Tissue/organ printing aims to recapitulate the intrinsic complexity of native tissues. For a number tissues, in particular those musculoskeletal origin, adequate mechanical characteristics are an important prerequisite for their initial handling and stability, as well long-lasting functioning. Hence, organized implants, possessing similar tissue, may result improved clinical outcomes regenerative approaches. Using bioprinter, grafts were constructed by alternate deposition thermoplastic...

Osteochondral defects are prone to induce osteoarthritic degenerative changes. Many tissue-engineering approaches that aim generate osteochondral implants suffer from poor tissue formation and compromised integration. This illustrates the need for further improvement of heterogeneous constructs. Engineering these structures is expected profit strategies addressing complexity organization simultaneous use multiple cell types. Moreover, this enables investigation effects three-dimensional (3D)...

Bioprinting is a recent technology in tissue engineering used for the design of porous constructs through layer-by-layer deposition cell-laden material. This would benefit from new biomaterials that can fulfill specific requirements fabrication well-defined 3D constructs, such as preservation cell viability and adequate mechanical properties. We evaluated suitability novel semi-interpenetrating network (semi-IPN), based on hyaluronic acid hydroxyethyl-methacrylate-derivatized dextran...

Abstract Tendon injuries are notorious for their slow and functionally inferior healing. Intratendinous application of platelet‐rich plasma (PRP) has been reported to stimulate the repair process tendon injuries, but there is little conclusive evidence its effectiveness. A placebo‐controlled experimental trial was performed test hypothesis that a single intratendinous PRP treatment enhances quality repair, as evidenced by improved biochemical, biomechanical, histological tissue properties....

In a longitudinal study the hocks and stifles of Dutch Warmblood foals were radiographed at age 1 month subsequently intervals 4 weeks. Forty-three until 5 months 19 11 months. The chance for development osteochondrosis was enhanced by using only offspring from diseased sires with radiographically proven OC either intermediate ridge distal tibia or lateral femoral trochlea. radiographic appearances tibia, aspect trochlear talus midregion trochlea classified on 0-4 scale standardised...

The repair process of tendon injuries, which are common in both human and equine athletes, is slow the quality tissue often inferior to original tissue, frequently leads re-injury. relatively poor vascularization tendons considered be one reasons for their limited healing potential. Recently, platelet-rich plasma (PRP), an autologous concentrate platelets, rich growth factors, has been shown enhance injured tendons. This effect ascribed high levels factors PRP, several known involved repair....

Mammalian articular cartilage serves diverse functions, including shock absorption, force transmission and enabling low-friction joint motion. These challenging requirements are met by the tissue's thickness combined with its highly specific extracellular matrix, consisting of a glycosaminoglycan-interspersed collagen fiber network that provides unique combination resilience high compressive shear resistance. It is unknown how this critical tissue deals challenges posed increases in body...

Multi-material 3D printing technologies that resolve features at different lengths down to the microscale open new avenues for regenerative medicine, particularly in engineering of tissue interfaces. Herein, extrusion a bone-biomimetic ceramic ink and melt electrowriting (MEW) spatially organized polymeric microfibres are integrated biofabrication an osteochondral plug, with mechanically reinforced bone-to-cartilage interface. A printable physiological temperature-setting bioceramic, based...

The crosslinking agent genipin is increasingly invoked for the mechanical augmentation of collagen tissues and implants, has previously been demonstrated to arrest damage accumulation in various tissues. This study established an vitro dose-response baseline effects treatment on tendon cells their matrix, with a view vivo application repair partial tears. Regression models based broad range experimental data were used delineate concentrations that are likely achieve functionally effective...

Decellularized tissues have proven to be versatile matrices for the engineering of and organs. These usually consist collagens, matrix-specific proteins, a set largely undefined growth factors signaling molecules. Although several decellularized found their way clinical applications, use in cartilage tissue has only been explored limited extent. We out generate hydrogels from tissue-derived matrices, as are current preferred cell carriers repair. Equine cartilage, meniscus, tendon was...

In this paper, we describe and validate the EquiMoves system, which aims to support equine veterinarians in assessing lameness gait performance horses. The system works by capturing horse motion from up eight synchronized wireless inertial measurement units. It can be used various modes, analyzes both upper-body limb movements. validation against an optical capture is based on a Bland–Altman analysis that illustrates agreement between two systems. sagittal kinematic results (protraction,...

The main criteria for lameness assessment in horses are head movement forelimb and pelvic hindlimb lameness. However, compensatory nod with primary is a well-known phenomenon. This can be easily misinterpreted as sign of ipsilateral Therefore, discriminating asymmetries from directly pain-related prerequisite successful assessment.To investigate the association between head, withers pelvis asymmetry induced lameness.Experimental study.In 10 clinically sound Warmblood riding horses, were...

Summary The histological aspects of second‐intention healing were studied in 5 horses and ponies. Biopsies taken weekly from standardised wounds on the metatarsus femoral biceps muscle one horse pony. Sections stained to enable cell counting detection DNA synthesis, fibrin, smooth actin (SMA), collagen, bacteria. In ponies, number polymorphonuclear leucocytes (PMNs) was high during first 3 weeks subsequently decreased rapidly. horses, initial PMNs lower, but remained persistently elevated...

To determine kinematic movements of the vertebral column horses during normal locomotion.5 Dutch Warmblood without apparent lameness or problems associated with column.Kinematics 8 vertebrae (T6, T10, T13, T17, L1, L3, L5, and S3) both tuber coxae were determined, using bone-fixated markers. Horses recorded while walking on a treadmill at constant speed 1.6 m/s.Flexion-extension was characterized by 2 periods extension flexion 1 stride cycle, whereas lateral bending axial rotation peak...

Biochemical heterogeneity of cartilage within a joint is well known in mature individuals. It has recently been reported that for proteoglycan content and chondrocyte metabolism sheep develops postnatally under the influence loading. No data exist on collagen network general or specific situation horse. The objective this study was to investigate alterations equine articular biochemistry occur from birth up age one year, testing hypothesis molecular composition matrix uniform at biochemical...

Summary Reasons for performing study : Although the saddle is seen as one of biggest causes back pain, and weightbearing an important aetiological factor in ‘kissing spine’ syndrome (KSS), effects a weight on movements horse have never been studied. Objective To determine pressure back, exerted by tack weight, horse. Hypothesis Weight has extending effect horse's and, compensatory mechanism to this extension, alteration pro‐ retraction angles was expected. A similar but smaller expected from...

Dressage involves training of the horse with head and neck placed in a position defined by rider. The best for dressage is currently under debate among riders trainers, but there are few scientific data available to confirm or disprove different views.To evaluate kinematic effects positions (HNPs) elite horses ridden at trot.Seven high-level were subjected kinetic measurements when on treadmill 5 positions.Compared free trot loose reins HNP desired collected competitions increased T6...

Summary Reasons for performing study : Meloxicam is a commonly used nonsteroidal anti‐inflammatory drug in equine practice, but little known about its vivo effects on joint inflammation and cartilage turnover. Objectives To the of meloxicam biomarkers inflammation, matrix metalloproteinase (MMP) activity, joints with experimental synovitis. Methods In 2‐period cross‐over study, synovitis was induced at T = 0 h L or R intercarpal 6 horses by intraarticular injection 0.5 ng lipopolysaccharide...

Intra-articular administration of morphine as a local analgesic and anti-inflammatory drug is widely used in human medicine. In equids, little known about its clinical efficacy.To use an inflammatory orthopaedic pain model to investigate the effects intra-articularly administered new treatment modality horses with acute arthritis.In crossover study design, synovitis was induced left or right talocrural joint by means intra-articular injection 0.5 ng lipopolyssacharide (LPS). The effect 120...