A5072311778- Congenital heart defects research
- Congenital Heart Disease Studies
- Cardiac Valve Diseases and Treatments
- Pulmonary Hypertension Research and Treatments
- Metabolism and Genetic Disorders
- Colorectal Cancer Surgical Treatments
- Aortic Disease and Treatment Approaches
- Coronary Artery Anomalies
- Pelvic floor disorders treatments
- Cardiac Arrhythmias and Treatments
- Cardiovascular Conditions and Treatments
- Anorectal Disease Treatments and Outcomes
- Hernia repair and management
- Tissue Engineering and Regenerative Medicine
- Porphyrin Metabolism and Disorders
- Congenital Diaphragmatic Hernia Studies
- Endometrial and Cervical Cancer Treatments
- Skin and Cellular Biology Research
- Aortic aneurysm repair treatments
- Biochemical and Molecular Research
- Cardiomyopathy and Myosin Studies
- Genetic and Kidney Cyst Diseases
- Genomic variations and chromosomal abnormalities
- Dermatological and Skeletal Disorders
- Cutaneous lymphoproliferative disorders research
Leiden University Medical Center
2016-2025
Leiden University
2007-2025
Università Cattolica del Sacro Cuore
2018
Cancer Genomics Centre
2015
Catharina Ziekenhuis
2008
Radboud University Nijmegen
2008
Academic Medical Center
2006-2008
University Medical Center Utrecht
2007
University of Amsterdam
2006
Erasmus MC
2004-2005
All blood vessels are lined by endothelium and, except for the capillaries, surrounded one or more layers of smooth muscle cells. The origin embryonic vascular cell has until now been described from neural crest and locally differentiating mesenchyme. In this study, we have substantial evidence that quail endothelial cells competent in dorsal aorta embryo to transdifferentiate into subendothelial mesenchymal expressing actins vivo. At onset differentiation, QH1-positive were experimentally...
Autosomal dominant polycystic kidney disease (ADPKD) is a major cause of renal failure and characterized by the formation many fluid-filled cysts in kidneys. It systemic disorder that caused mutations PKD1 or PKD2. Homozygous inactivation these genes at cellular level, 'two-hit' mechanism, has been implicated cyst but does not seem to be sole mechanism for cystogenesis. We have generated novel mouse model with hypomorphic Pkd1 allele, Pkd1(nl), harbouring an intronic neomycin-selectable...
Abstract —In this study, the distribution patterns of neural crest (NC) cells (NCCs) in developing vascular system chick were thoroughly studied and examined for a correlation with smooth muscle cell differentiation morphogenesis. For purpose, we performed long-term lineage tracing using quail-chick chimera techniques premigratory NCC infection replication-incompetent retrovirus containing LacZ reporter gene combination immunohistochemistry. Results indicate that deposition around...
Objective: Several decellularisation techniques have been developed to produce acellular matrix scaffolds for the purpose of tissue engineering, mostly comprising (non-)ionic detergents or enzymatic extraction methods. However, effect chemically induced on major structural and adhesion molecules as well glycosaminoglycans, possible replenishment lost compounds escaped attention. Methods: Porcine aortic valves were treated with two different methods: detergent Triton X-100 Trypsine cell...
Abstract To study the role of blood flow in normal and abnormal heart development, an embryonic chicken model was developed. The effect altered venous inflow on intracardiac patterns studied by visualization with India ink. At stage 17, ink injected into a capillary or small venule within specific yolk sac region. After determination pattern, right lateral vitelline vein ligated, new pattern studied. Ligation resulted disturbance patterns, which most obvious conotruncus. long-term these...
Surgical damage to the pelvic autonomic nerves during radical hysterectomy is thought be responsible for considerable morbidity, i.e., impaired bladder function, defecation problems, and sexual dysfunction. Previous anatomical studies detailed study of surgical techniques in various Japanese oncology centers demonstrated that anatomy nerve plexus permits a systematic approach preserve these without compromising radicality. We introduced elements nerve-preserving carried out feasibility ten...
Abstract Primary cilia are mechanosensors for fluid shear stress, and involved in a number of syndromes congenital anomalies. We identified endothelial areas low stress the embryonic heart. The objective present study was to demonstrate role primary mechanosensing. Ciliated cells were cultured from heart, non‐ciliated arteries. Non‐ciliated that subjected showed significantly less induction marker Kr ü ppel ‐ Like Factor 2 , as compared ciliated cells. In addition, which chemically removed...
Total mesorectal excision (TME) for rectal cancer may result in anorectal and urogenital dysfunction. We aimed to study possible nerve disruption during TME its consequences functional outcome. Because the levator ani muscle plays an important role both urinary fecal continence, explanation could be peroperative damage of supply muscle.TME was performed on cadaver pelves. Subsequently, anatomy pelvic floor innervation relation autonomic mesorectum were studied. Additionally, data from Dutch...
Abstract Epicardium and epicardium‐derived cells have been shown to be necessary for myocardial differentiation. To elucidate the function of podoplanin in epicardial development differentiation, we analyzed knockout mouse embryos between embryonic day (E) 9.5 E15.5 using immunohistochemical differentiation markers, morphometry, three‐dimensional reconstructions. Podoplanin null mice an increased lethality, possibly cardiac origin. Our study reveals impairment proepicardial organ, adhesion,...
Abnormal atrial automaticity in young patients with structurally normal hearts is often located around the pulmonary veins and sinus venosus-related parts of right atrium. We hypothesize that these ectopic pacemaker sites correspond to areas embryonic myocardium an early phenotypic differentiation, as indicated by differences antigen expression during cardiac development.In human embryos ranging age from 42 54 days gestation, development conduction system was studied use HNK-1...
Proper development of compact myocardium, coronary vessels, and Purkinje fibers depends on the presence epicardium-derived cells (EPDCs) in embryonic myocardium. We hypothesized that adult human EPDCs might partly reactivate their program when transplanted into ischemic myocardium improve cardiac performance after myocardial infarction.EPDCs were isolated from atrial tissue. Myocardial infarction was created immunodeficient mice, followed by intramyocardial injection 4x10(5) enhanced green...
<h3>Objective</h3> In ovarian cancer, detection of sentinel nodes is an upcoming procedure. Perioperative determination the patient’s node(s) might prevent a radical lymphadenectomy and associated morbidity. It essential to understand lymphatic drainage pathways ovaries, which are surprisingly up till now poorly investigated, predict anatomical regions where can be found. We aimed describe human ovaries including their compartmental fascia borders. <h3>Methods</h3> A series 3 female fetuses...
Primary cilia are cellular protrusions involved in mechanic and chemical sensing on almost all cells of our body. Important signaling pathways, including Hedgehog, TGFβ, Ca(2+), linked to and/or function. Cilia can vary length, which has functional implications. To measure these lengths correctly, a standardized method with high reliability throughput is required. date, methods for length measurements cultured after fluorescent staining ciliary components error prone possible human selection...