause autosomal-recessive inherited Parkinson's disease (PD). We have exploited our recent discovery that recombinant insect PINK1 is catalytically active to test whether directly phosphorylates 15 proteins encoded by PD-associated genes as well reported bind PINK1. discovered efficiently only one of these proteins, namely the E3 ligase Parkin. mapped phosphorylation site a highly conserved residue within Ubl domain Parkin at Ser(65). show...
Here we report a new rhodamine-based fluorescent probe containing selenium−nitrogen bond for detecting thiols based on the nucleophilic substitution of sulfhydryl. The was successfully applied to imaging in both HL-7702 cells and HepG2 with high sensitivity selectivity.
Photolysis of nitric acid on the surface has been found recently to be greatly enhanced from that in gas phase. Yet, photolysis particulate nitrate (pNO3) associated with atmospheric aerosols is still relatively unknown. Here, aerosol filter samples were collected both near ground and throughout troposphere board NSF/NACR C-130 aircraft. The rate constants pNO3 determined these by directly monitoring production rates nitrous (HONO) nitrogen dioxide (NO2) under UV light (>290 nm) irradiation....
Photolysis of nitric acid and nitrate (HNO3/nitrate) was investigated on the surfaces natural artificial materials, including plant leaves, metal sheets, construction materials. The were conditioned in outdoor air prior to experiments receive depositions ambient HNO3/nitrate other atmospheric constituents. photolysis rate constant (JHNO3(s)) surface measured based production rates nitrous (HONO) nitrogen oxides (NOx). JHNO3(s) values, from 6.0 × 10–6 s–1 3.7 10–4 s–1, are 1 3 orders...
Rubisco activase is a nuclear-encoded chloroplast protein that required for the light activation of ribulose 1,5-bisphosphate carboxylase/oxygenase (Rubisco) in vivo . In most plants examined to date, there are two isoforms arising from alternative splicing differ only at carboxyl terminus. Here we demonstrate with recombinant proteins Arabidopsis larger isoform has unique role regulation activity. At physiological ratios ADP/ATP, 46-kDa minimal ATP hydrolysis and activity comparison 43-kDa...
With the recent quick expansion of DNA and protein sequence databases, intensive efforts are underway to interpret linear genetic information in terms function, structure, control biological processes. The systematic identification quantification expressed proteins has proven particularly powerful this regard. Large-scale is usually achieved by automated liquid chromatography-tandem mass spectrometry complex peptide mixtures database searching resulting spectra [Aebersold Goodlett, Chem....
A simple, scalable and reproducible technology that allows direct formation of large numbers homogeneous synchronized embryoid bodies (EBs) defined sizes from dissociated human induced pluripotent stem cells (hiPSCs) was developed. Non-cell-adhesive hydrogels were used to create round-bottom microwells host hiPSCs. No Rho-associated kinase inhibitor (ROCK-i), or centrifugation needed the side effects ROCK-i can be avoided. The key requirement for successful EB in addition non-cell-adhesive...
We report a new spiropyran-based fluorescent probe that exhibits high affinity and specificity towards Aβ oligomers both in vitro vivo. This can penetrate the blood brain barrier specifically target brains of transgenic mice models for Alzheimer's disease.
Distance constraints in proteins and protein complexes provide invaluable information for calculation of 3D structures, identification binding partners localization protein−protein contact sites. We have developed an integrative approach to identify characterize such sites through the analysis proteolytic products derived from chemically cross-linked by isotopically coded cross-linkers using LC-MALDI tandem mass spectrometry computer software. This method is specifically tailored toward...
Two Permian-Triassic boundary (PTB) sections from south China provide insights regarding the origin of negative excursions in carbonate and organic carbon isotope records associated with largest mass extinction Earth history. Xiakou, a ramp section, exhibits delta C-13(carb) up to -2 parts per thousand, Xinmin, deep-shelf C-13(org) -6 thousand. In both sections, these are volcanic ash layers, excursion size scales layer thickness. These relationships document direct influence volcanism on...
Osteosarcoma is the most common primary malignant bone tumor in children and young adults. RNA N6-methyladenosine (m6A) abundant internal modification mammalian mRNA, which involved tumorigenesis progression. It has been reported that methyltransferase-like 3 (METTL3), first m6A "writer", plays critical roles cancer However, its role molecular mechanism osteosarcoma poor studied. In this study, we aimed to investigate functional underlying of METTL3 progression osteosarcoma.We detected mRNA...
Hydrogen sulfide (H2S) is an important cellular signaling molecule, and its physiological pathophysiological properties have been under intensive investigation. In this study, a novel ratiometric fluorescent H2S donor (HSD-B) has developed, which exhibited the following advantages: (i) scavenging ROS producing simultaneously; (ii) providing fluorescence for visualization quantification of releasing; (iii) targeting mitochondrion specifically. Moreover, it demonstrated protective effects on...