A5009811033- Osteoarthritis Treatment and Mechanisms
- Inflammatory mediators and NSAID effects
- Interstitial Lung Diseases and Idiopathic Pulmonary Fibrosis
- Bone and Joint Diseases
- Veterinary Equine Medical Research
- Immune Response and Inflammation
- Knee injuries and reconstruction techniques
- Mesenchymal stem cell research
- Tendon Structure and Treatment
- Total Knee Arthroplasty Outcomes
- 3D Printing in Biomedical Research
- Lower Extremity Biomechanics and Pathologies
- Cytokine Signaling Pathways and Interactions
- Peroxisome Proliferator-Activated Receptors
- Adipokines, Inflammation, and Metabolic Diseases
- Bone Metabolism and Diseases
- HIV-related health complications and treatments
- Extracellular vesicles in disease
- Angiogenesis and VEGF in Cancer
- Probiotics and Fermented Foods
- Gut microbiota and health
- Retinal Diseases and Treatments
- Genetics and Physical Performance
- Chemokine receptors and signaling
- Studies on Chitinases and Chitosanases
Leiden University Medical Center
2024
University Medical Center Utrecht
2012-2023
Utrecht University
2010-2023
Leiden University
2021-2022
Heidelberg University
2016-2021
University Hospital Heidelberg
2016-2021
St. Antonius Ziekenhuis
2010-2020
Erasmus MC
2012-2015
Abstract Background Mesenchymal stromal/stem cells (MSCs) and MSC-derived extracellular vesicles (MSC-EVs) hold promise as a disease modifying treatment in osteoarthritis (OA). Obesity, its associated inflammation, contribute to OA development metabolic represents specific significant group of the patient population. Given their immunomodulatory properties, MSC MSC-EVs are especially interesting for this patients therapeutic option. Here, we were first compare efficacy MSCs mild model taking...
Summary Idiopathic pulmonary fibrosis (IPF) is a rapidly progressive interstitial lung disease of unknown aetiology. Interleukin (IL)-1β plays an important role in inflammation and has been associated with fibrotic remodelling. We investigated the balance between IL-1β IL-1 receptor antagonist (IL-1Ra) bronchoalveolar lavage fluid (BALF) serum as well influence genetic variability IL1B IL1RN gene on susceptibility cytokine levels. In 77 IPF patients 349 healthy controls, single nucleotide...
Triamcinolone acetonide (TA) is used for osteoarthritis management to reduce pain, and pre-clinical studies have shown that TA limits osteophyte formation. Osteophyte formation known be facilitated by synovial macrophage activation. injections might influence activation subsequently osteophytosis. Although widely applied in clinical care, the mechanism through which exerts this effect remains unknown. In animal study, we investigated vivo effects of on activation, development joint...
Idiopathic pulmonary fibrosis (IPF) is a devastating and progressive lung disease. Its aetiology thought to involve damage the epithelium abnormal repair. Alveolar epithelial cells near areas of remodelling show an increased expression proapoptotic molecules. Therefore, we investigated role genes involved in cell cycle control IPF. Genotypes for five single nucleotide polymorphisms (SNPs) tumour protein 53 (TP53) gene four SNPs cyclin-dependent kinase inhibitor 1A (CDKN1A), encoding p21,...
<i>Background:</i> Intestinal bacteria and intestinal epithelial cells (IEC) may modulate the mucosal immune response. In this study, modulation by <i>Lactobacillus GG (LGG)</i> <i>Bifidobacterium breve (Bb1, Bb2)</i> in presence or absence of IEC was addressed an vitro transwell co-culture model. <i>Methods:</i> UV-killed <i>LGG,</i><i>Bb1, Bb2 </i>or Toll-like receptor (TLR) 2 nucleotide oligomerization domain (NOD)...
Antimalarials chloroquine (CQ) and hydroxychloroquine (HCQ) are widely used as antiinflammatory drugs, but side effects include retinopathy vision loss. The objective of this study was to examine the effect CQ HCQ on barrier integrity retinal pigment epithelial (RPE) cell monolayers in vitro. Permeability ARPE-19 determined using Fluorescein isothiocyanate (FITC)-labeled dextran. influence death expression tight junction molecules examined. significantly increased monolayer permeability...
Cellular senescence, that is, the withdrawal from cell cycle, combined with acquirement of senescence associated secretory phenotype has important roles during health and disease is essential for tissue remodeling embryonic development. Osteoclasts are multinucleated cells, responsible bone resorption, cycle arrest osteoclastogenesis well recognized. Therefore, aim this study was to investigate whether these cells should be considered senescent assess influence hypoxia on their potential...
Summary Background Nutraceuticals are often used in the management of equine osteoarthritis, but scientific evidence their efficacy is lacking. Objectives To study preventive effects two new nutraceuticals after experimental induction synovitis comparison with positive and negative control treatments. Study design Blinded, controlled, randomised experiment. Methods Twenty‐four healthy Standardbred horses were randomly allocated to supplement AT (multi‐ingredient, 28 days), HP (collagen...
Pulmonary fibrosis is defined by an overgrowth of fibroblasts and extracellular matrix deposition, results in respiratory dysfunction that often fatal. It the end stage many chronic inflammatory interstitial lung diseases (ILD) such as sarcoidosis idiopathic pulmonary (IPF). The myeloid-related proteins (MRPs) belong to S100 family calcium-binding are highly expressed neutrophils, macrophages epithelial cells during inflammation. MRP14 stimulates fibroblast proliferation vitro granulomas...
<h3>Background/aims</h3> Tumour necrosis factor-α (TNF-α) is a key mediator of ocular inflammation and its interaction with the retinal pigment epithelium (RPE) may be driving force in vitreoretinal disorders such as age-related macular degeneration, proliferative vitreoretinopathy (PVR) diabetic retinopathy. Under inflammatory conditions, ability RPE cells to maintain blood–retinal barrier immune privilege lost proliferation facilitated. To gain insight into effects TNF-α on cells, gene...
Idiopathic pulmonary fibrosis (IPF) is a progressive fibrotic disease, characterized by fibroblast proliferation and extracellular matrix deposition. CC-chemokine ligand 18 (CCL18) upregulates the production of collagen lung fibroblasts associated with mortality. This study was designed to evaluate influence single nucleotide polymorphisms (SNPs) in CCL18 gene on expression survival IPF. Serum levels four SNPs were analyzed 77 Dutch IPF patients 349 healthy controls (HCs). mRNA peripheral...
In animal models, joint degeneration observed in response to obesogenic diet varies nature and severity. this study, we compare damage Sprague Dawley Wistar-Han rats a high-fat, high-sucrose (HFS) groove model of osteoarthritis (OA). Wistar Han (n = 5) were fed an HFS for 24 weeks. OA was induced 12 weeks after the onset by surgery right knee joint. The left served as control. Outcomes OARSI histopathology scoring, bone changes µCT imaging, local (synovial fat pad) systemic (blood cytokine)...
Osteoarthritis (OA) is a degenerative disease of the joints for which no curative treatment exists. Intra-articular injection stem cells explored as regenerative approach, but rapid clearance from site limits therapeutic outcome. Microencapsulation mesenchymal (MSCs) can extend retention time MSCs, outcomes few studies currently performed are conflicting. We hypothesize that composition micromaterial's shell plays deciding factor in outcome intra-articular MSC injection. To this end, we...
Abstract Objective To characterize aspects of triiodothyronine (T3) induced chondrocyte terminal maturation within the molecular osteoarthritis pathophysiology using previously established T3 human ex vivo osteochondral explant model. Designs RNA-sequencing was performed on cartilage obtained from OA patients ( n = 8), that cultured with or without (10 ng/ml), and main findings were validated RT-qPCR in an independent sample set 22). Enrichment analysis used for functional clustering...
Objective To evaluate the presence and localization of folate receptor expressing macrophages in rat groove model osteoarthritis determine suitability a new conjugate with albumin-binding entity (cm09) for vivo SPECT (single-photon emission computed tomography) analysis. Design In male Wistar rats, local cartilage damage was induced addition to standard ( n = 10) or high-fat diet 6). After 12 weeks, 111 labeled conjugates were administered, SPECT/CT (computed imaging performed after 24...