A5061203511- Extracellular vesicles in disease
- MicroRNA in disease regulation
- Osteoarthritis Treatment and Mechanisms
- Wnt/β-catenin signaling in development and cancer
- Mesenchymal stem cell research
- Circular RNAs in diseases
- Autophagy in Disease and Therapy
- Cell Adhesion Molecules Research
- Endoplasmic Reticulum Stress and Disease
- FOXO transcription factor regulation
- Cancer Cells and Metastasis
- Cellular transport and secretion
- Genetics and Neurodevelopmental Disorders
- Genetics, Aging, and Longevity in Model Organisms
- Hematopoietic Stem Cell Transplantation
- Chemokine receptors and signaling
- Amyotrophic Lateral Sclerosis Research
- Thermal Regulation in Medicine
- Knee injuries and reconstruction techniques
- Connective Tissue Growth Factor Research
- Blood transfusion and management
- Lipid metabolism and biosynthesis
- Cancer-related gene regulation
- Hippo pathway signaling and YAP/TAZ
- RNA Research and Splicing
University Medical Center Utrecht
2013-2024
Biomedical Primate Research Centre
2022-2024
Utrecht University
2017-2020
Royal Netherlands Academy of Arts and Sciences
2009-2014
University of Amsterdam
2007-2008
Sanquin
2006-2007
Academic Medical Center
2007
Hubrecht Institute for Developmental Biology and Stem Cell Research
2007
Extracellular vesicles (EVs), such as exosomes and microvesicles, are released by different cell types participate in physiological pathophysiological processes. EVs mediate intercellular communication cell‐derived extracellular signalling organelles that transmit specific information from their of origin to target cells. As a result these properties, defined may serve novel tools for various therapeutic approaches, including (a) anti‐tumour therapy, (b) pathogen vaccination, (c)...
The release of RNA-containing extracellular vesicles (EV) into the milieu has been demonstrated in a multitude different vitro cell systems and variety body fluids. EV are limelight for their capacity to communicate genetically encoded messages other cells, suitability as candidate biomarkers diseases, use therapeutic agents. Although EV-RNA attracted enormous interest from basic researchers, clinicians, industry, we currently have limited knowledge on which mechanisms drive regulate RNA...
Osteoarthritis (OA) is a rheumatic disease leading to chronic pain and disability with no effective treatment available.Recently, allogeneic human mesenchymal stromal/stem cells (MSC) entered clinical trials as novel therapy for OA.Increasing evidence suggests that therapeutic efficacy of MSC depends on paracrine signalling.Here we investigated the role extracellular vesicles (EVs) secreted by bone marrow derived (BMMSC) in OA cartilage repair.Methods: To test effect BMMSC-EVs inflammation,...
Bone remodeling is a continuous physiological process that requires constant generation of new osteoblasts from mesenchymal stem cells (MSCs). Differentiation MSCs to osteoblast metabolic switch glycolysis increased mitochondrial respiration ensure the sufficient energy supply complete this process. As consequence metabolism, levels endogenous reactive oxygen species (ROS) rise. In current study we analyzed role forkhead box O3 (FOXO3) in control ROS human (hMSCs) during osteogenic...
Abstract Extravasation of leukocytes is a crucial process in the immunological defense. In response to local concentration chemokines, circulating adhere and migrate across vascular endothelium toward inflamed tissue. The small guanosinetriphosphatase Rap1 plays an important role regulation leukocyte adhesion, polarization, chemotaxis. We investigated guanine nucleotide exchange protein for directly activated by cAMP (Epac1) adhesion chemotaxis promonocytic cell line primary monocytes. found...
Abstract Background Mesenchymal stromal/stem cells (MSCs) and MSC-derived extracellular vesicles (MSC-EVs) hold promise as a disease modifying treatment in osteoarthritis (OA). Obesity, its associated inflammation, contribute to OA development metabolic represents specific significant group of the patient population. Given their immunomodulatory properties, MSC MSC-EVs are especially interesting for this patients therapeutic option. Here, we were first compare efficacy MSCs mild model taking...
The migration of leukocytes across the vascular endothelium is crucial for immunosurveillance as well inflammatory responses. Uncontrolled leukocyte transendothelial results in pathologies such asthma, rheumatoid arthritis, and atherosclerosis. molecular mechanisms that regulate involve signaling downstream intracellular messengers cAMP, calcium, phosphoinositol lipids, or reactive oxygen species. Among these, cAMP particularly intriguing because it generated both endothelial cells regulates...
Abstract Background Allogeneic hematopoietic stem cell transplantation (HSCT) is a curative treatment for leukemia and range of non-malignant disorders. The success the therapy hampered by occurrence acute graft-versus-host disease (aGvHD); an inflammatory response damaging recipient organs, with gut, liver, skin being most susceptible. Intestinal GvHD injury often life-threatening complication in patients unresponsive to steroid treatment. mesenchymal stromal/stem (MSC) infusions are...
Cadherin-mediated cell-cell adhesion controls the morphology and function of epithelial cells is a critical component pathology chronic inflammatory disorders. Dynamic interactions between cadherins actin cytoskeleton are required for stable contact. Besides actin, microtubules also target intercellular, cadherin-based junctions contribute to their formation stability. Here, we studied role in conjunction with Rho-like GTPases regulation lung barrier using real-time monitoring...
Abstract Amyotrophic lateral sclerosis (ALS) is a progressive neurodegenerative disorder with lifetime risk of 1:400, primarily affecting upper and lower motor neurons. Unfortunately, there are only two drugs approved to treat ALS, which increase patient survival by few months. This highlights the urgent need for developments new ALS modifying therapies, have been hampered high failure rate drug candidates during clinical trials. Stem cell therapy one promising approaches that in development...
<title>Abstract</title> Background Allogenic hematopoietic stem cell transplantation (HSCT) is a curative treatment for leukemia and range of non-malignant disorders. The success the therapy hampered by occurrence acute graft-versus-host disease (aGvHD); an inflammatory response damaging recipient organs, with gut, liver, skin being most susceptible. Intestinal GvHD injury often life-threatening complication in patients unresponsive to steroid treatment. Second-line available therapies are...