A5005079474- Cancer Cells and Metastasis
- Liver Disease Diagnosis and Treatment
- Helicobacter pylori-related gastroenterology studies
- Lymphoma Diagnosis and Treatment
- Epigenetics and DNA Methylation
- Digestive system and related health
- Liver Diseases and Immunity
- Chronic Lymphocytic Leukemia Research
- Wnt/β-catenin signaling in development and cancer
- Genetic factors in colorectal cancer
- Immune Cell Function and Interaction
- Cancer Genomics and Diagnostics
- RNA modifications and cancer
- Gallbladder and Bile Duct Disorders
- Liver physiology and pathology
- Viral-associated cancers and disorders
- Cervical Cancer and HPV Research
- Galectins and Cancer Biology
- Liver Disease and Transplantation
- Genomics and Chromatin Dynamics
- RNA Research and Splicing
- Cancer Immunotherapy and Biomarkers
- Glioma Diagnosis and Treatment
- Cancer-related gene regulation
- Endoplasmic Reticulum Stress and Disease
Charité - Universitätsmedizin Berlin
2006-2025
Freie Universität Berlin
2024
Humboldt-Universität zu Berlin
2024
Max Planck Institute for Infection Biology
2014-2023
University Hospital Schleswig-Holstein
2021-2023
University of Lübeck
2021-2023
Kiel University
2022-2023
Consorci Institut D'Investigacions Biomediques August Pi I Sunyer
2022
Klinikum rechts der Isar
2012
Leipzig University
2007-2011
The distinction between Burkitt's lymphoma and diffuse large-B-cell is unclear. We used transcriptional genomic profiling to define more precisely distinguish subgroups in other types of mature aggressive B-cell lymphomas.We performed gene-expression using Affymetrix U133A GeneChips with RNA from 220 lymphomas, including a core group 8 lymphomas that met all World Health Organization (WHO) criteria. A molecular signature for was generated, chromosomal abnormalities were detected interphase...
The prognostic value of genetic alterations characteristic glioblastoma in patients treated according to present standards care is unclear.Three hundred one with were prospectively recruited between October 2004 and December 2006 at the clinical centers German Glioma Network. Two fifty-eight had radiotherapy, 199 temozolomide, 189 both, seven another chemotherapy as initial treatment. tumors investigated for TP53 mutation, p53 immunoreactivity, epidermal growth factor receptor,...
Abstract The epithelial lining of the fallopian tube is critical importance for human reproduction and has been implicated as a site origin high-grade serous ovarian cancer. Here we report on establishment long-term, stable 3D organoid cultures from tubes, indicative presence adult stem cells. We show that single cells in vitro can give rise to differentiated organoids containing ciliated secretory Continuous growth differentiation depend both Wnt Notch paracrine signalling. Microarray...
Resource3 February 2020Open Access Source DataTransparent process Stable expansion of high-grade serous ovarian cancer organoids requires a low-Wnt environment Karen Hoffmann Department Molecular Biology, Max Planck Institute for Infection Berlin, Germany Search more papers by this author Hilmar Berger Hagen Kulbe Gynecology, Charité University Medicine, Campus Virchow-Klinikum, Sukanija Thillainadarasan Hans-Joachim Mollenkopf Tomasz Zemojtel BIH Genomics Core Unit, Eliane Taube Pathology,...
<h3>Background and aims</h3> <i>Helicobacter pylori</i> is the causative agent of gastric diseases main risk factor in development adenocarcinoma. In vitro studies with this bacterial pathogen largely rely on use transformed cell lines as infection model. However, approach intrinsically artificial especially inappropriate when it comes to investigating mechanisms cancerogenesis. Moreover, common are often defective crucial signalling pathways relevant cancer. A long-lived primary system...
Abstract Genotoxic colibactin-producing pks + Escherichia coli induce DNA double-strand breaks, mutations, and promote tumor development in mouse models of colorectal cancer (CRC). Colibactin’s distinct mutational signature is reflected human CRC, suggesting a causal link. Here, we investigate its transformation potential using organoids from primary murine colon epithelial cells. Organoids recovered short-term infection with E. show characteristics CRC cells, e.g., enhanced proliferation,...
The progression of chronic liver diseases towards cirrhosis is accompanied by drastic tissue changes. This study combines elaborate transcriptomic and histological methods aiming at spatially resolving the hepatic immune microenvironment in NAFLD (including NASH, primary sclerosing cholangitis, biliary severe alcoholic hepatitis).Human samples were subjected to RNA-sequencing (n=225) imaging cytometry (n=99) across 3 independent patient cohorts. Liver from hepatitis cholangitis patients used...
Abstract The colonic epithelial turnover is driven by crypt-base stem cells that express the R-spondin receptor Lgr5. Signals regulate regeneration upon cell injury are largely unknown. Here, we explore dynamics of Wnt signaling in colon. We identify two populations with active signaling: highly proliferative Lgr5 + /Axin2 cells, as well secretory − cells. Upon depletion, these recruited to contribute crypt regeneration. Chemical induced DSS leads a loss both and Axin2 including...
Objective Helicobacter pylori causes life-long colonisation of the gastric mucosa, leading to chronic inflammation with increased risk cancer. Research on pathogenesis this infection would strongly benefit from an authentic human in vitro model. Design Antrum-derived glands surgery specimens served establish polarised epithelial monolayers via a transient air–liquid interface culture stage study cross-talk H. and adjacent stroma. Results The resulting ‘mucosoid cultures’, so named because...
Chronic infections of the fallopian tubes with Chlamydia trachomatis (Ctr) cause scarring and can lead to infertility. Here we use human tube organoids genital Ctr serovars D, K E for long-term in vitro analysis. The epithelial monolayer responds active expulsion bacteria into lumen compensatory cellular proliferation-demonstrating a role homeostasis defense against this pathogen. In addition, infection activates LIF signaling, which find be an essential regulator stemness organoids....
Abstract The transition zones of the squamous and columnar epithelia constitute hotspots for emergence cancer, often preceded by metaplasia, in which one epithelial type is replaced another. It remains unclear how spatial organization maintained zone niche remodelled during metaplasia. Here we used single-cell RNA sequencing to characterize subpopulations underlying stromal compartment endo- ectocervix, encompassing zone. Mouse lineage tracing, organoid culture single-molecule situ...
Abstract Coinfections with pathogenic microbes continually confront cervical mucosa, yet their implications in pathogenesis remain unclear. Lack of in-vitro models recapitulating epithelium has been a bottleneck to study coinfections. Using patient-derived ectocervical organoids, we systematically modeled individual and coinfection dynamics Human papillomavirus (HPV)16 E6E7 Chlamydia, associated carcinogenesis. The stem cells were genetically manipulated introduce oncogenes mimic HPV16...
Abstract The cellular organization of gastrointestinal crypts is orchestrated by different cells the stromal niche but available in vitro models fail to fully recapitulate interplay between epithelium and stroma. Here, we establish a colon assembloid system comprising diverse cell subtypes. These assembloids development mature resembling vivo diversity organization, including maintenance stem/progenitor compartment base their maturation into secretory/absorptive types. This process supported...
Abstract Purpose: The combined loss of genetic material on chromosomes 1p and 19q is strongly associated with favorable outcome in patients WHO grade 3 anaplastic oligodendroglial tumors. prognostic value 1p/19q 2 tumors less well defined. Importantly, the possible effect has not been studied who were treated radiotherapy or chemotherapy. Experimental Design: Seventy-six oligodendroglioma (n = 33), oligoastrocytoma 30), 6), 7) identified had received chemotherapy after their first operation...
Chlamydia trachomatis (Ctr) causes a range of infectious diseases and is epidemiologically associated with cervical ovarian cancers. To obtain panoramic view Ctr-induced signaling, we performed global phosphoproteomic transcriptomic analyses. We identified numerous Ctr phosphoproteins Ctr-regulated host phosphoproteins. Bioinformatics analysis revealed that these proteins were predominantly related to transcription regulation, cellular growth, proliferation, cytoskeleton organization. In...
Background and Aims: Acute liver failure (ALF) is a rare but life-threatening condition, DILI, particularly acetaminophen toxicity, the leading cause of ALF. Innate immune mechanisms further perpetuate injury, while role adaptive system in DILI-related ALF unclear. Approach Results: We analyzed tissue from 2 independent patient cohorts with identified hepatic T cell infiltration as prominent feature human CD8 + cells were characterized by zonation toward necrotic regions an activated gene...
Abstract Follicular lymphoma (FL) is characterized by a large number of chromosomal aberrations. However, their exact genomic extension and involved target genes remain to be determined. For this purpose, we used array‐based intermediate‐high resolution profiling in combination with Affymetrix™ gene expression analysis. Tumor specimens from 128 FL patients were analyzed for the presence aberrations results correlated clinical data sets mRNA levels. In 114 (89%) cases, total 688 (384...
Abstract Helicobacter pylori causes gastric inflammation, gland hyperplasia and is linked to cancer. Here, we studied the interplay between epithelial stem cells their stromal niche under homeostasis upon H. infection. We find that cell differentiation orchestrated by subsets of either produce BMP inhibitors in base, or ligands at surface. Exposure promotes a feed-forward loop inducing Bmp2 expression themselves, enforcing rapid lineage commitment terminally differentiated mucous pit cells....