Anna Pascual Reguant
A5071326008- Radiomics and Machine Learning in Medical Imaging
- COVID-19 diagnosis using AI
- Chemical Reactions and Isotopes
- Cancer Research and Treatments
- Radiopharmaceutical Chemistry and Applications
- Single-cell and spatial transcriptomics
- Microfluidic and Capillary Electrophoresis Applications
- COVID-19 Clinical Research Studies
- Immune Cell Function and Interaction
- Immune responses and vaccinations
- IL-33, ST2, and ILC Pathways
- Cancer Cells and Metastasis
- Cell Image Analysis Techniques
- Immunotherapy and Immune Responses
- SARS-CoV-2 and COVID-19 Research
- Eosinophilic Esophagitis
- CAR-T cell therapy research
- Cancer Immunotherapy and Biomarkers
- 3D Printing in Biomedical Research
- Molecular Communication and Nanonetworks
- Cytomegalovirus and herpesvirus research
- Immune cells in cancer
- thermodynamics and calorimetric analyses
- Orthopedic Infections and Treatments
- Systemic Sclerosis and Related Diseases
Centro Nacional de Análisis Genómico
2023-2025
German Rheumatism Research Centre
2017-2024
Charité - Universitätsmedizin Berlin
2017-2024
Humboldt-Universität zu Berlin
2020-2024
Freie Universität Berlin
2020-2024
Leibniz Association
2020-2021
The pathogenesis of severe COVID-19 reflects an inefficient immune reaction to SARS-CoV-2. Here we analyze, at the single cell level, plasmablasts egressed into blood study dynamics adaptive response in patients requiring intensive care. Before seroconversion SARS-CoV-2 spike protein, peripheral display a type 1 interferon-induced gene expression signature; however, following seroconversion, lose this signature, express instead signatures induced by IL-21 and TGF-β, produce mostly IgG1 IgA1....
Palatine tonsils are secondary lymphoid organs (SLOs) representing the first line of immunological defense against inhaled or ingested pathogens. We generated an atlas human tonsil composed >556,000 cells profiled across five different data modalities, including single-cell transcriptome, epigenome, proteome, and immune repertoire sequencing, as well spatial transcriptomics. This census identified 121 cell types states, defined developmental trajectories, enabled understanding functional...
Severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) utilises the angiotensin-converting enzyme (ACE2) transmembrane peptidase as cellular entry receptor. However, whether SARS-CoV-2 in alveolar compartment is strictly ACE2-dependent and to what extent virus-induced tissue damage and/or direct immune activation determines early pathogenesis still elusive.Spectral microscopy, single-cell/-nucleus RNA sequencing or ACE2 "gain-of-function" experiments were applied infected human lung...
Abstract Prolonged lung pathology has been associated with COVID-19, yet the cellular and molecular mechanisms behind this chronic inflammatory disease are poorly understood. In study, we combine advanced imaging spatial transcriptomics to shed light on local immune response in severe COVID-19. We show that activated adventitial niches crucial microenvironments contributing orchestration of prolonged immunopathology. Up-regulation chemokines CCL21 CCL18 associates endothelial-to-mesenchymal...
TH17 cells are major drivers of inflammation and involved in several autoimmune diseases. Tissue is a beneficial host response to infection, but it can also contribute autoimmunity. The crosstalk between tissue the immune system during an inflammatory key for preserving integrity restoring physiological processes. However, how inflamed regulates magnitude by controlling pro-inflammatory T not well characterized so far. Here we show that accumulating small intestine upon express IL-33...
Abstract Innate lymphoid cells (ILCs) emerge in the last few years as important regulators of immune responses and biological processes. Although ILCs are mainly known tissue-resident cells, their precise localization interactions with microenvironment still unclear. Here we combine a multiplexed immunofluorescence technique customized computational, open-source analysis pipeline to unambiguously identify CD127 + situ characterize these microenvironments. Moreover, reveal transcription...
About 15% of patients with common variable immunodeficiency (CVID) develop a small intestinal enteropathy, which resembles celiac disease regard to histopathology but evolves from distinct, poorly defined pathogenesis that has been linked in some cases chronic norovirus (NV) infection. Interferon-driven inflammation is prominent feature CVID it remains unknown how NV infection may contribute. Duodenal biopsies patients, stratified according the presence villous atrophy (VA), IgA plasma cells...
Assessing anti-tumor immune responses and microenvironments in central nervous system (CNS) neoplasms, such as brain tumors leptomeningeal disease (LMD), provides prognostic insights predictive biomarkers. Liquid biopsy of the cerebrospinal fluid (CSF) represents a promising minimally-invasive approach, but its ability to reflect against remains unclear. Here, we used single-cell sequencing CSF cells spatial transcriptomics CNS lesions compare contrast LMD patients with lymphoma (CNSL),...
Abstract Homologous recombination deficiency (HRD) contributes to genomic instability and leads sensitivity poly ADP-ribose polymerase inhibitors (PARPi). HRD also activates the cyclic GMP–AMP synthase (cGAS)-STimulator of INterferon Genes (STING)-Interferon (IFN) pathway, highlighting need understand impact cGAS-STING-IFN signaling on PARPi efficacy. In this study, we analyzed a cohort thirty-five breast cancer (BC) patient-derived xenografts (PDX) mouse-derived allografts (MDA). correlated...
Tissue-resident memory T (TRM) cells are known to be important for the first line of defense in mucosa-associated tissues. However, composition, localization, effector function, and specificity TRM human kidney their relevance renal pathology have not been investigated.Lymphocytes derived from blood, peritumor samples, tumor samples were phenotypically functionally assessed by applying flow cytometry highly advanced histology (multi-epitope ligand cartography) methods.CD69+CD103+CD8+ kidneys...
Abstract The growing number of spatial omic technologies have created a demand for computational tools capable managing, storing, and analyzing datasets with multiple modalities resolutions. Meanwhile, computer vision is becoming an integral part processing data readouts where image registration alignment tissue sections are essential prior to integration. Hence, there need platforms that analyze across diverse resolutions as well those manipulate process images microanatomical structures....
Chronic inflammasome activation in mononuclear phagocytes (MNPs) promotes fibrosis various tissues, including the kidney. The cellular and molecular links between are unclear. To address this question, we fed mice lacking immunological mediators an adenine-enriched diet, which causes crystal precipitation renal tubules, crystal-induced activation, fibrosis. We found that kidney depended on intrarenal inflammasome-dependent type 3 immune response driven by its signature transcription factor...
We introduce Single-Cell Transcriptomics Analysis and Multimodal Profiling (STAMP), a scalable profiling approach of individual cells. Leveraging transcriptomics proteomics imaging platforms, STAMP eliminates sequencing costs, to enable single-cell genomics from hundreds millions cells at an unprecedented low cost. Stamping in suspension onto slides, supports single-modal (RNA or protein) multimodal flexible, ultra-high-throughput formats. allows the analysis single multiple samples within...
Abstract Here we have analyzed the dynamics of adaptive immune response triggered by SARS-CoV-2 in severely affected COVID-19 patients, as reflected activated B cells egressing into blood, at single cell level. Early on, before seroconversion to spike protein, peripheral displayed a type 1 interferon-induced gene expression signature. After seroconversion, lost this signature, expressed IL-21- and TGF-β-induced signatures, mostly IgG1 IgA1. In sustained reaction until day 59, shifted IgA2,...
Abstract Post-acute lung sequelae of COVID-19 are challenging many survivors across the world, yet mechanisms behind poorly understood. Our results delineate an inflammatory cascade events occurring along disease progression within fibrovascular niches. It is initiated by endothelial dysfunction, followed heme scavenging CD163 + macrophages and production CCL18. This chemokine synergizes with local CCL21 upregulation to influence stromal composition favoring mesenchymal transition. The...
Abstract Immune checkpoint inhibition (ICI) has revolutionized oncology, offering extended survival and long-term remission in previously incurable cancers. While highly effective tumors with high mutational burden, lowly mutated cancers, including pediatric malignancies, present low response rate limited predictive biomarkers. Here, we a framework for the identification validation of tumor-reactive T cells as biomarker to quantify ICI efficacy candidates personalized TCR-T cell therapy....
Spatial metabolomics holds great promise for unraveling the complexities of metabolic reprogramming in cancer, yet its development lags behind rapid progress spatial transcriptomics (ST). To bridge this gap and maximize value gene expression datasets, we present Flux Balance Analysis (spFBA), a novel framework that transcends limitations existing set enrichment methods. The spFBA approach builds upon previous work designed bulk single-cell data to return Enrichment Scores, up level single...
Chronic immune activation is a hallmark of human immunodeficiency virus (HIV) infection that significantly impacts disease pathogenesis. However, in-depth studies characterizing the immunological landscape ectocervix during chronic HIV remain scarce despite importance this tissue site for transmission.