A5056961705- Interstitial Lung Diseases and Idiopathic Pulmonary Fibrosis
- COVID-19 Clinical Research Studies
- SARS-CoV-2 and COVID-19 Research
- Sarcoidosis and Beryllium Toxicity Research
- SARS-CoV-2 detection and testing
- Allergic Rhinitis and Sensitization
- Drug-Induced Adverse Reactions
- Viral Infections and Vectors
- Contact Dermatitis and Allergies
- Food Allergy and Anaphylaxis Research
- Vector-borne infectious diseases
- Neutropenia and Cancer Infections
- Vector-Borne Animal Diseases
- Acute Myeloid Leukemia Research
- Immunodeficiency and Autoimmune Disorders
- Pulmonary Hypertension Research and Treatments
- Cystic Fibrosis Research Advances
- Inflammatory Myopathies and Dermatomyositis
- Celiac Disease Research and Management
- Antenna Design and Analysis
- Long-Term Effects of COVID-19
- Dermatological and COVID-19 studies
- Occupational and environmental lung diseases
- Inflammasome and immune disorders
- Nematode management and characterization studies
Diakonessenhuis hospital
2020-2025
Elisabeth-TweeSteden Ziekenhuis
2016-2021
University Medical Center Utrecht
2021
Admiraal De Ruyter Ziekenhuis
2019
Reinier de Graaf Hospital
2014
St. Antonius Ziekenhuis
2007-2011
Interstitial Cystitis Association
2009-2011
Bronchoalveolar lavage (BAL) is widely accepted as a key diagnostic procedure in interstitial lung diseases (ILD). We performed study to obtain reference intervals of differential cell patterns BAL fluid with special attention the origin fluid, e.g. bronchial/alveolar, atopy and smoking status age healthy people. bronchoalveolar 55 subjects known (age: 18-64 years, non-smokers/smokers: 34/21) determined counts lymphocyte subsets blood. Moreover, subgroup non-smoking individuals we measured...
Summary The intracellular pathogens Propionibacterium acnes and Mycobacterium tuberculosis have been leading suspects as the cause of sarcoidosis, a systemic disorder characterized by formation non-caseating granulomas. Toll-like receptor (TLR) 2 is important in innate immune response against both pathogens, therefore interest sarcoidosis research. In present study, three single nucleotide polymorphisms one dinucleotide repeat polymorphism TLR-2 gene were genotyped 419 patients, divided into...
Summary Idiopathic pulmonary fibrosis (IPF) is a rapidly progressive interstitial lung disease of unknown aetiology. Interleukin (IL)-1β plays an important role in inflammation and has been associated with fibrotic remodelling. We investigated the balance between IL-1β IL-1 receptor antagonist (IL-1Ra) bronchoalveolar lavage fluid (BALF) serum as well influence genetic variability IL1B IL1RN gene on susceptibility cytokine levels. In 77 IPF patients 349 healthy controls, single nucleotide...
The aim of this verification study was to compare the QuantiFERON®-TB Gold Plus (QFT-Plus) In Tube (QFT-GIT). new QFT-Plus test contains an extra antigen tube which, according manufacturer additionally elicits a CD8+ T-cell response above CD4+ response. We assessed value in detecting recent latent tuberculosis infections.Between May 2015 and December 2016, 1031 subjects underwent QFT-GIT test. Overall agreement between both tests performance for different indications and/or immune states...
Idiopathic inflammatory myopathies (IIM) are a heterogeneous group of connective tissue diseases, collectively known as myositis. Diagnosis IIM is challenging while timely recognition an utter importance considering treatment options and otherwise irreversible (severe) long-term clinical complications. With the EULAR/ACR classification criteria (2017) considerable advancement has been made in diagnostic workup IIM. While these take into account parameters well presence one autoantibody,...
Diagnosis of type I hypersensitivity is based on anamnesis, provocation as well blood- and skin testing. Multiplex specific IgE (sIgE) testing enables determination sIgE antibodies against multiple recombinant or purified natural allergen components. The aim this study was to evaluate the performance novel ALEX2® (Allergy Explorer, ALEX2 test introduced market November 2019) multiplex platform compare it with ImmunoCAP ISAC® system.Serum samples 49 patients, routinely determined ISAC, were...
Sarcoidosis is a granulomatous systemic disorder most often affecting the lung. Pulmonary fibrosis develops in approximately 10%–15% of patients with sarcoidosis. The human gene GREM1 encodes gremlin, member bone morphogenetic protein antagonist family. Bone proteins are essential for maintenance tissue homeostasis and regeneration after injury. We examined associations between genetic variation pulmonary disease outcome Four common tag single nucleotide polymorphisms spanning were genotyped...
α-Gal syndrome (AGS) is an emerging tick-borne disease characterised by the development of specific immunoglobulin E (sIgE) antibodies against galactose-α-1,3-galactose (α-gal), a carbohydrate found in most mammalian species, but not humans. AGS occurs individuals with history tick bites. Currently, no data exist on α-gal sIgE seroprevalence Dutch population, including those bites or erythema migrans (EM). Therefore, this study aimed to determine rate assess seroconversion after bites, and...
Cellular immune responses are of pivotal importance to understand SARS-CoV-2 pathogenicity. Using an enzyme-linked immunosorbent spot (ELISpot) interferon-γ release assay with wild-type spike, membrane and nucleocapsid peptide pools, we longitudinally characterized functional specific T-cell in a cohort patients mild, moderate severe COVID-19. All were included before emergence the Omicron (B.1.1.529) variant. Our most important finding was impaired development early IFN-γ-secreting...
Defining immune correlates of disease severity is important to better understand the immunopathogenesis in COVID-19. Here we made use a protein microarray platform detect IgG- and IgA-reactive antibodies sera saliva respectively, assess cross-reactivity between SARS-CoV-2 endemic coronaviruses (eCoVs). IgG responses against full spike, but not S1 subunit, were significantly higher convalescent patients with severe compared mild healthy controls. In addition, detected reactivity secretory IgA...
Summary Lymphocytes play a crucial role in lung inflammation. Different interstitial diseases may show distinct lymphocyte activation profiles. The aim of this study was to examine the expression variety markers on T subsets from blood and bronchoalveolar lavage fluid (BALF) patients with different granulomatous healthy controls. Bronchoalveolar cells 23 sarcoidosis patients, seven hypersensitivity pneumonitis 24 controls were analysed. Lymphocyte status determined by flow cytometry. stained...
Infection with the novel pandemic SARS-CoV-2 virus has been shown to elicit a cross-reactive immune response that could lead back-boost of memory recall previously encountered seasonal (endemic) coronaviruses (eCoVs). Whether this is associated fatal clinical outcome in patients severe COVID-19 remains unclear. In cohort hospitalized patients, we have heterologous responses eCoVs can be detected COVID-19. Here, report disease decreased neutralizing antibody titers at hospital admission,...
To date, diagnosing food allergies in children still presents a diagnostic dilemma, leading to uncertainty concerning the definite diagnosis of peanut allergy, as well need for strict diets and potential adrenalin auto-injectors. This particular is thought contribute lower quality life. In process double-blind challenges are considered gold standard, but they time-consuming potentially hazardous. Other tests have been extensively studied among these component-resolved diagnostics appeared...
Diagnosis of Lyme borreliosis (LB) relies on clinical symptoms and detection Borrelia-specific antibodies. Guidelines recommend a two-tier testing (TTT) strategy for disseminated LB: serological screening with sensitive enzyme immunoassay (EIA) confirmation specific immunoblot. Searching the most approach, this retrospective study evaluated standard (STTT) modified (MTTT) strategies using well-defined population. Cases included patients active neuroborreliosis (LNB; n = 29) or arthritis (LA;...
Recent studies have shown elevated levels of the B-cell chemokine (C-X-C motif) ligand 13 (CXCL13) in cerebrospinal fluid (CSF) patients with early Lyme neuroborreliosis (LNB). In this retrospective study, we evaluated diagnostic performance Quantikine CXCL13 enzyme-linked immunosorbent assay (ELISA) (R&D Systems, Inc., MN, USA) and recom Bead (Mikrogen, Neuried, Germany) for detection CSF.
Laboratory diagnosis of Lyme neuroborreliosis (LNB) is challenging, and validated diagnostic algorithms are lacking. Therefore, this retrospective cross-sectional study aimed to compare the performance seven commercial antibody assays for LNB diagnosis. Random forest (RF) modeling was conducted investigate whether using could be improved by including several routine cerebrospinal fluid (CSF) parameters (i.e., leukocyte count, total protein, blood-CSF barrier functionality, intrathecal...
Severe acute respiratory syndrome coronavirus 2 infection after disease 2019 vaccination raises concerns about the emergence of vaccine escape variants. Here we characterize 14 breakthrough infections among 5860 fully vaccinated Dutch health care workers ≥14 days final dose with either BNT162b2, mRNA-1273, or Ad26.COV2.S. These presented regular B.1.1.7 (Alpha) and B.1.617.2 (Delta) variants high viral loads, despite normal vaccine-induced B- T-cell immune responses detected by live virus...