A5018282061- Mesenchymal stem cell research
- Periodontal Regeneration and Treatments
- Tissue Engineering and Regenerative Medicine
- Hematopoietic Stem Cell Transplantation
- Bone Tissue Engineering Materials
- Electrospun Nanofibers in Biomedical Applications
- Dental Implant Techniques and Outcomes
- Biochemical and Molecular Research
- Chronic Obstructive Pulmonary Disease (COPD) Research
- Osteoarthritis Treatment and Mechanisms
- CAR-T cell therapy research
- Colorectal Cancer Treatments and Studies
- 3D Printing in Biomedical Research
- Pancreatic and Hepatic Oncology Research
- Asthma and respiratory diseases
- Immune Cell Function and Interaction
- Cancer Treatment and Pharmacology
- Multiple Myeloma Research and Treatments
- Acute Myeloid Leukemia Research
- Respiratory Support and Mechanisms
- Hematological disorders and diagnostics
- Amino Acid Enzymes and Metabolism
- Adenosine and Purinergic Signaling
- Biosimilars and Bioanalytical Methods
- Organ and Tissue Transplantation Research
University Medical Center Utrecht
2009-2025
Academic Center for Dentistry Amsterdam
2012-2017
Amsterdam UMC Location Vrije Universiteit Amsterdam
2012-2016
University of Amsterdam
2012-2014
Medisch Centrum Alkmaar
2013
National Medical Research Center of Dentistry and Maxillofacial Surgery
2013
Utrecht University
2011-2013
Taiho Pharmaceutical (Japan)
2005-2010
Wilhelmina Children's Hospital
2009
Mater Misericordiae Hospital
2005
Osteochondral defects are prone to induce osteoarthritic degenerative changes. Many tissue-engineering approaches that aim generate osteochondral implants suffer from poor tissue formation and compromised integration. This illustrates the need for further improvement of heterogeneous constructs. Engineering these structures is expected profit strategies addressing complexity organization simultaneous use multiple cell types. Moreover, this enables investigation effects three-dimensional (3D)...
Earlier, we have shown that the increased cartilage production in pellet co-cultures of chondrocytes and bone marrow-derived mesenchymal stem cells (BM-MSCs) is due to a trophic role MSC stimulating chondrocyte proliferation matrix rather than MSCs actively undergoing chondrogenic differentiation. These studies were performed culture medium was not compatible with differentiation MSCs. In this study, tested whether dependent on culturing co-culture pellets addition, investigated their...
One of the applications bone marrow stromal cells (BMSCs) that are produced by ex vivo expansion is for use in tissue engineering. Cultured a mixture at different stages commitment and capability, leading to heterogeneous cell population each time can differ potential form bone. A parameter predicts forming capacity thus far lacking. We employed single colony-derived BMSC cultures identify such predictive parameters. Using limiting dilution, we have sixteen CFU-F derived from human found...
Adult stem cells, or mesenchymal stromal cells (MSCs), are of great potential for cell therapy and tissue-engineering applications. However, therapeutic use, these need to be isolated from tissue a biopsy efficiently expanded, as they cannot harvested in sufficient quantities the body. In our opinion, efficient expansion MSCs can achieved microcarrier-based cultivation system. This study selected suitable microcarrier human bone marrow-derived (HBMSCs), optimized cell-seeding strategies by...
Abstract In patients undergoing maxillary sinus floor elevation (MSFE) for dental implant placement, bone substitutes are currently evaluated as alternatives autologous bone. However, have only osteoconductive properties and lack osteoinductive potential. Therefore, this phase I study the potential additive effect on regeneration by addition of freshly isolated, but heterologous stromal vascular fraction (SVF), which is highly enriched with adipose stromal/stem cells when compared native...
The role of antibiotics in acute exacerbations chronic obstructive pulmonary disease (COPD) is controversial and a biomarker identifying patients who benefit from mandatory. We performed randomised, controlled trial with COPD, comparing C-reactive protein (CRP)-guided antibiotic treatment to patient reported symptoms accordance the Global Initiative for Chronic Obstructive Lung Disease (GOLD) strategy, order show reduction prescription. Patients hospitalised COPD were randomised receive...
In tissue engineering, strategies are being developed to repair large bone defects by combining biomaterials and marrow-derived multipotent mesenchymal stromal cells (MSCs). For expansion of MSCs under good manufacturing practice conditions, human platelet lysate (PL) can serve as substitute for fetal bovine serum (FBS) in culture media. We compared the vivo bone-forming capacity passage 3 cultured with either PL or FBS nine different donors. also tested growth kinetics, antigen expression...
Adipose-derived stromal cells (ASC) are promising candidates for cell therapy, example to treat myocardial infarction. Commonly, fetal bovine serum (FBS) is used in ASC culturing. However, FBS has several disadvantages. Its effects differ between batches and, when applied clinically, transmission of pathogens and antibody development against possible. In this study, we investigated whether can be substituted by human platelet lysate (PL) culture, without affecting functional capacities...
Mesenchymal stem cells (MSCs) of human and nonhuman mammalian species are often studied for various applications in regenerative medicine research. These MSCs can be derived from bone marrow (BM) identified by their ability to form fibroblast-like colony forming units that develop into stromal like when expanded culture. characterized spindle-shaped morphology, characteristic phenotype (CD73+, CD90+, CD105+, CD45−, CD34−), differentiate the osteogenic, adipogenic, chondrogenic lineages....
The aim of this study was to comparatively evaluate the angiogenic capacity cocultures using either human bone marrow- or adipose tissue-derived mesenchymal stem cells (MSCs) (BM- AT-MSCs) with umbilical vein endothelial (HUVECs) both in vitro and vivo at early time points (i.e. days 3 7). In vitro, were monocultured BM-MSCs, AT-MSCs HUVECs) cocultured BM-MSCs/HUVECs AT-MSCs/HUVECs) on Thermanox® (2-dimensional, 2D) collagen gels (3-dimensional, 3D). For experiment, (cocultures) embedded...
Tissue engineering of bone, by combining multipotent stromal cells (MSCs) with osteoconductive scaffolds, has not yet yielded any clinically useful applications so far. The fate and contribution the seeded are sufficiently clarified, especially at relevant locations. Therefore, we investigated cell proliferation around spine ectopic sites using noninvasive in vivo bioluminescence imaging (BLI) relation to new bone formation. Goat MSCs were lentivirally transduced express luciferase. After...
Hematopoietic stem cell transplantation (HSCT) using umbilical cord blood (UCB) progenitors is increasingly being used. One of the problems that may arise after UCB an impaired engraftment. Either intrabone (IB) injection hematopoietic or mesenchymal (MSC) coadministration has been proposed among strategies to improve In current study, we have assessed effects both approaches. Thus, NOD/SCID recipients were transplanted with human CD34 + cells administered either intravenously (IV) IB,...
Cell-based immunotherapy using donor-derived natural killer (NK) cells after allogeneic hematopoietic stem cell transplantation may be an attractive treatment of residual leukemia. This study aimed to optimize clinical grade production a cytokine-activated NK-cell product. NK were isolated either by double depletion (CD3−, CD19−) or sequential and enrichment CD56+) via CliniMACS from leukapheresis material cultured in vitro with interleukin (IL)-2 IL-15. Both isolation procedures yielded...
Bone marrow (BM) stromal cells (MSCs), also known as mesenchymal stem cells, display a high degree of heterogeneity. To shed light on the causes this heterogeneity, MSCs were collected from either human BM (n=5) or adipose tissue (AT) (n=5), and expanded using 2 different culture methods: one based fetal calf serum, platelet lysate. After initial expansion, frozen, vials transported to 3 laboratories grown for 1 passage same brand plastic, medium, supplements. Subsequently, harvested assayed...
Partial replacement of chondrocytes by stem cells has been proposed to improve the performance autologous chondrocyte implantation (ACI). Our previous studies showed that increased cartilage production in pellet cocultures and mesenchymal (MSCs) is due a trophic role MSCs stimulating proliferation matrix rather than actively undergoing chondrogenic differentiation. The aim this study compare effects stromal vascular fraction (SVF) vitro expanded adipose (ASC). SVF culture-expanded ASC (n =...
The aim of this study was to compare the osteogenic capacity between human adipose tissue-derived mesenchymal stem cells (AT-MSCs) and their cocultures with umbilical vein endothelial (HUVECs) in vitro biological performance vivo. First, optimal cell ratio for differentiation determined by seeding AT-MSCs HUVECs ratios varying from 100:0 0:100 on tissue culture plates. Afterward, AT-MSCs/HUVECs (50:50) were seeded porous titanium fiber mesh scaffolds (Ti) both vivo evaluation. For...
Human bone marrow-derived mesenchymal stem cells (BM-MSCs) and human adipose tissue-derived (AT-MSCs) are the most frequently used in tissue engineering. Due to major clinical demands, it is necessary find an optimally safe efficient way for large-scale expansion of these cells. Considering nutritional source culture medium method, this study aimed analyze effects FBS- PL-supplemented media on osteogenesis cell mono- co-cultures with umbilical vein endothelial (HUVECs). Results showed that...
Abstract Trifluorothymidine (TFT) is part of the novel oral formulation TAS-102, which currently evaluated in phase II studies. Drug resistance an important limitation cancer therapy. The aim present study was to induce TFT H630 colon cells using two different schedules and analyze mechanism. Cells were exposed either continuously or intermittently TFT, resulting H630-cTFT H630-4TFT, respectively. analyzed for cross-resistance, cell cycle, protein expression, activity thymidine phosphorylase...
Calcium phosphates are used in maxillary sinus floor elevation (MSFE) procedures to increase bone height prior dental implant placement. Whether a collagenous barrier membrane coverage of the lateral window affects formation within substitute augmentation is currently an important matter debate, since its benefit has not been irrefutably proven. Therefore, this clinical study twelve patients underwent MSFE procedure with β-tricalcium phosphate (β-TCP). The was either left uncovered, or...
The Hollow Fibre Assay (HFA) is usually applied as an early in vivo model for anti-cancer drug screening, but potentially excellent short-term pharmacodynamic studies. We used the to study role of thymidine phosphorylase/platelet-derived endothelial cell growth factor (TP/PD-ECGF) cytotoxicity and pharmacodynamics TAS-102 colon cancer cells. a new oral formulation, which composed trifluorothymidine (TFT) phosphorylase inhibitor (TPI), prevents TFT degradation. compared activity with Xeloda...