A5017959231- Osteoarthritis Treatment and Mechanisms
- Hydrogels: synthesis, properties, applications
- 3D Printing in Biomedical Research
- Silk-based biomaterials and applications
- Conducting polymers and applications
- Dendrimers and Hyperbranched Polymers
- Supramolecular Self-Assembly in Materials
- Silicone and Siloxane Chemistry
- Mesenchymal stem cell research
- Advanced Sensor and Energy Harvesting Materials
- Polymer Nanocomposites and Properties
- Chemical Synthesis and Reactions
- Polymer Foaming and Composites
- Polymer Surface Interaction Studies
- Nanomaterials for catalytic reactions
- Innovative Microfluidic and Catalytic Techniques Innovation
- Fuel Cells and Related Materials
- Carbon dioxide utilization in catalysis
- Synthesis and properties of polymers
- Analytical Chemistry and Sensors
- Electrospun Nanofibers in Biomedical Applications
- Proteoglycans and glycosaminoglycans research
- Monoclonal and Polyclonal Antibodies Research
- Supramolecular Chemistry and Complexes
- Pickering emulsions and particle stabilization
University of Twente
2015-2025
Bioengineering Center
2019
Institute of Nanotechnology
2014-2016
Institute of Polymers
2012-2016
Centro de Tecnologia da Informação Renato Archer
2014
A combination of the viscoelastic properties hyaluronic acid (HA) and elastic star shaped 8-arm poly(ethylene glycol) (8-arm PEG) was used to design in-situ forming hydrogels. Hydrogels were prepared by enzymatic crosslinking a partially tyramine modified PEG conjugated HA using horseradish peroxidase in presence hydrogen peroxide. homopolymer conjugates mixtures thereof rapidly formed within seconds under physiological conditions at low polymer enzyme concentrations. Elastic hydrogels with...
Supramolecular and dynamic biomaterials hold promise to recapitulate the time-dependent properties stimuli-responsiveness of native extracellular matrix (ECM). Host–guest chemistry is one most widely studied supramolecular bonds, yet binding characteristics host–guest complexes (β-CD/adamantane) in relevant have mostly focused on singular interactions or nondiscrete multivalent pendent polymers. The stepwise synergistic effect for formation remains relatively unreported. In this work, we...
This article reviews injectable hydrogels and their unique properties such as the ability to fill irregular defect sites support cellular activities. Injectable have become invaluable tools for creating three-dimensional (3D) cell-laden scaffolds in cartilage tissue engineering (CTE). review centers on design requirements clinical applications of this domain. We begin by examining material selection, emphasizing importance biocompatibility mechanical crucial successful CTE. Various...
A new class of redox active hydrogels composed poly(ferrocenylsilane) polyanion and poly(ethylene glycol) chains was assembled, using a copper-free azide-alkyne Huisgen cycloaddition reaction. These organometallic displayed reversible collapse reswelling upon chemical oxidation reduction, respectively, formed relatively well-defined, unaggregated Pd(0) nanoparticles (8.2 ± 2.2 nm) from K2PdCl4 salts.
Three-dimensional (3D) bioprinting has emerged as an important technique for fabricating tissue constructs with precise structural and compositional control. However, developing suitable bioinks biocompatible crosslinking mechanisms remains a significant challenge. This study investigates extrusion-based (EBB) using uniaxial or coaxial nozzles enzymatic (EC) to produce 3D in vitro. Initially, low-molecular-weight dextran-tyramine hyaluronic acid-tyramine (LMW Dex-TA/HA-TA) bioink prepolymers...
The ideal scaffold for cartilage regeneration is expected to provide adequate mechanical strength, controlled degradability, adhesion, and integration with the surrounding native tissue. As it does this, mimics natural ECMs functions, which allow nutrient diffusion promote cell survival differentiation. Injectable hydrogels based on tyramine (TA)-functionalized hyaluronic acid (HA) dextran (Dex) are a promising approach regeneration. properties of used in this study were adjusted by varying...
Osteoarthritis (OA) is a degenerative disease of the joints for which no curative treatment exists. Intra-articular injection stem cells explored as regenerative approach, but rapid clearance from site limits therapeutic outcome. Microencapsulation mesenchymal (MSCs) can extend retention time MSCs, outcomes few studies currently performed are conflicting. We hypothesize that composition micromaterial's shell plays deciding factor in outcome intra-articular MSC injection. To this end, we...
In situ gelation of water-in-oil polymer emulsions is a key method to produce hydrogel particles. Although this approach in principle ideal for encapsulating bioactive components such as cells, the oil phase can interfere with straightforward presentation crosslinker molecules. Several approaches have been developed induce in-emulsion by exploiting triggered generation or release However, these methods typically rely on photo- acid-based reactions that are detrimental cell survival and...
Crown ether units incorporated in the poly(arylene ketone) (PAEK) main chain enhance miscibility of PAEK with sulfonated poly(ether (SPEEK). The resulting blend membranes enable separation monovalent ions.
This study introduces the concept of ion selective actuation in polymer metal composite actuators, employing crown ether bearing aromatic polyether materials. For this purpose, sulfonated poly(arylene ketone) (SPAEK) and containing SPAEK with molar masses suitable for membrane preparation are synthesized. The synthesized polymers characterized using Nuclear magnetic resonance (NMR) fourier transform infrared spectroscopy (FTIR) spectroscopy, thermal gravimetric analysis (TGA), differential...
Hydrogels are widely used in tissue engineering due to their ability form three-dimensional (3D) structures that support cellular functions and mimic the extracellular matrix (ECM). Despite advantages, dextran-based hydrogels lack intrinsic biological activity, limiting use this field. Here, we present a strategy for developing bioactive through sequential thiol-maleimide bio-functionalization enzyme-catalyzed crosslinking. The hydrogel network is formed reaction of tyramine moieties...
ABSTRACT Poly(arylene ether ketone)s (PAEKs) are the most commonly known high‐performance materials used for ion exchange and fuel cell membranes. Described here is design of novel sulfonated PAEKs (SPAEKs) nonsulfonated containing crown units in main chain, which can be sensing applications ion‐selective To this end, 4,4′(5′)‐di(hydroxybenzo)‐18‐crown‐6 was synthesized as monomer a step growth polymerization to form ether‐containing SPAEKs. The successful synthesis 18‐crown‐6 sulfonate...
Polycationic poly(ferrocenylsilane)s (PFS) with tunable amounts of PEG side chains were used for the condensation DNA into polyplexes 110 nm in 5.0 mM HEPES.
Injectable hydrogels are promising materials for cartilage regeneration in tissue engineering due to their tunable crosslinking rates, mechanical properties, and biodegradation profiles. This study investigates the chondrogenic potential of hyaluronic acid (HA) crosslinked via tyramine (TA) moieties, with without gelatin modified TA (Gel-TA). Incorporating Gel-TA improved cell viability, spreading, matrix deposition, particularly medium high molecular weight (MMW HMW) HA-TA/Gel-TA hydrogels....
Previously, 5% w/v hyaluronic acid-tyramine (HA-TA) and dextran-tyramine (Dex-TA) enzymatically cross-linked hybrid hydrogels were demonstrated to provide a mechanically stable environment, maintain cell viability, promote cartilaginous-specific matrix deposition in vitro. In this study, combined with human mesenchymal stem cells (hMSCs), bovine chondrocytes (bCHs), or combination of both 4:1 ratio subcutaneously implanted the backs male female nude rats assess performance cell-laden tissue...