A5046871022- Neonatal Respiratory Health Research
- DNA Repair Mechanisms
- Renal and related cancers
- Chronic Kidney Disease and Diabetes
- Inflammatory Bowel Disease
- DNA and Nucleic Acid Chemistry
- Inhalation and Respiratory Drug Delivery
- Acute Kidney Injury Research
- Pulmonary Hypertension Research and Treatments
- Medical Imaging and Pathology Studies
- Extracellular vesicles in disease
- Hemoglobin structure and function
- Carcinogens and Genotoxicity Assessment
- Proteoglycans and glycosaminoglycans research
- COVID-19 Clinical Research Studies
- Interstitial Lung Diseases and Idiopathic Pulmonary Fibrosis
- Immune cells in cancer
- Single-cell and spatial transcriptomics
- PARP inhibition in cancer therapy
- Neuroscience of respiration and sleep
- Autophagy in Disease and Therapy
- Hair Growth and Disorders
- Trauma, Hemostasis, Coagulopathy, Resuscitation
- Inflammatory Myopathies and Dermatomyositis
- Pregnancy-related medical research
Humboldt-Universität zu Berlin
2021-2024
Charité - Universitätsmedizin Berlin
2020-2024
Freie Universität Berlin
2021-2024
Saarland University
2016-2020
Endothelial cell (EC) apoptosis and proliferation of apoptosis-resistant cells is a hallmark pulmonary hypertension (PH). Yet, why some ECs die others proliferate how this contributes to vascular remodeling unclear. We hypothesized that differential response may: (1) relate different EC subsets, namely artery (PAECs) versus microvascular (MVECs); (2) be attributable autophagic activation in both subtypes; (3) cause replacement MVECs by PAECs with subsequent distal vessel muscularization.
Anti-synthetase syndrome (ASyS)-associated myositis is a major subgroup of the idiopathic inflammatory myopathies (IIM) and characterized by disease chronicity with musculoskeletal, dermatological pulmonary manifestations. One eight autoantibodies against aminoacyl-transferase RNA synthetases (ARS) detectable in serum affected patients. However, disease-specific therapeutic approaches have not yet been established.To obtain deeper understanding underlying pathogenesis to identify putative...
Chromosomal instability not only has a negative effect on survival in triple-negative breast cancer, but also the well treatable subgroup of luminal A tumors. This suggests general mechanism independent subtypes. Increased chromosomal (CIN) cancer (TNBC) is attributed to defect DNA repair pathway homologous recombination. Homologous recombination (HR) prevents genomic by and protection replication. It unclear whether genetic alterations actually lead or superior signaling pathways are...
Abstract Crohn’s disease (CD) is an inflammatory bowel (IBD) that can affect any part of the gastrointestinal tract, frequently involving terminal ileum. While colonic mucus alterations in CD patients have been described, ileal and its mechanobiological properties neglected. Our study first kind to decipher viscoelastic network mucus. With aim, oscillatory rheological shear measurements based on airway protocol was thoroughly validated for were performed. pilot analyzed ileum from controls...
Recent investigations analyzed in depth the biochemical and biophysical properties of endothelial glycocalyx. In comparison, this complex cell-covering structure is largely understudied alveolar epithelial cells. To better characterize glycocalyx ultrastructure, unaffected versus injured human lung tissue explants mouse lungs were by transmission electron microscopy. Lung was treated with either heparinase (HEP), known to shed components, or pneumolysin (PLY), exotoxin Streptococcus...
In vitro lung research requires appropriate cell culture models that adequately mimic in vivo structure and function. Previously, researchers have extensively utilized commercially available easily expandable A549 NCI-H441 cells which replicate some yet not all features of alveolar epithelial cells. Specifically, these are often restricted by terminally altered expression while lacking important characteristics. Of late, human primary (hPAEpC) become available, but so far poorly specified....
Abstract The main function of the skin, to protect against environment, is supported by activity different stem cell populations. focus this study was elucidating coping mechanisms cells stimulation constant exposure genotoxic stresses, both endogenous and exogenous, ensure long-term function. Investigation various mouse strains, differing in their DNA repair capacity, enables us clarify fractionated low-dose irradiation (LDR)-induced consequences for populations murine hair follicle (HF)...
The pulmonary epithelial glycocalyx is rich in glycosaminoglycans such as hyaluronan and heparan sulfate. Despite their presence, the importance of these bacterial lung infections remains elusive. To address this, we intranasally inoculated mice with
Abstract The alveolar surface of the lung is lined by an epithelium consisting type I (AECI) and II epithelial cells (AECII). This covered a liquid lining layer (ALL). Besides intra‐alveolar surfactant, ALL also contains glycocalyx on apical side AECI AECII. To better understand glycocalyx, its ultrastructural visualization transmission electron microscopy required. aim this study was to systematically re‐evaluate routine cytochemical methods for specifically glycan components. For purpose,...
Weibel's hypothetical three-dimensional (3-D) model in 1966 provided first ultrastructural details into tubular myelin (TM), a unique, complex surfactant subtype found the hypophase of alveolar lining layer. Although initial descriptions by electron microscopy (EM) were already published 1950s, uniform morphological differentiation from other intra-alveolar subtypes is still missing and potential structure-function relationships remain enigmatic. Technical developments volume EM methods now...
The glycocalyx—a plethora of sugars forming a dense layer that covers the cell membrane—is commonly found on epithelial surface lumen tissue. New glycocalyx specific properties have been defined for various organs in last decade. However, lung alveolar epithelium, its structure and functions remain almost completely unexplored. This is partly due to lack physiologically relevant, cost effective vitro models. As an essential but neglected part barrier, understanding holds promise enhance...
Abstract Uterine rupture during a trial of labor after caesarean delivery (CD) is serious complication for mother and fetus. The lack knowledge on histological features molecular pathways uterine wound healing has hindered research in this area from evolving over time. We analysed collagen content turnover scars histological, ultrastructural level. Therefore, tissue samples the lower segment were obtained CD 16 pregnant women with at least one previous CD, without non‐pregnant premenopausal...
Introduction Acute kidney injury (AKI) is associated with significant morbidity and mortality. The diagnosis currently based on urine output serum creatinine there a lack of biomarkers that directly reflect tubular damage. Here, we establish flow cytometric quantification renal epithelial cells as potential biomarker for quantifying the severity damage predicting AKI outcome. Methods A total 84 patients were included in this study, divided into an exploratory cohort (n=21) confirmatory...
The pulmonary epithelial glycocalyx is rich in glycosaminoglycans such as hyaluronan and heparan sulfate. Despite their presence, the precise role of these bacterial lung infections remains elusive. To address this, we intranasally inoculated mice with Streptococcus pneumoniae presence or absence enzymes targeting sulfate, followed by characterization subsequent disease pathology, inflammation, barrier dysfunction. Enzymatic degradation sulfate exacerbated pneumonia mice, evidenced increased...
The pulmonary epithelial glycocalyx is rich in glycosaminoglycans such as hyaluronan and heparan sulfate. Despite their presence, the precise role of these bacterial lung infections remains elusive. To address this, we intranasally inoculated mice with Streptococcus pneumoniae presence or absence enzymes targeting sulfate, followed by characterization subsequent disease pathology, inflammation, barrier dysfunction. Enzymatic degradation sulfate exacerbated pneumonia mice, evidenced increased...
Zielsetzung: Die Uterusruptur im Rahmen eines vaginalen Geburtsversuchs nach Kaiserschnitt ist auf eine mangelhafte uterine Wundheilung zurückzuführen. Über die molekularbiologischen Ursachen wenig bekannt. Im einer klinisch-experimentellen Studie wurden Kollagengehalt und -expression in Uterusnarben histologischen Schnitten sowie Genexpressionsebene quantifiziert korreliert.