A5031323586- Single-cell and spatial transcriptomics
- Pancreatic and Hepatic Oncology Research
- Cancer Genomics and Diagnostics
- Cancer Cells and Metastasis
- Cancer Immunotherapy and Biomarkers
- Molecular Biology Techniques and Applications
- Glioma Diagnosis and Treatment
- Endometriosis Research and Treatment
- Reproductive System and Pregnancy
- Epigenetics and DNA Methylation
- Immune cells in cancer
- Pluripotent Stem Cells Research
- Immunotherapy and Immune Responses
- Ferroptosis and cancer prognosis
- Nerve injury and regeneration
- Radiomics and Machine Learning in Medical Imaging
- Endometrial and Cervical Cancer Treatments
- Cancer Research and Treatments
- CAR-T cell therapy research
- Bladder and Urothelial Cancer Treatments
- Health, Environment, Cognitive Aging
- Inflammatory mediators and NSAID effects
- Nuclear Receptors and Signaling
- Phagocytosis and Immune Regulation
- Cell Image Analysis Techniques
Jackson Laboratory
2017-2024
UConn Health
2024
Université Paris-Est Créteil
2024
Assistance Publique – Hôpitaux de Paris
2024
Genome Institute of Singapore
2016-2022
University of Connecticut
2019-2020
Centro de Biología Molecular Severo Ochoa
2009-2012
Agency for Science, Technology and Research
2011-2012
Universidad Autónoma de Madrid
2009-2012
Consejo Superior de Investigaciones Científicas
2010
Cancer-associated fibroblasts (CAF) are major players in the progression and drug resistance of pancreatic ductal adenocarcinoma (PDAC). CAFs constitute a diverse cell population consisting several recently described subtypes, although extent CAF heterogeneity has remained undefined. Here we use single-cell RNA sequencing to thoroughly characterize neoplastic tumor microenvironment content human mouse PDAC tumors. We corroborate presence myofibroblastic inflammatory define their unique gene...
Endometriosis negatively impacts the health-related quality of life 190 million women worldwide. Novel advances in nonhormonal treatments for this debilitating condition are desperately needed. Macrophages play a vital role pathophysiology endometriosis and represent promising therapeutic target. In current study, we revealed full transcriptomic complexity endometriosis-associated macrophage subpopulations using single-cell analyses preclinical mouse model experimental endometriosis. We have...
ABSTRACT. Lead exposure is a known cause of hypertension. Although most studies have focused on lead-induced endothelial dysfunction and the involvement reactive oxygen species (ROS), it has been recently demonstrated that vascular wall lead-exposed rats both an altered endothelium-independent relaxing response reduced expression soluble guanylate cyclase (sGC). The aim present study was to determine in vitro incubated rat isolated aortic segments if lead downregulates sGC expression,...
Abstract Three-dimensional (3D) organoid cultures are flexible systems to interrogate cellular growth, morphology, multicellular spatial architecture, and interactions in response treatment. However, computational methods for analysis of 3D organoids with sufficiently high-throughput resolution needed. Here we report Cellos, an accurate, pipeline segmentation using classical algorithms nuclear a trained Stardist-3D convolutional neural network. To evaluate analyze ~100,000 ~2.35 million...
Abstract Endometriosis negatively impacts the health-related quality of life 190 million women worldwide. Novel advances in non-hormonal treatments for this debilitating condition are desperately needed. Macrophages play a vital role pathophysiology endometriosis and represent promising therapeutic target. In current study, we revealed full transcriptomic complexity endometriosis-associated macrophage subpopulations using single-cell analyses preclinical mouse model experimental...
Human neural stem cells derived from the ventral mesencephalon (VM) are powerful research tools and candidates for cell therapies in Parkinson disease. Previous studies with VM dopaminergic neuron (DAn) precursors indicated poor growth potential unstable phenotypical properties. Using model line hVM1 (human mesencephalic 1; a new human fetal line), we have found that Bcl-X(L) enhances generation of DAn cells. Mechanistically, not only exerts expected antiapoptotic effect but also induces...
The comeasurement of both genomic and transcriptomic signatures in single cells is fundamental importance to accurately assess how the genetic information correlates with phenotype. However, existing technologies have low throughput laborious work flows.We developed a new method for concurrent sequencing transcriptome targeted regions (CORTAD-seq) within same cell on an automated microfluidic platform. was compatible downstream library preparation, allowing easy integration into...
Patients with locally advanced and metastatic urothelial carcinoma have a low survival rate (median 15.7 months, 13.1–17.8), only 23% response to monotherapy treatment anti-PDL1 checkpoint immunotherapy. To identify new therapeutic targets, we profiled the immune regulatory signatures during murine cancer development using BBN carcinogen identified an increase in expression of T cell inhibitory protein B7-H4 (VTCN1, B7S1, B7X). temporally correlated decreased lymphocyte infiltration. While...
Neural stem cells constitute a promising source of for transplantation in Parkinson's disease, but protocol controlled dopaminergic differentiation is not yet available. Here we investigated the effect anti-apoptotic protein Bcl-x(L) and oxygen tension on survival human ventral mesencephalic cell line (hVM1). hVM1 over-expressing subline (hVMbcl-x(L)) were differentiated by sequential treatment with fibroblast growth factor-8, forskolin, sonic hedgehog, glial line-derived neurotrophic...
The use of organoid models in biomedical research has grown substantially since their inception. As they gain popularity among scientists seeking more complex and biologically relevant systems, there is a direct need to expand clarify potential uses such systems diverse experimental contexts. Herein we outline high-content screening (HCS) platform that allows researchers screen drugs or other compounds against three-dimensional (3D) cell culture multi-well format (384-well). Furthermore,...
Xenium In Situ Gene Expression assay from 10X Genomics enables multiplexed imaging of RNA transcripts in situ. There are several antibody based technologies that enable protein detection. Hematoxylin and Eosin (H&E) staining is a gold standard method for observing tissue morphology. order to generate highly multimodal dataset, we developed protocol prepare slides on the Phenocycler-Fusion platform H&E following run. Although includes decrosslinking step which akin antigen retrieval,...
ABSTRACT Outcomes for adult patients with a high-grade glioma continue to be dismal and new treatment paradigms are urgently needed. To optimize the opportunity discovery, we performed phase 0/1 dose-escalation clinical trial that investigated tumor pharmacokinetics, pharmacodynamics, single nucleus transcriptomics following combined ribociclib (CDK4/6 inhibitor) everolimus (mTOR in recurrent glioma. Patients (n = 24) harboring 1) CDKN2A / B deletion or CDK4 6 amplification, 2) PTEN loss...
Abstract Endometriosis is characterized by growth of endometrial-like tissue outside the uterus affecting many women in their reproductive age, causing years pelvic pain and potential infertility. Its pathophysiology remains largely unknown, limiting diagnosis treatment. We peritoneal ovarian lesions at single-cell transcriptome resolution compared to matched eutopic endometrium, control organoids derived from these tissues, generating data on over 100,000 cells across 12 individuals....