A5042942845- SARS-CoV-2 and COVID-19 Research
- Immunotherapy and Immune Responses
- Respiratory viral infections research
- Glycosylation and Glycoproteins Research
- RNA Interference and Gene Delivery
- SARS-CoV-2 detection and testing
- COVID-19 Clinical Research Studies
- Viral gastroenteritis research and epidemiology
- T-cell and B-cell Immunology
- Advancements in Transdermal Drug Delivery
- Fuel Cells and Related Materials
- Advancements in Solid Oxide Fuel Cells
- Extracellular vesicles in disease
- Health and Medical Studies
- Histone Deacetylase Inhibitors Research
- Protein Degradation and Inhibitors
- Galectins and Cancer Biology
- CAR-T cell therapy research
- Clinical practice guidelines implementation
- Male Breast Health Studies
- Brain Metastases and Treatment
- Medical History and Innovations
- Advanced Frequency and Time Standards
- Lightning and Electromagnetic Phenomena
- Escherichia coli research studies
Robert Koch Institute
2019-2024
Pennsylvania State University
2023
National Influenza Center
2022
Max Planck Institute of Colloids and Interfaces
1993-2021
Freie Universität Berlin
2016-2021
Max Planck Society
1993-2015
Bayer (Germany)
2014
Deutsches Herzzentrum der Charité
2006
Ardeypharm (Germany)
2004
Eppendorf (Germany)
1982
Abstract SARS-CoV-2 and its emerging variants of concern remain a major threat for global health. Here we introduce an infection model based upon polarized human Alveolar Epithelial Lentivirus immortalized (hAELVi) cells grown at the air–liquid interface to estimate replication epidemic potential respiratory viruses in lower tract. hAELVI cultures are highly permissive different coronaviruses seasonal influenza A virus upregulate various mediators following infection. Our analysis revealed...
Langerhans cells are a subset of dendritic residing in the epidermis human skin. As such, they key mediators immune regulation and have emerged as prime targets for novel transcutaneous cancer vaccines. Importantly, induction protective T cell immunity by these vaccines requires efficient specific delivery both tumor-associated antigens adjuvants. uniquely express Langerin (CD207), an endocytic C-type lectin receptor. Here, we report discovery specific, glycomimetic ligand employing...
Severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) utilises the angiotensin-converting enzyme (ACE2) transmembrane peptidase as cellular entry receptor. However, whether SARS-CoV-2 in alveolar compartment is strictly ACE2-dependent and to what extent virus-induced tissue damage and/or direct immune activation determines early pathogenesis still elusive.Spectral microscopy, single-cell/-nucleus RNA sequencing or ACE2 "gain-of-function" experiments were applied infected human lung...
The recognition of pathogen surface polysaccharides by glycan-binding proteins is a cornerstone innate host defense. Many members the C-type lectin receptor family serve as pattern receptors facilitating uptake, antigen processing, and immunomodulation. Despite high evolutionary pressure in host-pathogen interactions, it still widely assumed that genetic homology conveys similar specificities. Here, we investigate ligand specificities human murine forms myeloid langerin for simple complex...
Severe acute respiratory syndrome (SARS)-CoV and SARS-CoV-2 infections are characterized by remarkable differences, including infectivity case fatality rate. The underlying mechanisms not well understood, illustrating major knowledge gaps of coronavirus biology. In this study, protein expression the SARS-CoV- SARS-CoV-2-infected human lung epithelial cell line Calu-3 was analyzed using data-independent acquisition-mass spectrometry. This resulted in a comprehensive map infection-related...
Epidemiological data demonstrate that Severe Acute Respiratory Syndrome Coronavirus 2 (SARS-CoV-2) variants of concern (VOCs) Alpha and Delta are more transmissible, infectious, pathogenic than previous variants. Phenotypic properties VOC remain understudied. Here, we provide an extensive functional study replication cell entry phenotypes assisted by reverse genetics, mutational mapping spike in lentiviral pseudotypes, viral cellular gene expression studies, infectivity stability assays...
Bromodomain protein 4 (BRD4), a member of the bromodomain and extra-terminal (BET) family, acts as central element in transcriptional elongation plays essential roles cell proliferation. Inhibition BRD4 binding to acetylated histone tails via its two bromodomains, BD1 BD2, with small-molecule inhibitors has been shown be valid strategy prevent cancer growth. We have evaluated established novel assays that quantify interaction transfected chemical inside cultured cells. Both methods are based...
Dendritic cells (DC) are antigen-presenting coordinating the interplay of innate and adaptive immune response. The endocytic C-type lectin receptors DC-SIGN Langerin display expression profiles restricted to distinct DC subtypes have emerged as prime targets for next-generation immunotherapies anti-infectives. Using heteromultivalent liposomes copresenting mannosides bearing aromatic aglycones with natural glycan ligands, we serendipitously discovered striking cooperativity effects DC-SIGN+...
Fragment-based drug discovery is a powerful complement to conventional high-throughput screening, especially for difficult targets. Screening low-molecular-weight fragments usually requires highly sensitive biophysical methods, because of the generally low affinity identified ligands. Here, we developed cell-based fragment screening assay (cellFy) that allows identification hits in physiologically more relevant environment, contrast isolated target screenings solution. For this,...
The skin is an attractive site for vaccination and harbors a dense network of Langerhans cells that are the prime target antigen delivery approaches in epidermis. While specific targeting has been shown to elicit necessary T-cell response using antibody-based approaches, targeted administration particulate antigens form nanoparticle-based vaccine formulations challenging. We previously reported on ligand human Langerin, C-type lectin expressed cells. This presented liposomes renders them...
Immune modulating therapies and vaccines are in high demand, not least to the recent global spread of SARS-CoV2. To achieve efficient activation immune system, professional antigen presenting cells have proven be key coordinators such responses. Especially targeted approaches, actively directing antigens specialized dendritic cells, promise more effective accompanied by reduced payload due less off-target effects. Although antibody glycan-based targeting receptors on been employed, these...
Summary Background The mRNA vaccine BNT162b2 (Comirnaty, BioNTech/Pfizer) and the candidate CVnCoV (Curevac) each encode a stabilized spike protein of SARS‐CoV2 as antigen but differ with respect to nature (modified versus unmodified nucleotides) amount (30 μg 12 RNA). This study characterizes antisera elicited by these two vaccines in comparison convalescent sera. Methods Sera from vaccinated healthcare workers, sera participants phase I trial 2, 4, 6, 8, or hospitalized patients were...
SARS-CoV-2 utilizes the ACE2 transmembrane peptidase as essential cellular entry receptor. Several studies have suggested abundant expression in human lung, inferring strong permissiveness to infection with resultant alveolar damage and lung injury. Against this expectation, we provide evidence that must be considered scarce, thereby limiting propagation alveolus. Instead, spectral imaging of ex vivo infected lungs COVID-19 autopsy samples depicted macrophages were frequently positive for...
Abstract Background The upper respiratory tract (URT) is the primary entry site for severe acute syndrome 2 (SARS-CoV-2) and other viruses, but its involvement in viral amplification pathogenesis remains incompletely understood. Methods In this study, we investigated nasal epithelial cultures, as well vital explanted tissues, to scrutinize tropism of wild-type SARS-CoV-2 recently emerged B.1.1.7 variant. Results Our analyses revealed a widespread replication competence polarized epithelium...
ABSTRACT Time- and cost-saving surveillance of viral pathogens is achieved by tiled sequencing in which a genome amplified overlapping PCR amplicons qPCR. However, designing pan-specific primers for that have high genomic variability represents major challenge. Here, we present bioinformatics command-line tool, called varVAMP ( var iable v irus amp licons). It relies on multiple sequence alignments highly variable virus sequences enables automatic primer design qPCR or amplicon whole...
Abstract Conventional thin section electron microscopy of viral pathogens, such as the pandemic SARS-CoV-2, can provide structural information on virus particle phenotype and its evolution. We recorded about 900 transmission images different SARS-CoV-2 variants, including Alpha (B.1.1.7), Beta (B.1.351), Delta (B.1.617.2) Omicron BA.2 (B.1.1.529) determined various morphometric parameters, maximal diameter spike number, using a previously published measurement method. The datasets evolved...
Electrocatalysis is a catalytic process where the rate of an electrochemical reaction occurring at electrode-electrolyte interface can be controlled by varying electrical potential. applied to generate hydrogen which stored for future use in fuel cells clean electricity. The solid oxide electrocatalysis specially evolution promising. However, further improvements are essential order meet ever-increasing global energy demand. Improvement performance these high chemical systems directly linked...
Abstract Langerhans cells are a subset of dendritic residing in the epidermis human skin. As such, they key mediators immune regulation and have emerged as prime targets for novel transcutaneous cancer vaccines. Importantly, induction protective T cell immunity by these vaccines requires efficient specific delivery both tumor-associated antigens adjuvants. uniquely express Langerin (CD207), an endocytic C-type lectin receptor. Here, we report discovery specific, glycomimetic ligand employing...
Da es bisher keine allgemeingültige Behandlung des Reizdarmsyndroms (RDS) gibt, wird verstärkt nach Therapiestrategien bei einzelnen Untergruppen RDS gesucht. Aktuelle Befunde liefern Hinweise, wonach Störungen der Darmflora an Pathogenese beteiligt sein können. Daher war Ziel Studie, solche Subgruppen zu definieren, die auf eine Therapie mit dem probiotischen E. coli-Stamm Nissle 1917 (EcN) ansprechen.