A5049038086- Neurogenesis and neuroplasticity mechanisms
- Pluripotent Stem Cells Research
- Nerve injury and regeneration
- Neuroscience and Neuropharmacology Research
- Neuroscience and Neural Engineering
- Nuclear Receptors and Signaling
- 3D Printing in Biomedical Research
- Mitochondrial Function and Pathology
- Ion channel regulation and function
- Alzheimer's disease research and treatments
- Cholinesterase and Neurodegenerative Diseases
- CRISPR and Genetic Engineering
- RNA Interference and Gene Delivery
- Genetics and Neurodevelopmental Disorders
- Mesenchymal stem cell research
- Neuroinflammation and Neurodegeneration Mechanisms
- Graphene and Nanomaterials Applications
- Memory and Neural Mechanisms
- Photoreceptor and optogenetics research
- Anesthesia and Neurotoxicity Research
- Autophagy in Disease and Therapy
- Epigenetics and DNA Methylation
- Lipid Membrane Structure and Behavior
- Parkinson's Disease Mechanisms and Treatments
- Neurological disorders and treatments
Universidad Autónoma de Madrid
2014-2024
Centro de Biología Molecular Severo Ochoa
2014-2024
Consejo Superior de Investigaciones Científicas
1992-2018
Lund University
1995-1998
Wallenberg Wood Science Center
1995
University of Castilla-La Mancha
1991
Endocannabinoids are neuromodulators that act as retrograde synaptic messengers inhibiting the release of different neurotransmitters in cerebral areas such hippocampus, cortex, and striatum. However, little is known about other roles endocannabinoid system brain. In present work we provide substantial evidence anandamide (AEA) regulates neuronal differentiation both culture vivo. Thus AEA, through CB1 receptor, inhibited cortical neuron progenitor to mature phenotype. addition, human neural...
Huntington's disease is a progressive neurodegenerative that affects the striatum, above all, GABAergic striatal projection neurons. In present study, we have explored use of genetically modified neural stem cell lines producing nerve growth factor (NGF) or brain-derived neurotrophic (BDNF) as means to protect neurons against excitotoxic damage after transplantation 1 week before injection quinolinic acid into same area. One month lesion, degeneration, lesion size, and loss DARPP-32-positive...
Human brain tissue models such as cerebral organoids are essential tools for developmental and biomedical research. Current methods to generate often utilize Matrigel an external scaffold provide structure biologically relevant signals. however is a nonspecific hydrogel of mouse tumor origin does not represent the complexity protein environment. In this study, we investigated application decellularized adult porcine extracellular matrix (B-ECM) which could be processed into (B-ECM hydrogel)...
Human in vitro models of neural tissue with tunable microenvironment and defined spatial arrangement are needed to facilitate studies brain development disease. Towards this end, embedded printing inside granular gels holds great promise as it allows precise patterning extremely soft constructs. However, support formulations restricted only a handful materials. Therefore, there has been need for novel materials that take advantage versatile biomimicry bulk hydrogels while providing...
A CNS-derived conditionally immortalized temperature-sensitive neural progenitor (CINP) cell line was used to generate NGF-secreting cells suitable for intracerebral transplantation. The were transduced by repeated retroviral infection, using a vector containing the mouse NGF cDNA under control of LTR promoter. Subcloning at permissive temperature (33 degrees C) identified highly clone (NGF-CINP), which contained multiple copies transgene and released rate 2 ng/hr/10(5) in vitro, both 33 37...
Structurally patterned pyrolysed three‐dimensional carbon scaffolds (p3D‐carbon) are fabricated and applied for differentiation of human neural stem cells (hNSCs) developed cell replacement therapy sensing released dopamine. In the absence factors (DF) material induces spontaneous hNSC into mature dopamine‐producing neurons 3D‐topography promotes neurite elongation. presence DF, ≈73–82% hNSCs obtain dopaminergic properties on carbon, a to‐date unseen efficiency in both two‐dimensional (2D)...
Early diagnosis in Alzheimer's disease (AD), prior to the appearance of marked clinical symptoms, is critical prevent irreversible neuronal damage and neural malfunction that lead dementia death. Therefore, there an urgent need generate new contrast agents which reveal by a noninvasive method presence some pathological signs AD. In present study, we demonstrate for first time nanoconjugate composed magnetic nanoparticles bound antiferritin antibody, has been developed based on existence iron...
Compartmentalized microfluidic platforms are an invaluable tool in neuroscience research. However, harnessing the full potential of this technology remains hindered by lack a simple fabrication approach for creation intricate device architectures with high-aspect ratio features. Here, hybrid additive manufacturing is presented open-well compartmentalized neural devices that provides larger freedom design, removes need manual postprocessing, and allows increase biocompatibility system....
Object. Immortalized neural progenitor cells derived from embryonic rat hippocampus (HiB5), were transduced ex vivo with the gene for mouse nerve growth factor (NGF) to secrete NGF (NGF-HiB5) at 2 ng/hr/10 5 in culture. Methods. Fifty-nine male Wistar rats weighing 300 370 g each anesthetized 60 mg/kg sodium pentobarbital and subjected lateral fluid-percussion brain injury of moderate severity (2.3–2.4 atm, 34 rats) or sham (25 rats). At 24 hours postinjury, µl (150,000 cells/µl) [ 3...
Nerve growth factor (NGF) stimulates functional recovery from cognitive impairments associated with aging, either when administered as a purified protein or by means of gene transfer to the basal forebrain. Because procedures need be tested in long-term experimental paradigms assess their vivo efficiency, we have used ex therapy provide local delivery NGF aged rat brain over period 2.5 months transplanting immortalized central nervous system-derived neural stem cells genetically engineered...
A simple method to measure cytosolic calcium binding in intact presynaptic nerve terminals (synaptosomes) from rat brain, which is based on the simultaneous determination of [Ca2']i and total [46Ca2+] quina-loaded synaptosomes undergoing a switch high-to low-calcium containing medium, presented.Binding compartment alone was obtained following depletion storing organelles presence carbonyl cyanide p-trifluoromethoxyphenylhydrazone/oligomycin plus caffeine.Synaptosomes, as compared various...
Transplantation of genetically engineered cells into the CNS offers immense potential for treatment several neurological disorders. Monitoring expression levels transgenes and following changes in cell function distribution over time is critical assessing therapeutic efficacy such vivo . We have lentiviral vectors bearing fusions between different combinations fluorescent bioluminescent marker proteins used bioluminescence imaging intravital-scanning microscopy real to study fate human...
Microglia have recently been established as key regulators of brain development. However, their role in neuronal subtype specification remains largely unknown. Using three different co-culture setups, we show that microglia-secreted factors enhance dopaminergic differentiation somatic and induced pluripotent stem cell-derived human neural cells (NSCs). The effect was consistent across NSC microglial cell lines independent prior activation, although restricted to microglia embryonic origin....
The objective of the present study was to explore whether grafted immortalized neural stem cells, genetically modified secrete nerve growth factor (NGF), can ameliorate neuronal death in adult rat striatum following transient middle cerebral artery occlusion (MCAO). One week after cell implantation striatum, animals were subjected 30 min MCAO. Striatal damage evaluated at cellular level 48 h recirculation using immunocytochemical and stereological techniques. ischaemic insult caused an...