A5036304585- Neonatal Respiratory Health Research
- Respiratory viral infections research
- Dermatology and Skin Diseases
- Medical Imaging and Pathology Studies
- Mosquito-borne diseases and control
- Allergic Rhinitis and Sensitization
- CAR-T cell therapy research
- Wound Healing and Treatments
- Vector-Borne Animal Diseases
- Insect symbiosis and bacterial influences
- Contact Dermatitis and Allergies
- Extracellular vesicles in disease
- Inhalation and Respiratory Drug Delivery
- Animal Virus Infections Studies
- Genital Health and Disease
- Hepatitis Viruses Studies and Epidemiology
- Viral Infectious Diseases and Gene Expression in Insects
- Exercise and Physiological Responses
- SARS-CoV-2 and COVID-19 Research
- Hippo pathway signaling and YAP/TAZ
- Skin and Cellular Biology Research
- Biosimilars and Bioanalytical Methods
- Renal and related cancers
- Hair Growth and Disorders
- Vector-borne infectious diseases
Humboldt-Universität zu Berlin
2022-2025
Charité - Universitätsmedizin Berlin
2022-2025
Freie Universität Berlin
2018-2025
German Primate Center
2013
Robert Koch Institute
2010-2013
Bernhard Nocht Institute for Tropical Medicine
2013
Institut de Recherche Agricole pour le Développement
2013
Fundação Oswaldo Cruz
2013
University of Oxford
2010
University of St Andrews
2010
Severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) utilises the angiotensin-converting enzyme (ACE2) transmembrane peptidase as cellular entry receptor. However, whether SARS-CoV-2 in alveolar compartment is strictly ACE2-dependent and to what extent virus-induced tissue damage and/or direct immune activation determines early pathogenesis still elusive.Spectral microscopy, single-cell/-nucleus RNA sequencing or ACE2 "gain-of-function" experiments were applied infected human lung...
Data are missing on the diversity of Plasmodium spp. infecting apes that live in their natural habitat, with limited possibility human-mosquito-ape exchange. We surveyed wild chimpanzees living an undisturbed tropical rainforest habitat and found 5 species: P. malariae, vivax, ovale, reichenowi, gaboni.
<title>Abstract</title> Lung cancer, the leading cause of cancer-related mortality, presents major challenges for both standard-of-care therapies and innovative treatments like CAR T cells due to tumor heterogeneity resistance. To aid in personalized treatment decisions, preclinical models that incorporate these patient-specific factors are essential. In this study, we developed a platform using matched lung tumoroids healthy organoids. The retained identity original tumors, as confirmed by...
Abstract Severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) primarily targets ciliated cells during the initial infection of upper tract. Since uncertainties persist regarding other involved epithelial cell types, we here utilized viral replication analysis, single-cell RNA sequencing, and spectral microscopy on infected air-liquid interface cultures human primary nasal bronchial to discern type proportions in relation SARS-CoV-2 tropism immune activation. We revealed that, next...
Abstract Mechanisms of epithelial renewal in the alveolar compartment remain incompletely understood. To this end, we aimed to characterize progenitors. Single-cell RNA-sequencing (scRNA-seq) analysis HTII-280 + /EpCAM population from adult human lung revealed subclusters enriched for stem cell signature (ASCS) genes. We found that progenitors organoid culture vitro show phenotypic lineage plasticity as they can yield or bronchial cell-type progeny. The direction differentiation is dependent...
Skin equivalents are increasingly used as human-based test systems for basic and preclinical research. Most of the established skin composed primary keratinocytes fibroblasts, isolated either from excised human or juvenile foreskin following circumcisions. Although potential hair follicle-derived cells generation has been shown, this approach normally requires microdissections scalp which there is limited subject compliance ethical approval. In present study, we report a novel method to...
Streptococcus pneumoniae ( S.p. ) is the most common causative agent of community-acquired pneumonia worldwide. A key pathogenic mechanism that exacerbates severity disease disruption alveolar-capillary barrier. However, specific virulence mechanisms responsible for this in human lung are not yet fully understood. In study, we infected living tissue with and observed a significant degradation central junctional proteins occludin VE-cadherin, indicating barrier disruption. Surprisingly,...
Human-based organ models can provide strong predictive value to investigate the tropism, virulence, and replication kinetics of viral pathogens. Currently, such have received widespread attention in study SARS-CoV-2 causing COVID-19 pandemic. Applicable a large set organoid viruses, we step-by-step work instruction for infection human alveolar-like organoids with this protocol collection. We also prepared detailed description on state-of-the-art methodologies assess impact analysis relevant...
This protocol describes the single cell isolation of infected human lung organoids in order to perform RNA sequencing or flow cytometry. The focussed on detailed preparation samples prepare cells for further processing according protocols from 10x Genomics sequencing. described steps also include optional fixation with methanol. In that case, how rehydrate after methanol process them
Organ models have received widespread attention in the study of SARS-CoV-2, pathogen causing current COVID-19 pandemic. Human-based organ can provide strong predictive value to investigate tropism, virulence, and replication kinetics viral pathogens. Applicable a large set organoid viruses, we step-by-step work instruction for infection human lung organoids with SARS-CoV-2 this protocol collection. We also prepared detailed description on state-of-the-art methodologies assess impact analysis...