A5025161047- Cardiac electrophysiology and arrhythmias
- Ion channel regulation and function
- Cardiac Ischemia and Reperfusion
- Cardiovascular Effects of Exercise
- Cardiac Arrhythmias and Treatments
- Receptor Mechanisms and Signaling
- Cardiomyopathy and Myosin Studies
- Cardiac Fibrosis and Remodeling
- ECG Monitoring and Analysis
- Atrial Fibrillation Management and Outcomes
- Cardiac Arrest and Resuscitation
- Cardiovascular Function and Risk Factors
- Heart Rate Variability and Autonomic Control
- Cardiac pacing and defibrillation studies
- Electrochemical Analysis and Applications
- Neuroscience and Neural Engineering
- Viral Infections and Immunology Research
- Anesthesia and Neurotoxicity Research
- Cardiovascular and exercise physiology
- Sirtuins and Resveratrol in Medicine
- Neuroscience and Neuropharmacology Research
- Nitric Oxide and Endothelin Effects
- Mitochondrial Function and Pathology
- Cardiovascular Disease and Adiposity
- Fuel Cells and Related Materials
University of Szeged
2015-2024
Farmak (Czechia)
2017-2023
MTA-SZTE Research Group on Artificial Intelligence
2020
West German Heart and Vascular Center Essen
2016
Heidelberg University
2015-2016
University Hospital Heidelberg
2015-2016
Universität Hamburg
2016
University of Regensburg
2016
NYU Langone Health
2016
University of Lübeck
2016
Background: Myocardial metabolic impairment is a major feature in chronic heart failure. As the coenzyme fuel oxidation and oxidative phosphorylation substrate for enzymes signaling energy stress response, nicotinamide adenine dinucleotide (NAD + ) emerging as target number of diseases including Little known on mechanisms regulating homeostasis NAD failing heart. Methods: To explore possible alterations heart, we quantified expression biosynthetic human mouse model dilated cardiomyopathy...
Antiarrhythmic management of atrial fibrillation (AF) remains a major clinical challenge. Mechanism-based approaches to AF therapy are sought increase effectiveness and provide individualized patient care. K(2P)3.1 (TASK-1 [tandem P domains in weak inward-rectifying K+ channel-related acid-sensitive channel-1]) 2-pore-domain (K(2P)) channels have been implicated action potential regulation animal models. However, their role the pathophysiology treatment paroxysmal chronic patients with is...
Abstract Aims Atrial fibrillation (AF) is associated with altered cAMP/PKA signaling and an AF-promoting reduction of L-type Ca2+-current (ICa,L), the mechanisms which are poorly understood. Cyclic-nucleotide phosphodiesterases (PDEs) degrade cAMP regulate PKA-dependent phosphorylation key calcium-handling proteins, including ICa,L-carrying Cav1.2α1C subunit. The aim was to assess whether function PDE type-8 (PDE8) isoforms contributes ICa,L in persistent (chronic) AF (cAF) patients. Methods...
Background and purpose: Assessing the proarrhythmic potential of compounds during drug development is essential. However, reliable prediction drug‐induced torsades de pointes arrhythmia (TdP) remains elusive. Along with QT interval prolongation, assessment short‐term variability (STV(QT)) may be a good predictor TdP. We investigated relative importance I Ks Kr block in TdP together correlations between QTc interval, incidence Experimental approach: ECGs were recorded from conscious dogs...
Purpose Aging has severe implications for tissue damage and is a major risk factor disease. However, the effects of aging on cardiac extracellular matrix (ECM) components in individuals free cardiovascular disease are incompletely understood. We aimed at characterization ECM proteins heart men women. Experimental design Left ventricular (LV) samples nondiseased human hearts technically unusable transplantation obtained from general organ donors ( n = 31; age 17–68 years; 48% women) were used...
Atrial fibrillation (AF) prevalence increases with advanced stages of left ventricular (LV) dysfunction. Remote proarrhythmic effects dysfunction on atrial electrophysiology remain incompletely understood. We hypothesized that repolarizing K2P3.1 K+ channels, previously implicated in AF pathophysiology, may contribute to shaping the action potential (AP), forming a specific electrical substrate LV might represent target for personalized antiarrhythmic therapy.A total 175 patients exhibiting...
Aging is associated with increased inflammation and alterations in mitochondrial biogenesis, which promote the development of cardiovascular diseases.Emerging evidence suggests a role for sirtuins, are NAD +dependent deacetylases, regulation biogenesis.Sirtuins regulated by sex or hormones decreased during aging animal models.We hypothesized that age-related cardiac Sirt1 Sirt3 occur human heart examined whether these changes decrease anti-oxidative defense, inflammatory state...
Abstract Linked to exacerbated inflammation, myocarditis is a cardiovascular disease, which may lead dilated cardiomyopathy. Although sex and age differences in the development of chronic have been postulated, underlying cellular mechanisms remain poorly understood. In current study, we aimed investigate mitochondrial homeostasis, senescence. Cardiac tissue samples from younger older patients with inflammatory cardiomyopathy (DCMI) were used. The expression Sirt1, phosphorylated AMPK,...
Protracted QT interval (QTI) adaptation to abrupt heart rate (HR) changes has been identified as a clinical arrhythmic risk marker. This study investigates the ionic mechanisms of QTI and its relationship risk. Computer simulations experimental recordings in human canine ventricular tissue were used investigate basis action potential duration (APD) HR with protocol commonly studies. The time for 90% is 3.5 min simulations, agreement data humans. APD follows similar dynamics, being faster...
In this study the effects of a new, highly selective sodium-calcium exchanger (NCX) inhibitor, ORM-10962 were investigated on cardiac NCX current, Ca2+ transients, cell shortening and in experimental arrhythmias. The level selectivity novel inhibitor several major transmembrane ion currents (L-type repolarizing K+ currents, late Na+ Na+/K+ pump current) was also determined.Ion single dog ventricular cells (cardiac myocytes; CM), action potentials multicellular preparations recorded utilizing...
Abstract Aims Quantitative real-time RT-PCR (RT-qPCR) has become the method of choice for mRNA quantification, but requires an accurate normalization based on use reference genes showing invariant expression across various pathological conditions. Only few data exist appropriate human heart. The objective this study was to determine a set suitable in atrial and ventricular tissues, from right left cavities control cardiac diseases. Methods results We assessed 16 (ACTB, B2M, GAPDH, GUSB,...
In search of more efficacious and safe pharmacological treatments for atrial fibrillation (AF), atria-selective antiarrhythmic agents have been promoted that target ion channels principally expressed in the atria. This concept allows one to engage effects atria, but spares ventricles from potentially proarrhythmic side effects. It has suggested cardiac small conductance Ca 2+ -activated K + (SK) may represent an mammals including humans. However, there are conflicting data concerning...
BACKGROUND: Atrial fibrillation (AF) susceptibility is heightened in endurance athletes but the underlying mechanisms are incompletely understood. Because pulmonary vein (PV) myocyte triggers critical determinants of AF, we investigated PV electrophysiological remodelling animal models athlete's heart. METHODS: The following experiments were performed canines and mice after 16 or 6 weeks, respectively, daily exercise training (ExT), compared to sedentary (Sed) controls: ECG recording,...
The cardiovascular benefits of red wine consumption are often attributed to the antioxidant effects its polyphenolic constituents, including quercetin, catechin and resveratrol. Inhibition cardiac voltage-gated sodium channels (VGSCs) is antiarrhythmic cardioprotective. As polyphenols may also modulate ion channels, possess structural similarities several VGSC inhibitors, we hypothesised that inhibition contribute cardioprotection by these polyphenols.The whole-cell voltage-clamp technique...
In recent years genome-wide association studies (GWAS) have uncovered numerous chromosomal loci associated with various electrocardiographic traits and cardiac arrhythmia predisposition. A considerable fraction of these lie within inter-genic regions. The underlying trait-associated variants likely reside in regulatory regions exert their effect by modulating gene expression. Hence, the key to unraveling molecular mechanisms is interrogate for differential transcript abundance expression...
The reliable assessment of proarrhythmic risk compounds under development remains an elusive goal. Current safety guidelines focus on the effects blocking KCNH2/HERG ion channel-in tissues and animals with intact repolarization. Novel models better predictive value are needed that more closely reflect conditions in patients cardiac remodelling reduced repolarization reserve.We have developed a model for long QT syndrome type-5 rabbits (LQT5 ) cardiac-specific overexpression mutant (G52R)...
The opening of cardiac plasma‐membrane ATP‐sensitive K + channels ( pm ATP ) can protect the heart against ischaemia/reperfusion injury. We recently demonstrated that resting membrane potential E m ventricular myocytes strongly modulates reoxygenation‐induced Ca 2+ overload. This led to hypothesis activation influence extent chemically induced hypoxia (CIH)/reoxygenation overload via hyperpolarization diastolic myocytes. isolated rat was determined using perforated patch‐clamp technique and...