A5026432045- Renal Diseases and Glomerulopathies
- Ion channel regulation and function
- Genetic and Kidney Cyst Diseases
- Ion Transport and Channel Regulation
- Muscle Physiology and Disorders
- Chronic Kidney Disease and Diabetes
- Erythrocyte Function and Pathophysiology
- Monoclonal and Polyclonal Antibodies Research
- Ion Channels and Receptors
- Neutrophil, Myeloperoxidase and Oxidative Mechanisms
- Birth, Development, and Health
- Telomeres, Telomerase, and Senescence
- Lanthanide and Transition Metal Complexes
- Hedgehog Signaling Pathway Studies
- Fetal and Pediatric Neurological Disorders
- Pancreatic function and diabetes
- Connexins and lens biology
- Immunotherapy and Immune Responses
- Cardiac electrophysiology and arrhythmias
- Supramolecular Self-Assembly in Materials
- Mitochondrial Function and Pathology
- Advanced MRI Techniques and Applications
- Circular RNAs in diseases
- Blood properties and coagulation
- Congenital heart defects research
Max Delbrück Center
2015-2025
Vistec Electron Beam (Germany)
2012
The prorenin receptor (PRR) is highly expressed in podocytes, but its role the maintenance of podocyte function unknown. Here we generated podocyte-specific PRR-knockout mice and found that these animals died between 2 to 3 wk after birth. Within 14 d, developed nephrotic syndrome, albuminuria with foot-process fusion, cytoskeletal changes. Podocyte-specific PRR deletion also led disturbed processing multivesicular bodies enrichment autophagosomal (LC3) lysosomal (LAMP2) markers, indicating...
The mitochondrial contact site and cristae organizing system (MICOS) is crucial for the formation of crista junctions inner membrane architecture. MICOS contains two core components. Mic10 shows membrane-bending activity, whereas Mic60 (mitofilin) forms sites between outer membranes. Here we report that deforms liposomes into thin tubules thus displays membrane-shaping activity. We identify a membrane-binding in soluble intermembrane space-exposed part Mic60. This formed by predicted...
Article31 March 2015Open Access Source Data Insm1 cooperates with Neurod1 and Foxa2 to maintain mature pancreatic β-cell function Shiqi Jia Corresponding Author Developmental Biology, Max-Delbrück-Center for Molecular Medicine, Berlin, Germany Search more papers by this author Andranik Ivanov Systems Biology of Gene Regulatory Elements, Dinko Blasevic Thomas Müller Bettina Purfürst Electron Microscopy Platform, Wei Sun Scientific Genomics Chen Matthew N Poy Mechanisms Metabolic Disease,...
Healthy placental development is essential for reproductive success; failure of the feto-maternal interface results in pre-eclampsia and intrauterine growth retardation. We found that grainyhead-like 2 (GRHL2), a CP2-type transcription factor, highly expressed chorionic trophoblast cells, including basal (BCT) cells located at chorioallantoic murine placentas. Placentas from Grhl2-deficient mouse embryos displayed defects BCT cell polarity basement membrane integrity interface, as well...
Recent structural studies show distinct morphologies for the fibrils of Aβ(1-42) and Aβ(1-40), which are believed not to co-fibrillize. We describe here a novel, structurally-uniform 1 : mixed fibrillar species, differs from both pure fibrils. It forms preferentially even when Aβ(1-40) peptides in non-stoichiometric ratio.
Atherosclerosis and vasculitis both feature inflammation mediated by neutrophil-endothelial cell (EC) contact. Neutrophil myeloperoxidase (MPO) can disrupt normal EC function, although the mechanism(s) which MPO is transferred to ECs are unknown. We tested hypothesis that close, β2 integrin-dependent neutrophil-EC contact mediates transfer from neutrophils ECs. used sensitive assays flow cytometry detect in demonstrate acquired when contacted directly but not were separated Transwells. The...
The development of cellular tracking by fluorine (19F) magnetic resonance imaging (MRI) has introduced a number advantages for following immune cell therapies in vivo. These include improved signal selectivity and possibility to correlate cells labeled with fluorine-rich particles conventional anatomic proton (1H) imaging. While the optimization labeling method is clearly important, impact on dynamics should be kept mind. We show 19F MR spectroscopy (MRS) that efficiency murine system...
Background The Muscle-specific RING-finger (MuRF) protein family of E3 ubiquitin ligases is important for maintenance muscular structure and function. MuRF proteins mediate adaptation striated muscles to stress. MuRF2 MuRF3 bind microtubules are implicated in sarcomere formation with noticeable functional redundancy. However, if this redundancy muscle function vivo unknown. Our objective was investigate cooperative the skeletal heart vivo. Methods double knockout mice (DKO) were generated...
Magnetic resonance imaging (MRI) provides the opportunity of tracking cells in vivo. Major challenges dissecting from recipient tissue and signal sensitivity constraints albeit exist. In this study, we aimed to tackle these limitations order study inflammation autoimmune encephalomyelitis. We constructed a very small dual-tunable radio frequency (RF) birdcage probe tailored for (19)F (fluorine) (1)H (proton) MR mouse neuroimaging. The novel design eliminated need extra electrical components...
Unraveling the genetic susceptibility of complex diseases such as chronic kidney disease remains challenging. Here, we used inbred rat models damage associated with elevated blood pressure for comprehensive analysis a major albuminuria locus detected in these models. We characterized its genomic architecture by congenic substitution mapping, targeted next-generation sequencing, and compartment-specific RNA sequencing isolated glomeruli. This led to prioritization transmembrane protein...
Abstract In mammalian peripheral nerves, unmyelinated C‐fibers usually outnumber myelinated A‐fibers. By using transmission electron microscopy, we recently showed that the saphenous nerve of naked mole‐rat ( Heterocephalus glaber ) has a C‐fiber deficit manifested as substantially lower C:A‐fiber ratio compared with other mammals. Here determined uniqueness this by performing quantitative anatomical analysis several nerves in five further members Bathyergidae family: silvery Heliophobius...
ABSTRACT CAR-like membrane protein (CLMP), an immunoglobulin cell adhesion molecule (IgCAM), has been implicated in congenital short-bowel syndrome humans, a condition with high mortality for which there is currently no cure. We therefore studied the function of CLMP Clmp-deficient mouse model. Although we found that levels mRNAs encoding Connexin43 or Connexin45 were not only marginally affected, respectively, by Clmp deficiency, absence caused severe reduction both proteins smooth muscle...
Ahnak1 is a giant, ubiquitously expressed, plasma membrane support protein whose function in skeletal muscle largely unknown. Therefore, we investigated whether ahnak would be influenced by alterations of the sarcolemma exemplified dysferlin mutations known to render vulnerable or calpain3, protease cleave ahnak. Human biopsy specimens obtained from patients with limb girdle muscular dystrophy (LGMD) caused (LGMD2B) and calpain3 (LGMD2A) were for expression localization. We found that ahnak1...
ABSTRACT Pathogenic gene variants in humans that affect the sonic hedgehog (SHH) pathway lead to severe brain malformations with variable penetrance due unknown modifier genes. To identify such modifiers, we established novel congenic mouse models. LRP2-deficient C57BL/6N mice suffer from heart outflow tract defects and holoprosencephaly caused by impaired SHH activity. These are fully rescued on a FVB/N background, indicating strong influence of Applying comparative transcriptomics,...
The membrane protein TMEM150C has been proposed to form a mechanosensitive ion channel that is required for normal proprioceptor function. Here, we examined whether expression of in neuroblastoma cells lacking Piezo1 associated with the appearance currents. Using three different modes mechanical stimuli, indentation, stretch, and substrate deflection, could not evoke currents expressing TMEM150C. We next asked if necessary mechanosensitivity cutaneous sensory neurons. used an available mouse...
Background: Glomerular hyperfiltration (GH) is an important mechanism in the development of albuminuria hypertension. Upregulation COX2 (cyclooxygenase 2) and prostaglandin E 2 (PGE ) was linked to podocyte damage GH. We explored potential renoprotective effects either separate or combined pharmacological blockade EP2 receptor type EP4 4) Methods: conducted vivo studies a transgenic zebrafish model ( Tg[fabp10a:gc-EGFP] suitable for analysis glomerular filtration barrier function genetic rat...
Magnetic resonance (MR) methods to detect and quantify fluorine (19F) nuclei provide the opportunity study fate of cellular transplants in vivo. Cells are typically labeled with 19F nanoparticles, introduced into living organisms tracked by MR methods. Background-free imaging quantification cell numbers amongst strengths MR-based tracking but challenges pertaining signal sensitivity detection exist. In this we aimed overcome these limitations manipulating aminophospholipid composition...