A5058028736- Blood Coagulation and Thrombosis Mechanisms
- Renal Diseases and Glomerulopathies
- Cell Adhesion Molecules Research
- Protease and Inhibitor Mechanisms
- Connective Tissue Growth Factor Research
- Carcinogens and Genotoxicity Assessment
- MicroRNA in disease regulation
- Agricultural safety and regulations
- RNA Research and Splicing
- Advanced Chemical Sensor Technologies
- Cardiac Fibrosis and Remodeling
- Coronary Interventions and Diagnostics
- Antiplatelet Therapy and Cardiovascular Diseases
- Genomics, phytochemicals, and oxidative stress
- Protein Kinase Regulation and GTPase Signaling
- Genetically Modified Organisms Research
- Biomarkers in Disease Mechanisms
- Extracellular vesicles in disease
- Hormonal Regulation and Hypertension
- Chronic Kidney Disease and Diabetes
- Systemic Sclerosis and Related Diseases
- Animal Genetics and Reproduction
- Coagulation, Bradykinin, Polyphosphates, and Angioedema
- Effects and risks of endocrine disrupting chemicals
- Acute Myocardial Infarction Research
Federal Institute for Risk Assessment
2019-2025
Charité - Universitätsmedizin Berlin
2013-2022
Freie Universität Berlin
2019-2022
Humboldt-Universität zu Berlin
2007-2022
Berlin Institute of Health at Charité - Universitätsmedizin Berlin
2019
Max Delbrück Center
2016
Zero to Three
2011
Vlaams Instituut voor Biotechnologie
2010
Maastricht University
2010
Icahn School of Medicine at Mount Sinai
2009
Bacillus amyloliquefaciens FZB42 is a Gram-positive, plant-associated bacterium, which stimulates plant growth and produces secondary metabolites that suppress soil-borne pathogens. Its 3,918-kb genome, containing an estimated 3,693 protein-coding sequences, lacks extended phage insertions, occur ubiquitously in the closely related subtilis 168 genome. The B. genome reveals unexpected potential to produce metabolites, including polyketides bacillaene difficidin. More than 8.5% of devoted...
There is a growing recognition of the importance protein kinases in control alternative splicing. To define underlying regulatory mechanisms, highly selective inhibitors are needed. Here, we report discovery and characterization dichloroindolyl enaminonitrile KH-CB19, potent specific inhibitor CDC2-like kinase isoforms 1 4 (CLK1/CLK4). Cocrystal structures KH-CB19 with CLK1 CLK3 revealed non-ATP mimetic binding mode, conformational changes helix αC phosphate loop halogen bonding to hinge...
We here report on the synthesis, optimization, and biological characterization of leucettines, a family kinase inhibitors derived from marine sponge leucettamine B. Stepwise synthesis analogues starting natural structure, guided by activity testing eight purified kinases, led to highly potent CLKs DYRKs, two families kinases involved in alternative pre-mRNA splicing Alzheimer's disease/Down syndrome. Leucettine L41 was cocrystallized with CLK3. It interacts key residues located within...
Tumor necrosis factor (TNF)-α–stimulated human umbilical vein endothelial cells express 2 naturally occurring forms of tissue (TF), the primary initiator blood coagulation: soluble alternatively spliced isoform and full-length TF isoform. The regulatory pathways enabling this phenomenon are completely unknown. Cdc2-like kinases DNA topoisomerase I regulate alternative splicing via phosphorylation serine/arginine-rich proteins. In study, we examined effects protein on following stimulation...
Alkenylbenzenes occur as natural constituents in a variety of edible plants, particular those herbs and spices used to give distinctive flavor range food feed items. Some alkenylbenzenes with relevance for food, such estragole methyleugenol, are known be genotoxic carcinogenic rodents. However, the potential other structurally related alkenylbenzenes, myristicin elemicin, is still under scientific discussion. Here, we investigated elemicin induce micronuclei (MN) V79 cells comparison that...
Tissue factor (TF) is the primary initiator of blood coagulation. In response to tumor necrosis (TNF)-alpha human umbilical vein endothelial cells (HUVECs) express 2 TF isoforms: a soluble alternatively spliced isoform (asHTF) and membrane-bound "full length" (fl)TF. How differential expression regulated still unknown. This study compared impact PI3K/Akt pathway inhibition on alternative splicing in HUVECs, influence transcriptional regulation by inhibiting nuclear kappa B (NFkappaB).The...
CCN1 is an evolutionary ancient matricellular protein that modulates biological processes associated with tissue repair. Induction at sites of injury was observed in conditions ranging from skin wounds to cardiac diseases, including ischemic and inflammatory cardiomyopathy. Here, we provide evidence a novel function as modulator immune cell migration.to understand the role cardiomyopathies evaluate its therapeutic potential, overexpressed using adenoviral hepatotropic vector murine...
Unraveling the genetic susceptibility of complex diseases such as chronic kidney disease remains challenging. Here, we used inbred rat models damage associated with elevated blood pressure for comprehensive analysis a major albuminuria locus detected in these models. We characterized its genomic architecture by congenic substitution mapping, targeted next-generation sequencing, and compartment-specific RNA sequencing isolated glomeruli. This led to prioritization transmembrane protein...
Angiotensin (Ang)II is involved in induction of proteinuria, renal injury, and apoptosis thus a major contributor to the development chronic kidney disease. Podocytes are importance for pathogenesis several diseases. Decrease podoplanin (PDPN) podocytes podocyte loss has been associated with proteinuria. Little known about regulation biological function PDPN its role AngII-mediated damage. Here, we determined influence AngII on expression PDPN, microRNA (miRNA)-29b miRNA-497 human podocytes....