A5052802894- T-cell and B-cell Immunology
- Immune Cell Function and Interaction
- Immunotherapy and Immune Responses
- Renal Diseases and Glomerulopathies
- Diabetes and associated disorders
- Multiple Sclerosis Research Studies
- Chronic Kidney Disease and Diabetes
- Eosinophilic Esophagitis
- IL-33, ST2, and ILC Pathways
- Polyomavirus and related diseases
- Neuroinflammation and Neurodegeneration Mechanisms
- CAR-T cell therapy research
- Immune Response and Inflammation
- Liver Disease Diagnosis and Treatment
- Kidney Stones and Urolithiasis Treatments
- Reproductive System and Pregnancy
- Immune cells in cancer
- Endoplasmic Reticulum Stress and Disease
- Gout, Hyperuricemia, Uric Acid
- Cancer, Stress, Anesthesia, and Immune Response
- Muscle and Compartmental Disorders
- Cytokine Signaling Pathways and Interactions
- Ectopic Pregnancy Diagnosis and Management
- Inflammasome and immune disorders
- Biomedical Research and Pathophysiology
Miltenyi Biotec (Germany)
2023-2025
University Hospital Bonn
2015-2024
University of Bonn
2009-2021
Institute of Molecular Biology
2009
University Medical Center of the Johannes Gutenberg University Mainz
2004-2005
Johannes Gutenberg University Mainz
2003-2005
The liver is essential for inducing immunological tolerance toward harmless antigens to maintain immune system homeostasis. However, the precise cellular mechanisms of induction against particle-bound antigens, role local hepatic microenvironment, and implications therapeutic targets in immune-mediated diseases are currently unclear. In order elucidate healthy injured liver, we developed a novel vivo combining systemic delivery low-dose peptide coupled inert particles, readouts,...
The total number of glomeruli is a fundamental parameter kidney function but very difficult to determine using standard methodology. Here, we counted all individual in murine kidneys and sized the capillary tufts by combining vivo fluorescence labeling endothelial cells, novel tissue-clearing technique, lightsheet microscopy, automated registration image analysis. Total hands-on time per organ was <1 hour, counting/sizing finished <3 hours. We also investigated use...
The mechanisms by which regulatory T cells (T regs ) suppress autoantibody production are unclear. Here we have addressed this question using transgenic mice expressing model antigens in the kidney. We report that were essential and sufficient to autoreactive B an antigen-specific manner prevent them from producing autoantibodies. Most of suppression was mediated through inhibitory cell-surface-molecule programmed death-1 (PD-1). Suppression required PD-1 expression on two ligands . ligation...
Background High-dose glucocorticoids are the standard treatment for acute relapses in patients with multiple sclerosis (MS) or neuromyelitis optica spectrum disorder (NMOSD). Therapeutic apheresis can be considered escalation of relapse therapy, but some still do not recover sufficiently. We aimed to explore effects on humoral and cellular immune parameters identify features that correlate beneficial clinical outcomes. Methods studied two cohorts comprising a total 63 MS NMOSD who were...
Commonly described as masters of regulation parasitic helminth infections provide a fascinating insight into the complexity our immune system. As with many other pathogens helminths have developed complex evasion strategies and response host has to find balance between eliciting severe damage eliminate parasite or limiting thereby accepting infection. Nevertheless, one should not forget that these still pose serious public health problem can elicit disfigurement death in individual. An...
CD11b+Gr1+ myeloid derived suppressor cells (MDSC) are known to be very potent suppressors of T cell immunity and can further stratified into granulocytic MDSC monocytic in mice based on expression Ly6G or Ly6C, respectively. Here, using these markers functional assays, we aimed identify whether induced during chronic inflammation leading fibrosis both kidney liver additional could more specifically subsets. In an adenine-induced model inflammation/fibrosis suppressive Ly6Gpos were induced....
Kidney dendritic cells (DCs) regulate nephritogenic T cell responses. Most kidney DCs belong to the CD11b+ subset and promote crescentic GN (cGN). The function of CD103+ subset, which represents <5% DCs, is poorly understood. We studied role in cGN using several lines genetically modified mice that allowed us reduce number these cells. In all lines, we detected a reduction FoxP3+ intrarenal regulatory (Tregs), protect against cGN. Mice lacking transcription factor Batf3 had more profound...
Abstract To study the role of CD25+ regulatory T cells (Tregs) in peripheral B cell tolerance, we generated transgenic rat insulin promoter RIP-OVA/HEL mice expressing model Ags OVA and HEL pancreatic islet β (where RIP is hen egg lysozyme). Adoptively transferred OVA-specific CD4+ CD8+ proliferated only autoantigen-draining lymph node (PLN), demonstrating pancreas-specific Ag expression. Transferred HEL-specific (IgHEL cells) disappeared within 3 wk from but not nontransgenic immunized with...
Although the spleen is a major site where immune tolerance to circulating innocuous antigens occurs, kidney also contributes. Circulating smaller than albumin are constitutively filtered and concentrated in reach renal lymph node by lymphatic drainage, resident dendritic cells (DCs) capture them induce of specific cytotoxic T through unknown mechanisms. Here, we found that coinhibitory cell surface receptor programmed death 1 (PD-1) on mediates their tolerance. Renal DCs CD8(+) XCR1(+)...
Despite remarkable advances in the therapy of multiple sclerosis (MS), patients with MS may still experience relapses. High-dose short-term methylprednisolone (MP) remains standard treatment acute management relapses due to its potent anti-inflammatory and immunosuppressive properties. However, there is a lack studies on cell type-specific transcriptome changes that are induced by this synthetic glucocorticoid (GC). Moreover, it not well understood why some do benefit adequately from MP therapy.
Molecular mechanisms that determine lesion localization or phenotype variation in multiple sclerosis are mostly unidentified. Although transmigration of activated encephalitogenic T cells across the blood-brain barrier (BBB) is a crucial step disease pathogenesis CNS autoimmunity, consequences on brain endothelial integrity upon interaction with such and subsequent formation distribution largely unknown. We made use transgenic spontaneous mouse model autoimmunity characterized by...
Cutaneous dendritic cells (DC) are pivotal for the elicitation of antigen-specific immune responses following gene gun-mediated biolistic transfection skin. We transcriptionally targeted transgene expression to DC using vectors containing murine fascin promoter (pFascin) control antigen production and compared response elicited with conventional DNA immunization plasmid constructs ubiquitously active CMV (pCMV). Biolistic pFascin initiated a marked type 1 characterized by occurrence large...
Abstract To study B‐cell tolerance against non‐lymphoid tissue autoantigens, we generated transgenic rat insulin promoter (RIP)‐OVA/hen egg lysozyme (HEL) mice expressing the model antigens, OVA and HEL, in pancreatic islets. Their vaccination with or HEL induced far less auto‐Ab titers compared non‐transgenic controls. Depletion of CD25 + cells during immunization completely restored production, but did not affect antibodies a foreign control antigen. at later time‐points was effective....
Multiple sclerosis (MS) is a chronic, inflammatory and neurodegenerative disease that leads to irreversible damage the brain spinal cord. The goal of so-called "immune reconstitution therapies" (IRTs) achieve long-term remission by eliminating pathogenic immune repertoire through intense short-term cell depletion. B cells are major targets for effective immunotherapy in MS.The aim this study was analyze gene expression pattern before during IRT (i.e., B-cell depletion after repopulation)...
Chronic inflammasome activation in mononuclear phagocytes (MNPs) promotes fibrosis various tissues, including the kidney. The cellular and molecular links between are unclear. To address this question, we fed mice lacking immunological mediators an adenine-enriched diet, which causes crystal precipitation renal tubules, crystal-induced activation, fibrosis. We found that kidney depended on intrarenal inflammasome-dependent type 3 immune response driven by its signature transcription factor...
Abstract Regulatory T cells (Tregs) maintain self‐tolerance and prevent autoimmunity by controlling autoreactive cells. We recently demonstrated in vivo that Tregs can directly suppress auto‐reactive B via programmed death ligand 1 ( PD ‐L1) ligated ‐1 on caused them to undergo apoptosis. Here, we asked whether this mechanism is utilized thymus‐derived natural and/or peripheral lymphoid tissue‐induced Tregs. first antigen‐specific ‐L1‐expressing were induced the draining lymph node of...