A5001166940- Mesenchymal stem cell research
- Hematopoietic Stem Cell Transplantation
- Tissue Engineering and Regenerative Medicine
- Cancer Cells and Metastasis
- Neonatal Respiratory Health Research
- Cytomegalovirus and herpesvirus research
- Bone and Joint Diseases
- Sarcoma Diagnosis and Treatment
- Inflammatory Bowel Disease
- Testicular diseases and treatments
- Organ and Tissue Transplantation Research
- Immunotherapy and Immune Responses
- Anorectal Disease Treatments and Outcomes
- Immune cells in cancer
- Wound Healing and Treatments
- Telomeres, Telomerase, and Senescence
- Extracellular vesicles in disease
- MicroRNA in disease regulation
- Cardiac Fibrosis and Remodeling
- T-cell and B-cell Immunology
- Diverticular Disease and Complications
- Cancer Genomics and Diagnostics
- Stoma care and complications
- Immunodeficiency and Autoimmune Disorders
- Bacteriophages and microbial interactions
Leiden University Medical Center
2011-2021
Leiden University
1992-2019
Amsterdam UMC Location University of Amsterdam
2002-2006
University Medical Center Utrecht
2006
University of Amsterdam
2002
Sanquin
2002
Rotterdam University of Applied Sciences
1998-1999
<h3>Background and aim</h3> Mesenchymal stromal cells (MSCs) are pluripotent that have immunosuppressive effects both in vitro experimental colitis. Promising results of MSC therapy been obtained patients with severe graft versus host disease the gut. Our objective was to determine safety feasibility autologous bone marrow derived refractory Crohn9s disease. <h3>Patients intervention</h3> 10 adult (eight females two males) underwent aspiration under local anaesthesia. Bone MSCs were isolated...
Abstract Adipose tissue-derived multipotent stromal cells (AT-MSCs) are studied as an alternative to bone marrow-derived (BM-MSCs) for immunomodulatory treatment. In this study, we systematically compared the capacities of BM-MSCs and AT-MSCs derived from age-matched donors. We found that share a similar immunophenotype capacity in vitro multilineage differentiation. showed comparable effects they were both able suppress proliferation stimulated peripheral blood mononuclear inhibit...
Abstract Multipotent stromal cells (MSC) have been shown to possess immunomodulatory capacities and are therefore explored as a novel cellular therapy. One of the mechanisms through which MSC modulate immune responses is by promotion regulatory T cell (Treg) formation. In this study, we focused on interactions secreted factors that essential in process. Using an vitro culture system, showed culture-expanded bone marrow-derived promote generation CD4+CD25hiFoxP3+ human PBMC populations these...
Background Conventionally, mesenchymal stem cells are functionally isolated from primary tissue based on their capacity to adhere a plastic surface. This isolation procedure is hampered by the unpredictable influence of co-cultured hematopoietic and/or other unrelated elimination late adhering subset during removal undesired cells. To circumvent these limitations, several antibodies have been developed facilitate prospective Recently, we described panel monoclonal with superior selectivity...
Mesenchymal stromal cells (MSCs) are currently under investigation for the treatment of inflammatory disorders, including Crohn's disease. MSCs pluripotent with immunosuppressive properties. Recent data suggest that resting do not have significant immunomodulatory activity, but function has to be elicited by interferon-γ (IFN-γ). In this article, we assessed effects IFN-γ prestimulation (IMSCs) on their properties in vitro and vivo. To end, pretreated therapeutic dextran sodium sulfate...
Abstract Despite excellent short-term results, long-term survival of transplanted kidneys has not improved accordingly. Although alloimmune responses and calcineurin inhibitor-related nephrotoxicity have been identified as main drivers fibrosis, no effective treatment options emerged. In this perspective, mesenchymal stromal cells (MSCs) are an interesting candidate because their immunosuppressive regenerative properties. Of importance, other clinical studies investigated effects in...
Summary Mesenchymal stromal cell ( MSC ) infusions have been reported to be effective in patients with steroid‐refractory, acute graft‐versus‐host disease (a G v HD but comprehensive data on paediatric are limited. We retrospectively analysed a cohort of 37 children (aged 3 months‐17 years) treated s for steroid‐refractory grade III – IV . All three received multiple infusions. Complete response CR was observed 24 (65%), while 13 had either partial n = 8) or no 5). Cumulative incidence...
Multipotent stromal cells have immunomodulatory capacities and been used in transplantation autoimmune diseases. One of the effects multipotent involves inhibition dendritic cell differentiation. Since interleukin-6 interleukin-10 are known to play a role inhibiting immature differentiation, we hypothesized that these cytokines may also mediate inhibitory effect human In order test this hypothesis monocytes were cultured with interleukin-4 granulocyte-monocyte colony-stimulating factor...
Stem cells are widely studied to enable their use in tissue repair. However, differences function and differentiation potential exist between distinct stem cell populations. Whether those due donor variation, culture, or intrinsic properties remains elusive. Therefore, we compared 3 lines isolated from different niches using the Affymetrix Exon Array platform: cord blood-derived neonatal unrestricted somatic (USSC), adult bone marrow-derived mesenchymal (BM-MSC), adipose tissue-derived...
Abstract High‐grade osteosarcoma is a tumor with complex genomic profile, occurring primarily in adolescents second peak at middle age. The extensive alterations obscure the identification of genes driving tumorigenesis during development. To identify such driver genes, we integrated DNA copy number profiles (Affymetrix SNP 6.0) 32 diagnostic biopsies 84 expression (Illumina Human‐6 v2.0) high‐grade as compared its putative progenitor cells, i.e., mesenchymal stem cells ( n = 12) or...
The long-term safety and efficacy of allogeneic bone marrow-derived mesenchymal stromal cell [bmMSC] therapy in perianal Crohn's disease [CD] fistulas is unknown. We aimed to provide a 4-year clinical evaluation bmMSC treatment CD fistulas.A double-blind dose-finding study for local 21 patients with refractory fistulising was performed at the Leiden University Medical Center 2012-2014. All treated bmMSCs [1 x 107 cohort 1, n = 5; 3 × 2, 9 3, 5] were invited evaluation. Clinical events...
Background: Mesenchymal stromal cells (MSCs) may reduce inflammation and promote tissue repair in pulmonary emphysema. Aim: To study the safety feasibility of bone marrow-derived autologous (BM-) MSC intravenous administration to patients with severe Design: A phase I, prospective open-label registered at ClinicalTrials.gov as NCT01306513. Eligible had lung volume reduction surgery (LVRS) on two separate occasions. During first LVRS marrow was collected, from which MSCs were isolated...
Mesenchymal stem cells (MSCs) are multipotent capable of differentiation into several mesodermal lineages. These have been isolated from various tissues, such as adult bone marrow, placenta, and fetal tissues. The comparative potential these originating different tissues to differentiate the chondrogenic lineage is still not fully defined. aim our study was investigate MSCs sources. were phenotypically characterized examined for their capacity, based on morphological criteria expression...
The low percentages of naïve T cells commonly observed in elderly people are thought to be causally associated with mortality, primarily from infectious disease, and taken as a hallmark "immunosenescence". Whether levels naive actually do associate mortality has, however, not been tested longitudinal studies. Here, we present correlations between peripheral T-cell phenotypes 8-year survival individuals the population-based prospective Leiden 85-plus Study. Counter-intuitively, found that...
In experimental studies, mesenchymal stem cell (MSC) transplantation in acute myocardial infarction (AMI) models has been associated with enhanced neovascularization and myogenesis. Clinical data however, are scarce. Therefore, the present study evaluates safety feasibility of intramyocardial MSC injection nine patients, shortly after AMI during short-term 5-year follow-up. Periprocedural analysis demonstrated one transient ischemic attack. No other adverse events related to treatment were...
Mesenchymal stromal cells (MSCs) are investigated for their potential to reduce inflammation and repair damaged tissue. Inflammation tissue damage hallmarks of chronic obstructive pulmonary disease (COPD) MSC infusion is a promising new treatment COPD. Inflammatory mediators attract MSCs sites affect immune-modulatory properties, but little known about effect on regenerative properties MSCs. This study investigates the pro-inflammatory cytokines TNF-α IL-1β MSCs, using an in vitro wound...
The immunosuppressive properties of mesenchymal stromal cells (MSC) have been successfully tested to control clinical severe graft-versus host disease and improve survival. However, studies not yet provided conclusive evidence their efficacy largely because lack patients' stratification criteria. heterogeneity MSC preparations is also a major contributing factor, as manufacturing therapeutic performed according different protocols among centers. Understanding the variability protocol would...
Investigation into predictors for treatment outcome is essential to improve the clinical efficacy of therapeutic multipotent mesenchymal stromal cells (MSCs). We therefore studied possible harmful impact immunogenic ABO blood groups antigens - genetically governed antigenic determinants at all given steps MSC-therapy, from cell isolation and preparation use, final recipient outcome. found that MSCs do not inherently express or upregulate group after inflammatory challenge in vitro...