A5022789402- Ovarian cancer diagnosis and treatment
- Reproductive Biology and Fertility
- Estrogen and related hormone effects
- Cancer Cells and Metastasis
- Immunotherapy and Immune Responses
- Reproductive System and Pregnancy
- Cancer Immunotherapy and Biomarkers
- RNA Research and Splicing
- Galectins and Cancer Biology
- Neonatal Respiratory Health Research
- interferon and immune responses
- Immune cells in cancer
- Immune Cell Function and Interaction
- Respiratory viral infections research
- Cytokine Signaling Pathways and Interactions
- Renal and related cancers
- Epigenetics and DNA Methylation
- Cancer Genomics and Diagnostics
- Pregnancy and preeclampsia studies
- Cancer-related gene regulation
- Cancer Research and Treatments
- Cancer, Hypoxia, and Metabolism
- Cancer-related Molecular Pathways
- Glycosylation and Glycoproteins Research
- Cervical Cancer and HPV Research
LMU Klinikum
2021-2025
Ludwig-Maximilians-Universität München
1982-2025
German Cancer Research Center
2023-2024
Klinik für Frauenheilkunde
2021-2024
Klinik und Poliklinik für Frauenheilkunde und Geburtshilfe
2021-2024
Deutschen Konsortium für Translationale Krebsforschung
2023-2024
München Klinik
2024
Klinikum rechts der Isar
2023
Humboldt-Universität zu Berlin
2022
Charité - Universitätsmedizin Berlin
2013-2022
Abstract The epithelial lining of the fallopian tube is critical importance for human reproduction and has been implicated as a site origin high-grade serous ovarian cancer. Here we report on establishment long-term, stable 3D organoid cultures from tubes, indicative presence adult stem cells. We show that single cells in vitro can give rise to differentiated organoids containing ciliated secretory Continuous growth differentiation depend both Wnt Notch paracrine signalling. Microarray...
Resource3 February 2020Open Access Source DataTransparent process Stable expansion of high-grade serous ovarian cancer organoids requires a low-Wnt environment Karen Hoffmann Department Molecular Biology, Max Planck Institute for Infection Berlin, Germany Search more papers by this author Hilmar Berger Hagen Kulbe Gynecology, Charité University Medicine, Campus Virchow-Klinikum, Sukanija Thillainadarasan Hans-Joachim Mollenkopf Tomasz Zemojtel BIH Genomics Core Unit, Eliane Taube Pathology,...
Chronic infections of the fallopian tubes with Chlamydia trachomatis (Ctr) cause scarring and can lead to infertility. Here we use human tube organoids genital Ctr serovars D, K E for long-term in vitro analysis. The epithelial monolayer responds active expulsion bacteria into lumen compensatory cellular proliferation-demonstrating a role homeostasis defense against this pathogen. In addition, infection activates LIF signaling, which find be an essential regulator stemness organoids....
Severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) utilises the angiotensin-converting enzyme (ACE2) transmembrane peptidase as cellular entry receptor. However, whether SARS-CoV-2 in alveolar compartment is strictly ACE2-dependent and to what extent virus-induced tissue damage and/or direct immune activation determines early pathogenesis still elusive.Spectral microscopy, single-cell/-nucleus RNA sequencing or ACE2 "gain-of-function" experiments were applied infected human lung...
Abstract Chlamydia , a major human bacterial pathogen, assumes effective strategies to protect infected cells against death-inducing stimuli, thereby ensuring completion of its developmental cycle. Paired with capacity cause extensive host DNA damage, this poses potential risk malignant transformation, consistent circumstantial epidemiological evidence. Here we reveal dramatic depletion p53, tumor suppressor deregulated in many cancers, during infection. Using biochemical approaches and live...
SARS-CoV-2 is the causative agent of immune response-driven disease COVID-19 for which new antiviral and anti-inflammatory treatments are urgently needed to reduce recovery time, risk death long COVID development. Here, we demonstrate that immunoregulatory kinase p38 MAPK activated during viral entry, mediated by spike protein, drives harmful virus-induced inflammatory responses. Using primary human lung explants epithelial organoids, targeting signal transduction with selective clinically...
Background/Objectives: This study aimed to construct a risk score (RS) based on necroptosis-associated genes predict the prognosis of patients with advanced epithelial ovarian cancer (EOC). Methods: EOC data from The Cancer Genome Atlas (TCGA) and Gene Expression Omnibus (GEO) series 140082 (GSE140082) were used. Based known genes, clustering was performed identify molecular subtypes EOC. A least absolute shrinkage selection operator (LASSO)-Cox regression analysis identified key related...
Variable platinum responses drive high mortality in high-grade serous ovarian cancer (HGSOC), but to date, there is no molecular approach define resistance levels for clinical decision-making. Here, we developed the organoid drug assay (ODR-test) with patient-derived organoids from our biobank and found that all HGSOC patients develop under exposure carboplatin, although varying implications. Sustained phenotypic reprogramming cellular plasticity carboplatin pressure emerged as a conserved...
The intracellular human bacterial pathogen Chlamydia trachomatis pursues effective strategies to protect infected cells against death-inducing stimuli. Here, we show that infection evokes 3-phosphoinositide-dependent protein kinase-1 (PDPK1) signaling ensure the completion of its developmental cycle, further leading phosphorylation and stabilization MYC. Using biochemical approaches imaging demonstrate Chlamydia-induced PDPK1-MYC induces host hexokinase II (HKII), which becomes enriched...
While the establishment of an ovarian cancer biobank from patient-derived organoids along with their clinical background information promises advances in research and patient care, standardization remains a challenge due to heterogeneity this lethal malignancy, combined inherent complexity organoid technology. This adaptable protocol provides systematic framework realize full potential considering patient-specific variability progenitors. By implementing structured experimental workflow...
Abstract Mechanisms of epithelial renewal in the alveolar compartment remain incompletely understood. To this end, we aimed to characterize progenitors. Single-cell RNA-sequencing (scRNA-seq) analysis HTII-280 + /EpCAM population from adult human lung revealed subclusters enriched for stem cell signature (ASCS) genes. We found that progenitors organoid culture vitro show phenotypic lineage plasticity as they can yield or bronchial cell-type progeny. The direction differentiation is dependent...
High-grade serous ovarian cancer (HGSOC) intratumoural vasculature evolution remains unknown. The study investigated changes in tumour microvessel density (MVD) a large cohort of paired primary and recurrent HGSOC tissue samples its impact on patients' clinico-pathological outcome.A total 222 (pOC) (rOC) intra-patient were assessed for immunohistochemical expression angiogenesis-associated biomarkers (CD31, to evaluate MVD, VEGF-A). Expression profiles compared between pOCs rOCs correlated...
Abstract Purpose Endometrial cancer (EC) is one of the most common gynaecologic malignancies. Tumor infiltrating regulatory T-cells (Treg) have been reported to a prognostic impact in many Immunotherapeutic strategies are gaining interest for advanced and recurrent EC cases, where treatment options rare. Our study was aimed at determining value Treg progression. Methods specimens from 275 patients 28 controls were screened immunohistochemically presence represented by FoxP3. Correlations...
Gestational diabetes mellitus (GDM) is a growing health concern. Since members of the galectin-family are identified to play role in pathogenesis GDM, we determined galectin-12 as an essential protein due its influence lipolysis and inflammation processes. This study investigates expression placentas women with GDM. The population includes 40 expectant suffering from GDM healthy controls. syncytiotrophoblast (SCT) extra villous trophoblast (EVT) placenta was analyzed by immunohistological...
Abstract Cell surface carbohydrate antigens sialyl Lewis X (sLeX) and Y (LeY) are paramount glycoconjugates abundantly expressed in the receptive endometrium. Furthermore, among important biological functions of both is their role leukocytes adhesion extravasation. Interleukin-1 beta (IL-1β) involved process human embryo implantation placenta development. Here, we used an vitro model to investigate whether sLeX LeY playing a mediated by IL-1β. Our results showing that expression cell was...
The therapy concepts that target several members of krüppel like factor (KLF) family have been achieved in breast cancer (BC). However, the role KLF11 BC remains unclear. This study explored prognostic significance patients and investigated its functional roles this malignancy.Immunohistochemistry (IHC) staining 298 patients' samples was performed to determine KLF11. Then protein level correlated clinicopathological characteristics survival outcomes. Afterward, function vitro with...
We present a protocol for effective biobanking of epithelial ovarian cancer organoids, considering the heterogeneous clinical presentation and high recurrence rates. Our involves parallel testing three media to identify patient-specific optimal conditions. describe steps tissue dissociation, differential seeding, organoid cultivation, biobanking. outline procedures fixation, embedding, staining confocal imaging. Furthermore, we demonstrate that brief cultivation isolates in 2D on plastic...
Because mucinous carcinomas are rare tumors that affect several organ sites and known to originate from different tissues, leading frequent misdiagnoses, the objective was characterize differences between primary of ovary metastatic cancer by studying expression pattern candidate biomarkers.Tissue samples histology were obtained 1985 2015. Individual colon tissue analyzed, including standard (PAX8, CK20, CK7, CDX2, SATB2, estrogen/progesterone) new (MUC1, MUC5AC) biomarkers, which then...