A5062434400- Ultrasound and Hyperthermia Applications
- Ultrasound and Cavitation Phenomena
- Uterine Myomas and Treatments
- Endometriosis Research and Treatment
- Tissue Engineering and Regenerative Medicine
- Mesenchymal stem cell research
- Electrospun Nanofibers in Biomedical Applications
- Photoacoustic and Ultrasonic Imaging
- Viral Infections and Immunology Research
- Ultrasound Imaging and Elastography
- Gynecological conditions and treatments
- Endometrial and Cervical Cancer Treatments
- Maternal and fetal healthcare
- Microfluidic and Bio-sensing Technologies
- Microencapsulation and Drying Processes
- Biomarkers in Disease Mechanisms
- Sepsis Diagnosis and Treatment
- Cardiovascular Disease and Adiposity
- RNA Interference and Gene Delivery
- Cardiac Imaging and Diagnostics
- Eosinophilic Disorders and Syndromes
- Infective Endocarditis Diagnosis and Management
- Clusterin in disease pathology
- Virus-based gene therapy research
- Pericarditis and Cardiac Tamponade
Amsterdam University Medical Centers
2014-2025
Vrije Universiteit Amsterdam
2011-2025
Amsterdam UMC Location Vrije Universiteit Amsterdam
2010-2021
Netherlands Heart Institute
2007-2021
Amsterdam UMC Location University of Amsterdam
2018
University Medical Center
2014
University Hospital and Clinics
2014
University of Groningen
2009-2013
University Medical Center Groningen
2009-2013
Erasmus MC
2009
Contrast microbubbles in combination with ultrasound (US) are promising vehicles for local drug and gene delivery. However, the exact mechanisms behind intracellular delivery of therapeutic compounds remain to be resolved. We hypothesized that endocytosis pore formation involved during US microbubble targeted (UMTD) compounds. Therefore, primary endothelial cells were subjected UMTD fluorescent dextrans (4.4 500 kDa) using 1 MHz pulsed 0.22-MPa peak-negative pressure, 30 seconds....
In the present study, we addressed interactions among ultrasound, microbubbles, and living cells as well consequent arising bioeffects. We specifically investigated whether hydrogen peroxide (H(2)O(2)) is involved in transient permeabilization of cell membranes vitro after ultrasound exposure at low diagnostic power, presence stable oscillating by measuring generation H(2)O(2) Ca(2+) influx. Ultrasound, absence or SonoVue was applied to H9c2 1.8 MHz with a mechanical index (MI) 0.1 0.5...
Adipose-derived stromal cells (ASC) are promising candidates for cell therapy, example to treat myocardial infarction. Commonly, fetal bovine serum (FBS) is used in ASC culturing. However, FBS has several disadvantages. Its effects differ between batches and, when applied clinically, transmission of pathogens and antibody development against possible. In this study, we investigated whether can be substituted by human platelet lysate (PL) culture, without affecting functional capacities...
Apoptosis of endothelial cells related to homocysteine (Hcy) has been reported in several studies. In this study, we evaluated whether reactive oxygen species (ROS)-producing signaling pathways contribute Hcy-induced apoptosis induction, with specific emphasis on NADPH oxidases. Human umbilical vein were incubated 0.01–2.5 mM Hcy. We determined the effect Hcy caspase-3 activity, annexin V positivity, intracellular NOX1, NOX2, NOX4, and p47phox expression localization, nuclear nitrotyrosine...
Eur J Clin Invest 2010; 40 (10): 893–902 Abstract Background Clusterin (Apolipoprotein J), a plasma protein with cytoprotective and complement‐inhibiting activities, localizes in the infarcted heart during myocardial infarction (MI). Recently, we have shown protective effect of exogenous clusterin vitro on ischaemically challenged cardiomyocytes independent complement. We therefore hypothesized that intravenous administration would reduce damage. Methods Wistar rats undergoing experimental...
The use of stem cells for the repair damaged cardiac tissue after a myocardial infarction holds great promise. However, common finding in experimental studies is low number delivered at area risk. To improve delivery, we are currently investigating novel delivery platform which conjugated with targeted microbubbles, creating echogenic complexes dubbed StemBells. These StemBells vibrate response to incoming ultrasound waves making them susceptible acoustic radiation force. force can then be...
Successful stem cell therapy after acute myocardial infarction (AMI) is hindered by lack of engraftment sufficient cells at the site injury. We designed a novel technique to overcome this problem assembling cell-microbubble complexes, named 'StemBells'. StemBells were assembled through binding dual-targeted microbubbles (~ 3 μm) adipose-derived (ASCs) via CD90 antibody. targeted infarct area an ICAM-1 antibody on microbubbles. characterized microscopically and flow cytometry. The effect...
Although lymphocytic myocarditis (LM) clinically can mimic myocardial infarction (MI), they are regarded as distinct clinical entities. However, we observed a high prevalence (32%) of recent MI in patients diagnosed post-mortem with LM. To investigate if LM changes coronary atherosclerotic plaque, analyzed autopsied hearts the inflammatory infiltrate and stability lesions and/or MI.The three main arteries were isolated at autopsy LM, 3-6h old, old (LM+MI) controls. In tissue sections...