A5086195155- Acute Kidney Injury Research
- Renal and related cancers
- Chronic Kidney Disease and Diabetes
- Renal Transplantation Outcomes and Treatments
- Genetic and Kidney Cyst Diseases
- Single-cell and spatial transcriptomics
- Organ Transplantation Techniques and Outcomes
- Dialysis and Renal Disease Management
- Birth, Development, and Health
- COVID-19 Clinical Research Studies
- Ferroptosis and cancer prognosis
- Molecular Biology Techniques and Applications
- Pharmacogenetics and Drug Metabolism
- Ion Transport and Channel Regulation
- Pluripotent Stem Cells Research
- Renal and Vascular Pathologies
- IL-33, ST2, and ILC Pathways
- Electrolyte and hormonal disorders
- RNA modifications and cancer
- Asthma and respiratory diseases
- Polyomavirus and related diseases
- Cytomegalovirus and herpesvirus research
- Neurological Complications and Syndromes
- Cannabis and Cannabinoid Research
- Parkinson's Disease Mechanisms and Treatments
Medizinische Hochschule Hannover
2022-2025
Charité - Universitätsmedizin Berlin
2015-2024
Humboldt-Universität zu Berlin
2009-2024
Freie Universität Berlin
2021-2024
Max Delbrück Center
2015-2022
Berlin Institute of Health at Charité - Universitätsmedizin Berlin
2017-2022
Universität Greifswald
2018-2021
NF- κ B is a key regulator of innate and adaptive immunity implicated in the pathogenesis AKI. The cell type–specific functions kidney are unknown; however, pathway serves distinct immune tissue parenchymal cells. We analyzed tubular epithelial-specific signaling mouse model ischemia-reperfusion injury (IRI)–induced reporter activity nuclear localization phosphorylated subunit p65 analyses mice revealed that IRI induced widespread activation renal epithelia interstitial cells peaked 2–3 days...
Two metrics, a rise in serum creatinine concentration and decrease urine output, are considered tantamount to the injury of kidney tubule epithelial cells thereof (AKI). Yet neither criterion emphasizes etiology or pathogenetic heterogeneity acute decreases excretory function. In fact, whether decreased function due contraction extracellular fluid volume (vAKI) intrinsic (iAKI) actually share pathogenesis should be aggregated same diagnostic group remains an open question. To examine this...
Although most nephron segments contain one type of epithelial cell, the collecting ducts consists at least two: intercalated (IC) and principal (PC) cells, which regulate acid-base salt-water homeostasis, respectively. In adult kidneys, these cells are organized in rosettes suggesting functional interactions. Genetic studies mouse revealed that transcription factor Tfcp2l1 coordinates IC PC development. induces expression specific genes, including H+-ATPase subunits Jag1. Jag1 turn,...
Abstract Background Acute kidney injury (AKI) occurs frequently in critically ill patients and is associated with adverse outcomes. Cellular mechanisms underlying AKI cell responses to remain incompletely understood. Methods We performed single-nuclei transcriptomics, bulk molecular imaging studies, conventional histology on tissues from 8 individuals severe (stage 2 or 3 according Kidney Disease: Improving Global Outcomes (KDIGO) criteria). Specimens were obtained within 1–2 h after had...
Severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) utilises the angiotensin-converting enzyme (ACE2) transmembrane peptidase as cellular entry receptor. However, whether SARS-CoV-2 in alveolar compartment is strictly ACE2-dependent and to what extent virus-induced tissue damage and/or direct immune activation determines early pathogenesis still elusive.Spectral microscopy, single-cell/-nucleus RNA sequencing or ACE2 "gain-of-function" experiments were applied infected human lung...
Transcription factor AP-1 is constitutively activated and IRF4 drives growth survival in ALK+ ALK– anaplastic large cell lymphoma (ALCL). Here we demonstrate high-level BATF BATF3 expression ALCL. Both BATFs bind classical motifs interact with ALCL deregulated factors. Together IRF4, they co-occupy AP-1-IRF composite elements, differentiating from non-ALCL. Gene-specific inactivation of BATFs, or global inhibition results retardation and/or death vitro vivo. Furthermore, the AP-1-BATF module...
Grainyhead transcription factors control epithelial barriers, tissue morphogenesis, and differentiation, but their role in the kidney is poorly understood. Here, we report that nephric duct, ureteric bud, collecting duct epithelia express high levels of grainyhead-like homolog 2 (Grhl2) lumen expansion defective Grhl2-deficient mice. In cells, Grhl2 inactivation impaired barrier formation inhibited expansion. Molecular analyses showed GRHL2 acts as a transcriptional activator strongly...
Healthy placental development is essential for reproductive success; failure of the feto-maternal interface results in pre-eclampsia and intrauterine growth retardation. We found that grainyhead-like 2 (GRHL2), a CP2-type transcription factor, highly expressed chorionic trophoblast cells, including basal (BCT) cells located at chorioallantoic murine placentas. Placentas from Grhl2-deficient mouse embryos displayed defects BCT cell polarity basement membrane integrity interface, as well...
Acute kidney injury (AKI) is a major health issue, the outcome of which depends primarily on damage and reparative processes tubular epithelial cells. Mechanisms underlying AKI remain incompletely understood, specific therapies are lacking monitoring course in clinical routine confined to measuring urine output plasma levels filtration markers. Here we demonstrate feasibility potential novel approach assess cellular molecular dynamics by establishing robust urine-to-single cell RNA...
Non-flow periods in fluvial ecosystems are a global phenomenon. Streambed drying and rewetting by sporadic rainfalls could drive considerable changes the microbial communities that govern stream nitrogen (N) availability at different temporal spatial scales. We performed microcosm-based experiment to investigate how dry period duration (DPD) (0, 3, 6, 9 weeks) magnitude of rainfall 4, 21 mm applied end period) affected stocks N riverbed sediments, ammonia-oxidizing bacteria (AOB) archaea...
Calcineurin inhibitors (CNIs) are the backbone for immunosuppression after solid organ transplantation. Although successful in preventing kidney transplant rejection, their nephrotoxic side effects contribute to allograft injury. Renal parenchymal lesions occur cyclosporine A (CsA) as well currently favored tacrolimus (Tac). We aimed study whether chronic CsA and Tac exposures, before reaching irreversible damage, affect renal compartments differentially related pathogenic mechanisms can be...
Antibody-mediated rejection (AMR) remains a major complication after solid organ transplantation (SOT). Current treatment options are inefficient and result in drastic impairment of the general immunity. To selectively eliminate responsible alloreactive B cells characterized by anti-donor-HLA cell receptors (BCRs), we generated T overcoming antibodies (CORA-Ts) engineered with novel chimeric receptor comprising truncated donor-HLA molecule as antigen recognition domain. As proof-of-concept,...
<title>Abstract</title> T-cell mediated rejection (TCMR) remains a significant challenge after kidney transplantation and is associated with reduced allograft outcome. Previous research highlighted the critical role of TCMR-induced renal epithelial injury. Yet, detailed cellular origin these injury responses clinical implications remain poorly understood. To induce acute TCMR, we used mouse models allogeneic (C57BL/6 to BALB/c C57BL/6) syngeneic controls C57BL/6 BALB/c). Molecular changes...
IntroductionAcute kidney injury (AKI) is an important complication in COVID-19, but its precise etiology has not fully been elucidated. Insights into AKI mechanisms may be provided by analyzing the temporal associations of clinical parameters reflecting disease processes and development.MethodsWe performed observational cohort study 223 consecutive COVID-19 patients treated at 3 sites a tertiary care referral center to describe evolvement severe (Kidney Disease: Improving Global Outcomes...
Background Single-cell transcriptomes from dissociated tissues provide insights into cell types and their gene expression may harbor additional information on spatial position the local microenvironment. The kidney’s cells are embedded a gradient of increasing tissue osmolality cortex to medulla, which alter cues for reconstruction. Methods or single-nuclei mRNA sequencing mouse kidneys dissected cortex, outer, inner represent corticomedullary axis, was performed. Computational approaches...
Collecting ducts make up the distal-most tubular segments of kidney, extending from cortex, where they connect to nephron proper, into medulla, release urine renal pelvis. During water deprivation, body preservation is ensured by selective transepithelial reabsorption hypertonic medullary interstitium mediated collecting ducts. The duct epithelium forms tight junctions composed barrier-enforcing claudins and exhibits a higher resistance than other tubule exhibit. However, functional...
Significance Statement Dysregulation of vasopressin-induced water reabsorption in the renal collecting duct leads to diabetes insipidus, a congenital or acquired syndrome. Some forms insipidus lack effective treatments prevent excessive loss hypotonic urine that characterizes condition. The authors previously identified antimycotic drug fluconazole as potential therapy, acting modulate effects channel protein aquaportin-2 (AQP2). In this study, they show vitro and vivo induces...
The anaerobic pathogen Clostridioides difficile is perfectly equipped to survive and persist inside the mammalian intestine. When facing unfavorable conditions C. able form highly resistant endospores. Likewise, biofilms are currently discussed as of persistence. Here a comprehensive proteomics approach was applied investigate molecular processes strain 630Δ erm underlying biofilm formation. comparison proteome from two different forms biofilm-like growth, namely aggregate colonies on agar...
Introduction Even during physiologic aging, the kidney experiences a loss of mass and progressive functional decline. This is clinically relevant as it leads to an increased risk acute chronic disease. The tubular system plays important role in underlying aging process, but involved cellular mechanisms remain largely elusive. Methods Kidneys 3-, 12- 24-month-old male C57BL/6J mice were used for RNA sequencing, histological examination, immunostaining RNA-in-situ-hybridization. Single cell...