A5045837453- Mesenchymal stem cell research
- Tissue Engineering and Regenerative Medicine
- Cardiac Fibrosis and Remodeling
- Cardiovascular Function and Risk Factors
- Cardiac electrophysiology and arrhythmias
- Cardiac Imaging and Diagnostics
- Acute Myocardial Infarction Research
- Cardiomyopathy and Myosin Studies
- Cardiovascular Effects of Exercise
- Coronary Interventions and Diagnostics
- Congenital heart defects research
- Electrospun Nanofibers in Biomedical Applications
- Telemedicine and Telehealth Implementation
- Mobile Health and mHealth Applications
- Cardiac pacing and defibrillation studies
- Cardiac Health and Mental Health
- Digital Mental Health Interventions
- Cardiac Ischemia and Reperfusion
- Pluripotent Stem Cells Research
- Cardiac Structural Anomalies and Repair
- Cardiac Valve Diseases and Treatments
- Mechanical Circulatory Support Devices
- Heart Failure Treatment and Management
- Signaling Pathways in Disease
- Neuroscience and Neural Engineering
Leiden University Medical Center
2016-2025
Leiden University
2009-2025
Delft University of Technology
2023-2025
Pharmo Institute
2023
Erasmus University Rotterdam
2009-2023
Creative Commons
2023
Cleveland Clinic
2017
AZ Sint-Jan
2017
Loyola University Medical Center
2008-2014
Medical University of Vienna
2013
A more accurate means of prognostication in breast cancer will improve the selection patients for adjuvant systemic therapy.
Background— Arrhythmogenic right ventricular dysplasia/cardiomyopathy (ARVD/C) is a progressive cardiomyopathy. We aimed to define long-term outcome in transatlantic cohort of 1001 individuals. Methods and Results— Clinical genetic characteristics follow-up data ARVD/C index-patients (n=439, fulfilling 2010 criteria all) family members (n=562) were assessed. Mutations identified 276 (63%). Index-patients presented predominantly with sustained arrhythmias (268; 61%). During median 7 years,...
We sought to determine the influence of genotype on clinical course and arrhythmic outcome among arrhythmogenic right ventricular dysplasia/cardiomyopathy (ARVD/C)-associated mutation carriers.Pathogenic mutations in desmosomal non-desmosomal genes were identified 577 patients (241 families) from USA Dutch ARVD/C cohorts. Patients with sudden cardiac death (SCD)/ventricular fibrillation (VF) at presentation (n = 36) younger (median 23 vs. 36 years; P < 0.001) than those presenting sustained...
Previous studies have suggested that bone marrow cell injection may improve myocardial perfusion and left ventricular (LV) function in patients with chronic ischemia.To investigate the effect of intramyocardial on LV ischemia.Randomized, double-blind, placebo-controlled trial at a Netherlands university hospital, May 1, 2005-March 3, 2008 (6-month follow-up ended September 2008) 50 ischemia (mean age [SD], 64 [8] years; 43 men).severe angina pectoris despite optimal medical therapy ischemia....
Maximizing baseline function of human pluripotent stem cell-derived cardiomyocytes (hPSC-CMs) is essential for their effective application in models cardiac toxicity and disease. Here, we aimed to identify factors that would promote an adequate level permit robust single-cell contractility measurements a induced cell (hiPSC) model hypertrophic cardiomyopathy (HCM). A simple screen revealed the collaborative effects thyroid hormone, IGF-1 glucocorticoid analog dexamethasone on...
Arrhythmogenic right ventricular dysplasia/cardiomyopathy (ARVD/C) is an autosomal dominant inherited disease with incomplete penetrance and variable expression. Causative mutations in genes encoding 5 desmosomal proteins are found ≈50% of ARVD/C index patients. Previous genotype-phenotype relation studies involved mainly overt patients, so follow-up data on relatives scarce.One hundred forty-nine patients (111 male patients; age, 49±13 years) according to 2010 Task Force criteria 302 from...
Abstract Previous studies have demonstrated that cGMP and cAMP reduce the endothelial permeability for fluids macromolecules when is increased by thrombin. In this study, we investigated mechanism which improves barrier function examined whether nitric oxide (NO) can serve as an endogenous modulator of function. Thrombin passage through human umbilical vein aortic cell monolayers concomitantly [Ca] 2+ in vitro. Inhibition these increases intracellular Ca chelator BAPTA indicated cytoplasmic...
Elevated cardiac troponin-I (cTnI) levels have been demonstrated in serum of patients without acute coronary syndromes, potentially via a stretch-related process. We hypothesize that this cTnI release from viable cardiomyocytes is mediated by stimulation stretch-responsive integrins. Cultured were treated with (1) Gly-Arg-Gly-Asp-Ser (GRGDS, n = 22) to stimulate integrins, (2) Ser-Asp-Gly-Arg-Gly (SDGRG, 8) does not or (3) phosphate-buffered saline (control, 38). Cells and media analyzed for...
Significance There are few laboratory models that recapitulate human cardiac disease. Here, we created cell for Jervell and Lange-Nielsen syndrome (JLNS) in vitro, based on induced pluripotent stem cells (hiPSCs). JLNS is one of the most severe disorders heart rhythm can cause sudden death young patients. inherited recessively caused by homozygous mutations slow component delayed rectifier potassium current, I Ks . Cardiomyocytes (CMs) from two independent sets patient-derived engineered...
Abstract Myocardial and coronary development are both critically dependent on epicardial cells. During cardiomorphogenesis, a subset of cells undergoes an epithelial-to-mesenchymal transition (EMT) invades the myocardium to differentiate into various cell types, including smooth muscle perivascular cardiac interstitial fibroblasts. Our current knowledge EMT ensuing epicardium-derived (EPDCs) comes primarily from studies chick mouse embryonic development. Due absence in vitro culture system,...
The value of left atrial (LA) diameter, volume, and strain to risk stratify hypertrophic cardiomyopathy patients for new-onset fibrillation (AF) was explored.A total 242 without AF history were evaluated by (speckle-tracking) echocardiography. During mean follow-up 4.8±3.7 years, 41 (17%) developed AF. Multivariable analysis showed LA volume (≥37 mL/m2; hazard ratio, 2.68; 95% confidence interval, 1.30-5.54; P=0.008) (≤23.4%; 3.22; 1.50-6.88; P=0.003), but not diameter (≥45 mm; 1.67;...
Alignment of cardiomyocytes (CMCs) contributes to the anisotropic (direction-related) tissue structure heart, thereby facilitating efficient electrical and mechanical activation ventricles. This study aimed investigate effects forced alignment stem cells during cardiomyogenic differentiation on their functional integration with CMC cultures. Labeled neonatal rat (nr) mesenchymal (nrMSCs) were allowed differentiate into heart muscle in different cell-alignment patterns 10 days coculture...
Despite significant efforts, the COVID-19 pandemic has put enormous pressure on health care systems around world, threatening quality of patient care. Telemonitoring offers opportunity to carefully monitor patients with a confirmed or suspected case from home and allows for timely identification worsening symptoms. Additionally, it may decrease number hospital visits admissions, thereby reducing use scarce resources, optimizing capacity, minimizing risk viral transmission. In this paper, we...
<h3>Importance</h3> Smart technology via smartphone-compatible devices might improve blood pressure (BP) regulation in patients after myocardial infarction. <h3>Objectives</h3> To investigate whether smart clinical practice can BP and to evaluate the feasibility of such an intervention. <h3>Design, Setting, Participants</h3> This study was investigator-initiated, single-center, nonblinded, feasibility, randomized trial conducted at Department Cardiology Leiden University Medical Center...
Abstract Aims Little is known about the impact of daily physical activity timing (here referred to as ‘chronoactivity’) on cardiovascular disease (CVD) risk. We aimed examined associations between chronoactivity and multiple CVD outcomes in UK Biobank. Methods results data were collected UK-Biobank through triaxial accelerometer over a 7-day measurement period. used K-means clustering create clusters participants with similar irrespective mean intensity activity. Multivariable-adjusted...
In 21 patients, the authors compared results with quantitative gated single photon emission computed tomography (SPECT) to magnetic resonance imaging in assessment of left ventricular (LV) end-diastolic volume (LVEDV), end-systolic (LVESV), and ejection fraction (LVEF). Between two methods, correlations were good for LVEF (r = 0.85), LVEDV 0.94), LVESV 0.95). Quantitative SPECT can help determine LVEF, LVEDV, LVESV.
Proper development of compact myocardium, coronary vessels, and Purkinje fibers depends on the presence epicardium-derived cells (EPDCs) in embryonic myocardium. We hypothesized that adult human EPDCs might partly reactivate their program when transplanted into ischemic myocardium improve cardiac performance after myocardial infarction.EPDCs were isolated from atrial tissue. Myocardial infarction was created immunodeficient mice, followed by intramyocardial injection 4x10(5) enhanced green...