A5070494503- Genetic factors in colorectal cancer
- Cancer Genomics and Diagnostics
- Colorectal Cancer Treatments and Studies
- Bladder and Urothelial Cancer Treatments
- Cancer Cells and Metastasis
- RNA modifications and cancer
- Cancer-related gene regulation
- Wnt/β-catenin signaling in development and cancer
- Cancer Immunotherapy and Biomarkers
- Cancer-related molecular mechanisms research
- Epigenetics and DNA Methylation
- Urinary and Genital Oncology Studies
- Lung Cancer Treatments and Mutations
- COVID-19 Clinical Research Studies
- Long-Term Effects of COVID-19
- Pancreatic and Hepatic Oncology Research
- Cancer, Lipids, and Metabolism
- Ferroptosis and cancer prognosis
- Gastric Cancer Management and Outcomes
- Lung Cancer Research Studies
- Neuroendocrine Tumor Research Advances
- MicroRNA in disease regulation
- HER2/EGFR in Cancer Research
- Ovarian cancer diagnosis and treatment
- Immune cells in cancer
Charité - Universitätsmedizin Berlin
2018-2025
German Cancer Research Center
2016-2025
Heidelberg University
2016-2025
Humboldt-Universität zu Berlin
2019-2025
Freie Universität Berlin
2019-2025
Deutschen Konsortium für Translationale Krebsforschung
2016-2024
Berlin Institute of Health at Charité - Universitätsmedizin Berlin
2019-2024
Ludwig-Maximilians-Universität München
2012-2023
Helios Hospital Bad Saarow
2022-2023
Orthopädische Universitätsklinik
2023
The newly identified severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) causes COVID-19, a pandemic disease. Moreover, thromboembolic events throughout the body, including in CNS, have been described. Given neurological symptoms observed large majority of individuals with SARS-CoV-2 penetrance CNS is likely. By various means, we demonstrate presence RNA and protein anatomically distinct regions nasopharynx brain. Furthermore, describe morphological changes associated infection such...
Members of the miR-34 family are induced by tumor suppressor p53 and known to inhibit epithelial-to-mesenchymal transition (EMT) therefore presumably suppress early phases metastasis. Here, we determined that exposure human colorectal cancer (CRC) cells cytokine IL-6 activates oncogenic STAT3 transcription factor, which directly represses MIR34A gene via a conserved STAT3-binding site in first intron. Repression was required for IL-6–induced EMT invasion. Furthermore, identified receptor...
Abstract Infection by the new corona virus strain SARS-CoV-2 and its related syndrome COVID-19 has been associated with more than two million deaths worldwide. Patients of higher age preexisting chronic health conditions are at an increased risk fatal disease outcome. However, detailed information on causes death contribution pre-existing to yet is missing, which can be reliably established autopsy only. We performed full body autopsies 26 patients that had died after infection Charité...
Colon cancer cells have previously been demonstrated to contain a subpopulation of CD133+ tumour that the ability initiate growth and are thus referred as colon cancer-initiating or stem (CSCs). As CD133 is currently one best markers characterise CSCs, we analysed in colorectal specimens using immunohistochemistry. We show detection specific antigen localised on glandular-luminal surface cells, whereas undifferentiated at front invasion CD133−. In addition, characterised situ by lack CK20...
Blocking BCL9/β-catenin interaction with a stapled peptide inhibits Wnt-dependent transcription and suppresses growth metastasis in colon cancer multiple myeloma.
Abstract Recent developments in immuno-oncology demonstrate that not only cancer cells, but also the tumor microenvironment can guide precision medicine. A comprehensive and in-depth characterization of is challenging since its cell populations are diverse be important even if scarce. To identify clinically relevant microenvironmental features, we applied single-cell RNA sequencing to ten human lung adenocarcinomas normal control tissues. Our analyses revealed heterogeneous carcinoma...
Microsatellite instability (MSI)/mismatch repair deficiency (dMMR) is a key genetic feature which should be tested in every patient with colorectal cancer (CRC) according to medical guidelines. Artificial intelligence (AI) methods can detect MSI/dMMR directly routine pathology slides, but the test performance has not been systematically investigated predefined thresholds.
CD133, CD44, and CD166 are cell surface markers that have recently been associated with colorectal cancer stem cells. As which of these has the greatest impact on patient prognosis is currently unknown, we compared their expression prognostic significance in 110 adenocarcinomas. We demonstrate CD133 correlates CD166, while both do not correlate CD44. show best sole marker to predict low survival, combined analysis all three may be superior identification low-, intermediate-, high-risk cases cancer.
Background E-cadherin is a major component of adherens junctions. Impaired expression in the small intestine and colon has been linked to disturbed intestinal homeostasis barrier function. Down-regulation associated with pathogenesis infections enteropathogenic bacteria Crohn's disease. Methods Findings To genetically clarify function maintenance epithelial defense line, Cdh1 gene was conditionally inactivated mouse epithelium. Inactivation resulted bloody diarrhea enhanced apoptosis cell...
Abstract In colon cancer, CD133 has recently been used to enrich for a subset of tumour cells with tumour‐initiating capabilities and was therefore suggested mark cancer stem cells. However, this molecule surprisingly shown lack functional importance initiation itself. Herein, we investigated whether may be relevant metastasis in patients, as requires several additional biological characteristics besides initiation, examined the effects knocking down expression cell lines on proliferation,...
The basic helix-loop-helix transcription factor AP4/TFAP4/AP-4 is encoded by a c-MYC target gene and displays up-regulation concomitantly with in colorectal cancer (CRC) numerous other tumor types. Here genome-wide characterization of AP4 DNA binding mRNA expression was performed using combination microarray, chromatin immunoprecipitation, next-generation sequencing, bioinformatic analyses. Thereby, hundreds induced repressed genes were identified. Besides many involved the control...
Abstract Purpose: Here, we determined whether epigenetic inactivation of miR-34a and miR-34b/c genes may serve as a prognostic marker for distant metastases in colon cancer. Experimental Design: Using case–control study design 94 primary cancer samples with without liver metastases, CpG methylation frequencies promoters, expression miR-34a, its targets c-Met, Snail, β-catenin their value. Results: was detected 45.1% (n = 42 93) the strongly associated to (P 0.003) lymph nodes 0.006). 91.9%...
The transcription factor SOX2, which is involved in the induction of pluripotent stem cells and contributes to colorectal carcinogenesis, associated with a poor prognosis colon cancer (CC). Furthermore, SOX2 repressor transcriptional activity β-catenin vitro. Since majority CC develop via an activation Wnt/β-catenin signalling pathway, indicated by nuclear expression β-catenin, we wanted investigate patterns correlate them occurrence lymph node distant metastases as indicators malignant...
Abstract The newly identified severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) causes COVID-19, a pandemic disease presenting with fever, cough, and often pneumonia. Moreover, thromboembolic events throughout the body including central nervous system (CNS) have been described. Given first indication for viral RNA presence in brain cerebrospinal fluid light of neurological symptoms large majority COVID-19 patients, SARS-CoV-2-penetrance CNS is likely. By precisely investigating...
Many infectious diseases are thought to have emerged in humans after the Neolithic revolution. Although it is broadly accepted that this also applies measles, exact date of emergence for disease controversial. We sequenced genome a 1912 measles virus and used selection-aware molecular clock modeling determine divergence rinderpest virus. This represents earliest possible establishment human populations. Our analyses show potentially arose as early sixth century BCE, possibly coinciding with...
Abstract Colorectal cancers (CRC) express the WNT effector protein β-catenin in a heterogeneous subcellular pattern rather than uniformly nucleus. In this study, we investigated important aspect of molecular heterogeneity CRCs by analyzing its basis and relationship with tumor-initiating capability. CRC cells highest levels showed only marginal increase tumor initiation capacity. Notably, high activity correlated coincident activation robust mitogen-activated kinase (MAPK) signaling, which...
Abnormal accumulation and dysregulation of the epidermal growth factor receptor family member HER3 is associated with development various human cancers including those breast, lung, ovary. We have previously shown that in melanoma frequently overexpressed poor prognosis. However, importance colon cancer its putative prognostic significance still unknown.HER3 expression was analyzed primary tumors from 110 patients by immunohistochemistry correlated time progression. Parallel to this,...