A5047704678- 3D Printing in Biomedical Research
- Innovative Microfluidic and Catalytic Techniques Innovation
- Additive Manufacturing and 3D Printing Technologies
- Pickering emulsions and particle stabilization
- Osteoarthritis Treatment and Mechanisms
- Electrohydrodynamics and Fluid Dynamics
- Fluid Dynamics and Heat Transfer
- Surface Modification and Superhydrophobicity
- Cellular Mechanics and Interactions
- Tissue Engineering and Regenerative Medicine
- Advanced Theoretical and Applied Studies in Material Sciences and Geometry
- Cell Image Analysis Techniques
- Nanomaterials and Printing Technologies
- Electrospun Nanofibers in Biomedical Applications
- Anodic Oxide Films and Nanostructures
- Biotin and Related Studies
- Proteins in Food Systems
- Polymer Surface Interaction Studies
- Graphene and Nanomaterials Applications
- Connective tissue disorders research
- Neuroscience and Neural Engineering
- Bone Tissue Engineering Materials
- Pluripotent Stem Cells Research
- Electrochemical Analysis and Applications
- Microfluidic and Capillary Electrophoresis Applications
Brigham and Women's Hospital
2020-2024
Harvard University
2020-2024
University of Twente
2014-2024
Micronit Microfluidics (Netherlands)
2023
ORCID
2021
Bioengineering Center
2014-2020
Developing biomimetic cartilaginous tissues that support locomotion while maintaining chondrogenic behavior is a major challenge in the tissue engineering field. Specifically, locomotive forces demand with strong mechanical properties, chondrogenesis requires soft microenvironment. To address this challenge, 3D cartilage-like bioprinted using two biomaterials different properties: hard biomaterial to reflect macromechanical properties of native cartilage, and create end, (MPa order...
Modular bioinks based on single cell microgels within distinct injectable prepolymers enable uncoupling of biomaterials' micro- and macroenvironments. These inks allow biofabrication 3D constructs that recapitulate the multiscale modular design native tissues with a resolution. This approach represents major step forward in endowing engineered multifunctionality underlies behavior tissues.
Microfluidic chips provide unparalleled control over droplets and jets, which have advanced all natural sciences. However, microfluidic applications could be vastly expanded by increasing the per-channel throughput directly exploiting output of for rapid additive manufacturing. We unlock these features with in-air microfluidics, a new chip-free platform to manipulate microscale liquid streams in air. By controlling composition impact microjets surface tension-driven encapsulation, we...
Human in vitro models of neural tissue with tunable microenvironment and defined spatial arrangement are needed to facilitate studies brain development disease. Towards this end, embedded printing inside granular gels holds great promise as it allows precise patterning extremely soft constructs. However, support formulations restricted only a handful materials. Therefore, there has been need for novel materials that take advantage versatile biomimicry bulk hydrogels while providing...
Compartmentalized Janus microparticles advance many applications ranging from chemical synthesis to consumer electronics. Although these particles can be accurately manufactured using microfluidic droplet generators, the per-nozzle throughputs are relatively low (∼μL/min). Here, we use "in-air microfluidics" combine liquid microjets in midair, thereby enabling orders of magnitude faster production (∼mL/min) as compared chip-based microfluidics. Monodisperse with diameters between 50 and 500...
Single-cell-laden microgels support physiological 3D culture conditions while enabling straightforward handling and high-resolution readouts of individual cells. However, their widespread adoption for long-term cultures is limited by cell escape. In this work, it demonstrated that escape predisposed to off-center encapsulated High-speed microscopy reveals cells are positioned at the microgel precursor droplets' oil/water interface within milliseconds after droplet formation. conventional...
Abstract Engineered living microtissues such as cellular spheroids and organoids have enormous potential for the study regeneration of tissues organs. Microtissues are typically engineered via self‐assembly adherent cells into spheroids, which characterized by little to no cell–material interactions. Consequently, 3D microtissue models currently lack structural biomechanical biochemical control over their internal microenvironment resulting in suboptimal functional performance limited stem...
Organoids are engineered 3D miniature tissues that defined by their organ-like structures, which drive a fundamental understanding of human development. However, current organoid generation methods associated with low production throughputs and poor control over size function including due to merging, limits clinical industrial translation. Here, we present microfluidic platform for the mass lumenogenic embryoid bodies functional cardiospheres. Specifically, apply triple-jet in-air...
Recapitulating inherent heterogeneity and complex microarchitectures within confined print volumes for developing implantable constructs that could maintain their structure
This work combines computational fluid dynamics simulations and stereolithographic printing to fabricate 3D-parallelized microfluidic droplet generators with equal flow profiles.
Abstract Microcapsules with a liquid core and solid shell composed of hydrophobic nanoparticles are broadly applied in food, pharmaceutics, biotechnologies. For example, Pickering emulsions, colloidosomes, or antibubbles (droplets surrounded by air layers water) enable controlled release active agents, biocompatibility, contact‐less transportation. However, producing nanoparticle‐ polymer‐laden shells at scale is highly challenging, since bulk methods polydisperse microfluidic chips prone to...
Abstract 3D cellular spheroids offer more biomimetic microenvironments than conventional 2D cell culture technologies, which has proven value for many tissue engineering applications. Despite beneficiary effects of culture, clinical translation spheroid is challenged by limited scalability current formation methods. Although recent adoption droplet microfluidics can provide a continuous production process, use oils and surfactants, generally low throughput, requirement additional...
Abstract Spatiotemporal control over engineered tissues is highly desirable for various biomedical applications as it emulates the dynamic behavior of natural tissues. Current spatiotemporal biomaterial functionalization approaches are based on cytotoxic, technically challenging, or non-scalable chemistries, which has hampered their widespread usage. Here we report a strategy to spatiotemporally functionalize (bio)materials competitive supramolecular complexation avidin and biotin analogs....
Abstract Recent advances in optical coding, drug delivery, diagnostics, tissue engineering, shear‐induced gelation, and functionally engineered rheology crucially depend on microparticles microfibers with tunable shape, size, composition. However, scalable manufacturing of the required complex micromaterials remains a long‐standing challenge. Here in‐air polymerization liquid jets is demonstrated as novel platform to produce composition at high throughput (>100 mL h −1 per nozzle). The...
Abstract Cell–matrix interactions govern cell behavior and tissue function by facilitating transduction of biomechanical cues. Engineered tissues often incorporate these employing cell‐adhesive materials. However, using constitutively active materials impedes control over fate elicits inflammatory responses upon implantation. Here, an alternative cell–material interaction strategy that provides mechanotransducive properties via discrete inducible on‐cell crosslinking (DOCKING) materials,...
In situ gelation of water-in-oil polymer emulsions is a key method to produce hydrogel particles. Although this approach in principle ideal for encapsulating bioactive components such as cells, the oil phase can interfere with straightforward presentation crosslinker molecules. Several approaches have been developed induce in-emulsion by exploiting triggered generation or release However, these methods typically rely on photo- acid-based reactions that are detrimental cell survival and...
Cell-laden hydrogel microcapsules enable the high-throughput production of cell aggregates, which are relevant for three-dimensional tissue engineering and drug screening applications. However, current microcapsule strategies limited by their throughput, multistep protocols, amount compatible biomaterials. We here present a single-step process controlled microfluidic single-core using enzymatic outside-in cross-linking tyramine-conjugated polymers. It was hypothesized that physically,...
Living microtissues are used in a multitude of applications as they more closely resemble native tissue physiology, compared to 2D cultures. Microtissues typically composed combination cells and materials varying combinations, which dictated by the applications' design requirements. Their range wide, from fundamental biological research such differentiation studies industrial cruelty-free meat production. However, their translation clinical settings has been hindered due lack scalability...
Microfluidic manufacturing platforms have advanced the production of monodisperse, shape-controlled, and chemically defined micromaterials. However, conventional microfabrication are typically designed fabricated as single-purpose single-use tools, which limits their efficiency, versatility, overall potential. We here present an on-the-fly exchangeable nozzle concept that operates in a transparent, 3D, reusable microfluidic device produced without cleanroom technology. The facile exchange...
Microparticles Producing microparticles with an aqueous core and a controlled nanoparticle- or polymer-laden hydrophobic shell at scale is highly challenging, since bulk methods are polydisperse microfluidic chips prone to clogging slow. In article number 2208894, Jieke Jiang, Claas Willem Visser, co-workers developed platform, called "jetting through liquid layer" (JetALL), produce Pickering emulsions, microcapsules, even antibubbles high-throughput.
Abstract Mechanotransduction is widely used to guide cell fate in hydrogels. Traditionally, hydrogels contain adhesive ligands that dynamically bond with cells stimulate biochemical signaling axes such as YAP‐TAZ. However, the molecular toolbox achieve mechanotransduction remains virtually limited non‐covalent bonds, which limits ability program engineered living matter. Here, it demonstrated on‐cell chemistry can be leveraged covalently tether biomaterials directly onto cells, reveal via...
Abstract Engineering of biomimetic tissue implants provides an opportunity for repairing volumetric muscle loss (VML), beyond a tissue’s innate repair capacity. Here, we present thick, suturable, and pre-vascularized 3D containing human induced pluripotent stem cell-derived myogenic precursor cells (hiPSC-MPCs), which can differentiate into skeletal while maintaining self-renewing pool. The formation contractile myotubes millimeter-long fibers from hiPSC-MPCs is achieved in chemically,...