A5032653169- Molecular Biology Techniques and Applications
- MicroRNA in disease regulation
- Extracellular vesicles in disease
- Reproductive Physiology in Livestock
- Cancer-related molecular mechanisms research
- RNA Research and Splicing
- Milk Quality and Mastitis in Dairy Cows
- Genetic and phenotypic traits in livestock
- Growth Hormone and Insulin-like Growth Factors
- Gene expression and cancer classification
- Adipose Tissue and Metabolism
- Viral gastroenteritis research and epidemiology
- Circular RNAs in diseases
- Clostridium difficile and Clostridium perfringens research
- Estrogen and related hormone effects
- Reproductive Biology and Fertility
- Pharmacological Effects and Assays
- Probiotics and Fermented Foods
- RNA modifications and cancer
- Infant Nutrition and Health
- Reproductive System and Pregnancy
- Microbial infections and disease research
- Animal Nutrition and Physiology
- Trace Elements in Health
- Hormonal and reproductive studies
Technical University of Munich
2016-2025
Karolinska Institutet
2023
University Hospital Augsburg
2023
Universität Bayern
2021
Federal Office for Radiation Protection
2020
Weihenstephan-Triesdorf University of Applied Sciences
2009-2019
Ludwig-Maximilians-Universität München
2003-2019
Committee on Publication Ethics
2019
Leibniz-Institute for Food Systems Biology at the Technical University of Munich
2005-2011
Bayerisches Landesamt für Umwelt
2011
Use of the real-time polymerase chain reaction (PCR) to amplify cDNA products reverse transcribed from mRNA is on way becoming a routine tool in molecular biology study low abundance gene expression. Real-time PCR easy perform, provides necessary accuracy and produces reliable as well rapid quantification results. But accurate nucleic acids requires reproducible methodology an adequate mathematical model for data analysis. This enters into particular topics relative RT–PCR target transcript...
Background: Currently, a lack of consensus exists on how best to perform and interpret quantitative real-time PCR (qPCR) experiments. The problem is exacerbated by sufficient experimental detail in many publications, which impedes reader's ability evaluate critically the quality results presented or repeat Content: Minimum Information for Publication Quantitative Real-Time Experiments (MIQE) guidelines target reliability help ensure integrity scientific literature, promote consistency...
Real-time reverse transcription followed by polymerase chain reaction (RT–PCR) is the most suitable method for detection and quantification of mRNA. It offers high sensitivity, good reproducibility a wide range. Today, relative expression increasingly used, where target gene standardised non-regulated reference gene. Several mathematical algorithms have been developed to compute an ratio, based on real-time PCR efficiency crossing point deviation unknown sample versus control. But all...
There is growing interest in digital PCR (dPCR) because technological progress makes it a practical and increasingly affordable technology. dPCR allows the precise quantification of nucleic acids, facilitating measurement small percentage differences rare variants. may also be more reproducible less susceptible to inhibition than quantitative real-time (qPCR). Consequently, has potential have substantial impact on research as well diagnostic applications. However, with qPCR, ability perform...
The release of RNA-containing extracellular vesicles (EV) into the milieu has been demonstrated in a multitude different vitro cell systems and variety body fluids. EV are limelight for their capacity to communicate genetically encoded messages other cells, suitability as candidate biomarkers diseases, use therapeutic agents. Although EV-RNA attracted enormous interest from basic researchers, clinicians, industry, we currently have limited knowledge on which mechanisms drive regulate RNA...
We have examined the imprecision in estimation of PCR efficiency by means standard curves based on strategic experimental design with large number technical replicates. In particular, how robust this is terms a commonly varying factors: instrument used, replicates performed and effect volume transferred throughout dilution series. used six different qPCR instruments, we 1-16 per concentration tested 2-10 μl analyte transferred, respectively. find that estimated varies significantly across...
Digital PCR (dPCR) has developed considerably since the publication of Minimum Information for Publication Experiments (dMIQE) guidelines in 2013, with advances instrumentation, software, applications, and our understanding its technological potential. Yet these developments also have associated challenges; data analysis steps, including threshold setting, can be difficult preanalytical steps required to purify, concentrate, modify nucleic acids lead measurement error. To assist independent...
Abstract Background RT-qPCR is a sensitive and increasingly used method for gene expression quantification. To normalize measurements between samples, most laboratories use endogenous reference genes as internal controls. There increasing evidence, however, that the of commonly can vary significantly in certain contexts. Results Using Genevestigator database normalized well-annotated microarray experiments, we describe stability characteristics transciptomes several organisms. The results...
ABSTRACT Extracellular vesicles (EVs) are intercellular communicators with key functions in physiological and pathological processes have recently garnered interest because of their diagnostic therapeutic potential. The past decade has brought about the development commercialization a wide array methods to isolate EVs from serum. Which subpopulations captured strongly depends on isolation method, which turn determines how suitable resulting samples for various downstream applications. To...
Adipocyte-derived extracellular vesicles (AdEVs) are membranous nanoparticles that convey communication from adipose tissue to other organs. Here, delineate their role as messengers with glucoregulatory nature, we paired fluorescence AdEV-tracing and SILAC-labeling (phospho)proteomics, revealed AdEVs transfer functional insulinotropic protein cargo into pancreatic β-cells. Upon transfer, AdEV proteins were subjects for phosphorylation, augmented GPCR/cAMP/PKA signaling by increasing total...
In most measurements of gene expression, mRNA is first reverse-transcribed into cDNA. The reverse transcription reaction not very well understood, and it expected to be the uncertain step in expression analysis. It can introduce errors produced by effects secondary tertiary structures, variation priming efficiency, properties transcriptase (1)(2)(3)(4)(5). aim this work was study yield, reproducibility, sensitivity some commercially available transcriptases on low intermediate expressed...
Abstract Our cellular immune system has to cope constantly with foodborne substances that enter the bloodstream postprandially. Here, they may activate leukocytes via specific but yet mostly unknown receptors. Ectopic RNA expression out of gene families chemosensory receptors, i.e., ∼400 ORs, ∼25 TAS2R bitter-taste and TAS1R umami- sweet-taste receptor dimers by which we typically detect substances, been reported in a variety peripheral tissues unrelated olfaction or taste. In present study,...
Abstract Septic shock is a common medical condition with mortality approaching 50% where early diagnosis and treatment are of particular importance for patient survival. Novel biomarkers that serve as prompt indicators sepsis urgently needed. High‐throughput technologies assessing circulating micro RNA s represent an important tool biomarker identification, but the blood‐compartment specificity these mi has not yet been investigated. We characterized profiles from serum exosomes, total blood...
Small RNA-Seq has emerged as a powerful tool in transcriptomics, gene expression profiling and biomarker discovery. Sequencing cell-free nucleic acids, particularly microRNA (miRNA), from liquid biopsies additionally provides exciting possibilities for molecular diagnostics, might help establish disease-specific signatures. The complexity of the small workflow, however, bears challenges biases that researchers need to be aware order generate high-quality data. Rigorous standardization...