A5001894450- Pluripotent Stem Cells Research
- 3D Printing in Biomedical Research
- Tissue Engineering and Regenerative Medicine
- Congenital heart defects research
- CRISPR and Genetic Engineering
- Neuroscience and Neural Engineering
- Cardiomyopathy and Myosin Studies
- Cardiac electrophysiology and arrhythmias
- Electrospun Nanofibers in Biomedical Applications
- Medical Imaging Techniques and Applications
- RNA Research and Splicing
- Innovative Microfluidic and Catalytic Techniques Innovation
- Medical and Biological Sciences
- Immunotherapy and Immune Responses
- Cell Adhesion Molecules Research
- RNA modifications and cancer
- Microfluidic and Capillary Electrophoresis Applications
- Microfluidic and Bio-sensing Technologies
- Genomics and Chromatin Dynamics
- RNA and protein synthesis mechanisms
- Renal and related cancers
- Mesenchymal stem cell research
- Cellular Mechanics and Interactions
- Virus-based gene therapy research
- Ion channel regulation and function
University of Twente
2016-2025
Leiden University
2013-2025
Leiden University Medical Center
2011-2023
Stem Cell Technology (Taiwan)
2016-2020
Royal Netherlands Academy of Arts and Sciences
2017
University of Glasgow
2017
Amsterdam UMC Location University of Amsterdam
2011
Newcastle University
2011
Hubrecht Institute for Developmental Biology and Stem Cell Research
2002-2010
Utrecht University
2007-2010
Background— Cardiomyocytes derived from human embryonic stem (hES) cells could be useful in restoring heart function after myocardial infarction or failure. Here, we induced cardiomyocyte differentiation of hES by a novel method and compared their electrophysiological properties coupling with those primary fetal cardiomyocytes. Methods Results— were cocultured visceral-endoderm (VE)–like the mouse. This initiated to beating muscle. Sarcomeric marker proteins, chronotropic responses, ion...
Although it is generally accepted that cellular differentiation requires changes to transcriptional networks, dynamic regulation of promoters and enhancers at specific sets genes has not been previously studied en masse. Exploiting the fact active are transcribed, we simultaneously measured their activity in 19 human 14 mouse time courses covering a wide range cell types biological stimuli. Enhancer RNAs, then messenger RNAs encoding transcription factors, dominated earliest responses....
Defined growth conditions are essential for many applications of human embryonic stem cells (hESC). Most defined media presently used in combination with Matrigel, a partially extracellular matrix (ECM) extract from mouse sarcoma. Here, we ECM requirements hESC by analyzing integrin expression and production determined function using blocking antibodies. expressed all major proteins corresponding integrins. We then systematically replaced Matrigel medium supplements proteins. Cells attached...
Studying blood clotting in stereolithography 3D-printed microfluidic chips with endothelialised vascular structures.
Maximizing baseline function of human pluripotent stem cell-derived cardiomyocytes (hPSC-CMs) is essential for their effective application in models cardiac toxicity and disease. Here, we aimed to identify factors that would promote an adequate level permit robust single-cell contractility measurements a induced cell (hiPSC) model hypertrophic cardiomyopathy (HCM). A simple screen revealed the collaborative effects thyroid hormone, IGF-1 glucocorticoid analog dexamethasone on...
Human embryonic stem cells (hESCs) can differentiate into cardiomyocytes, but the efficiency of this process is low. We routinely induce cardiomyocyte differentiation HES‐2 cell line by coculture with a visceral endoderm‐like line, END‐2, in presence 20% fetal calf serum (FCS). In study, we demonstrate striking inverse relationship between and concentration FCS during HES‐2‐END‐2 coculture. The number beating areas cocultures was increased 24‐fold absence compared FCS. An additional 40%...
Calmodulin kinase (CaMK) II is linked to arrhythmia mechanisms in cellular models where repolarization prolonged. CaMKII upregulation and prolonged are general features of cardiomyopathy, but the role arrhythmias cardiomyopathy unknown.We studied a mouse model cardiac hypertrophy attributable transgenic (TG) overexpression constitutively active form CaMKIV that also has increased endogenous activity. ECG-telemetered TG mice had significantly more than wild-type (WT) littermate controls at...
Cell transplantation may restore heart function in disease associated with loss or dysfunction of cardiomyocytes. Recently, Laflamme et al reported an improvement cardiac immunodeficient rats 4 weeks after coronary artery ligation and injection human embryonic stem cell-derived cardiomyocytes (hESC-CMs). We have recently carried out a comparable study transplanting hESC-CMs to the hearts mice myocardial infarction. Our findings were similar up 4-week time point, significant improvements...
Mammals are unable to regenerate their heart after major cardiomyocyte loss caused by myocardial infarction. Human embryonic stem cells (hESCs) can give rise functional cardiomyocytes and therefore have exciting potential as a source of for replacement therapy. Understanding the molecular regulation differentiation from is crucial stepwise enhancement scaling production that will be necessary transplantation Our novel hESC protocol now efficient enough meaningful genome-wide transcriptional...
Differentiated derivatives of human pluripotent stem cells (hPSCs) are often considered immature because they resemble foetal more than adult, with hPSC-derived cardiomyocytes (hPSC-CMs) being no exception. Many functional features these CMs, such as their cell morphology, electrophysiological characteristics, sarcomere organization and contraction force, underdeveloped compared to adult cardiomyocytes. However relatively little is known on how gene expression profiles compare the heart, in...