A5026457386- Ovarian cancer diagnosis and treatment
- RNA Research and Splicing
- Estrogen and related hormone effects
- Epigenetics and DNA Methylation
- Endometriosis Research and Treatment
- Endometrial and Cervical Cancer Treatments
- PARP inhibition in cancer therapy
- Autism Spectrum Disorder Research
- Reproductive Biology and Fertility
- Cell death mechanisms and regulation
- Liver physiology and pathology
- Endoplasmic Reticulum Stress and Disease
- Microtubule and mitosis dynamics
- Cancer Cells and Metastasis
- Glycosylation and Glycoproteins Research
- Cancer Research and Treatments
- Cancer-related Molecular Pathways
- Family and Disability Support Research
Ludwig-Maximilians-Universität München
2021-2025
LMU Klinikum
2024-2025
ORCID
2021
Children of older fathers carry an increased risk for developing autism and other disorders. To elucidate the underlying mechanisms, we investigated correlation sperm DNA methylation with paternal age its impact on epigenome offspring. Methylation levels nine candidate genes LINE-1 repeats were quantified by bisulfite pyrosequencing in 162 donors 191 cord blood samples resulting children (conceived IVF/ICSI same samples). Four showed a significant negative between age. For FOXK1 KCNA7,...
Background/Objectives: This study aimed to construct a risk score (RS) based on necroptosis-associated genes predict the prognosis of patients with advanced epithelial ovarian cancer (EOC). Methods: EOC data from The Cancer Genome Atlas (TCGA) and Gene Expression Omnibus (GEO) series 140082 (GSE140082) were used. Based known genes, clustering was performed identify molecular subtypes EOC. A least absolute shrinkage selection operator (LASSO)-Cox regression analysis identified key related...
Variable platinum responses drive high mortality in high-grade serous ovarian cancer (HGSOC), but to date, there is no molecular approach define resistance levels for clinical decision-making. Here, we developed the organoid drug assay (ODR-test) with patient-derived organoids from our biobank and found that all HGSOC patients develop under exposure carboplatin, although varying implications. Sustained phenotypic reprogramming cellular plasticity carboplatin pressure emerged as a conserved...
Platinum-based combination chemotherapy remains the backbone of first-line treatment for patients with advanced epithelial ovarian cancer (EOC). While most initially respond well to treatment, relapse ultimately develop platinum resistance. This study identified FLYWCH-type zinc finger-containing protein 1 (FLYWCH1) as an important regulator in resistance development process. We showed that loss FLYWCH1 promotes EOC cells, and low expression is correlated poor prognosis patients. In...
While the establishment of an ovarian cancer biobank from patient-derived organoids along with their clinical background information promises advances in research and patient care, standardization remains a challenge due to heterogeneity this lethal malignancy, combined inherent complexity organoid technology. This adaptable protocol provides systematic framework realize full potential considering patient-specific variability progenitors. By implementing structured experimental workflow...
Abstract Background For young borderline ovarian tumor (BOT) patients, preservation of the uterus was incorporated as an accepted option into treatment guidelines. endometrioid subtype (eBOT) however, adequate histological evaluation is challenging and might be associated with synchronous endometrial disorders or misinterpreted spread from uterine primaries. Case presentation We report cases two patients eBOT who underwent according to current In both cases, unexpected findings invasive...
While the establishment of an ovarian cancer biobank from patient-derived organoids along with their clinical background information promises advances in research and patient care, standardization remains a challenge due to heterogeneity this lethal malignancy, combined inherent complexity organoid technology. This adaptable protocol provides systematic framework realize full potential considering patient-specific variability progenitors. By implementing structured experimental workflow...
<h3>Introduction/Background</h3> Patient-derived ovarian cancer organoids (PDOs) reflecting functional tumor properties in viable 3D cell cultures, appear to be suitable models study the complex epigenetic landscape of platinum resistance mechanisms high-grade serous (HGSOC). In search for promising potential mediators sensitivity and their effects we searched approaches suppress effector proteins HGSOC PDOs. <h3>Methodology</h3> We assessed morphological, transcriptional changes PDOs after...
<h3>Introduction/Background</h3> Translational approaches with appropriate experimental models are required to understand the mechanisms of platinum resistance in highly variable disease high-grade serous ovarian cancer (HGSOC). Our biobank a cohort more than 200 patient-derived HGSOC organoids (PDOs) enables functional study patient-specific responses and PDO long-term expansion potential after vitro carboplatin exposure by our developed Organoid Forming Efficiency (OFE) Test....
5576 Background: The anti-VEGF antibody bevacizumab (Bev) has become part of the first-line treatment patients with advanced ovarian cancer (OC) due to its significant impact on progression-free survival (PFS). However, a effect overall (OS) was not demonstrated. In this context, we hypothesized that stress-responsive multidrug transporter and modulator neovascularization Ral-binding protein 1 (RalBP1) might be prognostic for patient stratify according their predicted Bev response. Methods:...
High-grade serous ovarian cancer (HGSOC) remains the most lethal gynecological malignancy, and there is still an unmet medical need to deepen basic research on its origins mechanisms of progression. Patient-derived organoids high-grade (HGSOC-PDO) are a powerful model study complexity as they maintain, in vitro, mutational profile cellular architecture tissue. Genetic modifications by lentiviral transduction allow novel insights into signaling pathways potential identification biomarkers...
Abstract Purpose The significance of the non-classical G-protein coupled estrogen receptor (GPER) as positive or negative prognostic factor for ovarian cancer patients remains still controversial. Recent results indicate that an imbalance both co-factors and co-repressors nuclear receptors regulate carcinogenesis by altering transcriptional activity through chromatin remodeling. present study aims to investigate whether expression co-repressor NCOR2 plays a role in GPER signaling which...
The significance of the non-classical G-protein-coupled estrogen receptor (GPER) as positive or negative prognostic factor for ovarian cancer patients remains still controversial. Recent results indicate that an imbalance both co-factors and co-repressors nuclear receptors regulates carcinogenesis by altering transcriptional activity through chromatin remodeling. present study aims to investigate whether expression co-repressor NCOR2 plays a role in GPER signaling which thereby could...
Abstract BackgroundFor young borderline ovarian tumor (BOT) patients, preservation of the uterus was incorporated as an accepted option into treatment guidelines. For endometrioid subtype (eBOT) however, adequate histological evaluation is challenging and might be associated with synchronous endometrial disorders or misinterpreted spread from uterine primaries. Case presentationWe report cases two patients eBOT who underwent according to current In both cases, unexpected findings invasive...