A5089834667- Neonatal Respiratory Health Research
- Respiratory viral infections research
- Congenital Diaphragmatic Hernia Studies
- Molecular Biology Techniques and Applications
- Viral gastroenteritis research and epidemiology
- Neuroscience of respiration and sleep
- Pediatric health and respiratory diseases
- Immune Response and Inflammation
- Antimicrobial Peptides and Activities
- Microbial infections and disease research
- Gene expression and cancer classification
- Animal Virus Infections Studies
- Mast cells and histamine
- Prenatal Substance Exposure Effects
- Neuropeptides and Animal Physiology
- Research on Leishmaniasis Studies
- Retinoids in leukemia and cellular processes
- Cell Adhesion Molecules Research
- Virus-based gene therapy research
- Pneumonia and Respiratory Infections
- Toxoplasma gondii Research Studies
- Polyamine Metabolism and Applications
- Computational Drug Discovery Methods
- Trypanosoma species research and implications
- Infant Nutrition and Health
Iowa State University
2011-2024
Boehringer Ingelheim (United States)
2019
Johns Hopkins University
2009
National Animal Disease Center
2002
Agricultural Research Service
2002
The objective of this study was to determine whether vaccination with bacterins commonly used in the USA, when administered at a time typical US protocol, enhances porcine circovirus type 2 (PCV2) replication and incidence severity clinical signs lesions characteristic postweaning multisystemic wasting syndrome (PMWS) conventional pigs. Sixty-one pigs free PCV2 were randomly assigned four groups. Groups 1 ( n = 15) served as sham-inoculated negative controls. 3 14) 4 17) inoculated...
Background Dogs are the predominant domestic reservoir for human L. infantum infection. Zoonotic visceral leishmaniasis (ZVL) is an emerging problem in some U.S. dog breeds, with annual quantitative PCR prevalence of greater than 20% within at-risk Foxhound population. Although classically Leishmania transmitted by infected sand flies and phlebotomine exist United States, means ongoing transmission dogs currently unknown. Possibilities include vertical (transplacental/transmammary)...
Respiratory syncytial virus (RSV) is a common cause of acute lower respiratory disease in infants and young children worldwide. Currently, treatment supportive no vaccines are available. The use newborn lambs to model hRSV infection human may provide valuable tool assess safety efficacy new antiviral drugs vaccines. ALX-0171 trivalent Nanobody targeting the fusion (F) protein its therapeutic potential was evaluated infected with strain RSV followed by daily nebulization for 3 or 5...
ABSTRACT In both dogs and humans Leishmania infantum infection is more prevalent than disease, as often does not equate with clinical disease. Previous studies additively indicate that advanced visceral leishmaniasis characterized by increased production of anti- antibodies, -specific lymphoproliferative unresponsiveness, decreased gamma interferon (IFN-γ) a concomitant increase interleukin-10 (IL-10). order to differentiate versus progressive disease for better prognostication, we...
Abstract Respiratory syncytial virus is a major cause of acute lower respiratory tract infection in young children, immunocompromised adults, and the elderly. Intervention with small-molecule antivirals specific for presents an important therapeutic opportunity, but no such compounds are approved today. Here we report structure JNJ-53718678 bound to fusion (F) protein its prefusion conformation, show that potent nanomolar activity JNJ-53718678, as well preliminary structure–activity...
Our ability to control diseases caused by parasitic nematodes is constrained a limited portfolio of effective drugs and paucity robust tools investigate nematode biology. RNA interference (RNAi) reverse-genetics tool with great potential identify novel drug targets interrogate parasite gene function, but present RNAi protocols for nematodes, which remove the from host execute in vitro, are unreliable inconsistent. We have established an alternative vivo protocol targeting filarial Brugia...
Defensins and surfactant protein A (SP-A) SP-D are antimicrobial components of the pulmonary innate immune system. The purpose this study was to determine extent which parainfluenza type 3 virus infection in neonatal lambs alters expression sheep beta-defensin 1 (SBD-1), SP-A, SP-D, all constitutively transcribed by respiratory epithelia. Parainfluenza viral antigen detected immunohistochemistry (IHC) bronchioles infected days postinoculation at diminished levels 6 postinoculation, but it...
The purpose of this manuscript is to discuss fluorogenic real-time quantitative polymerase chain reaction (qPCR) inhibition and introduce/define a novel Microsoft Excel-based file system which provides way detect avoid inhibition, enables investigators consistently design dynamically-sound, truly LOG-linear qPCR reactions very quickly. problems invention solves are universal all reactions, it performs necessary set-up calculations in about 52 seconds (using pentium 4 processor) for up seven...
Summary Human respiratory syncytial virus (hRSV) is a pneumovirus that causes significant disease in premature and full‐term infants. It was our hypothesis common strain of RSV, A2, would infect, cause pulmonary pathology, alter epithelial innate immune responses neonatal lambs similarly to RSV infection human neonates. Newborn between 2 3 days age were inoculated intrabronchially with A2. The sacrificed at 3, 6, 14 postinoculation. Pulmonary lesions the 6 ‐ day postinoculation group typical...
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Respiratory syncytial virus (RSV) is the leading cause of lower respiratory tract infection in children worldwide. The understanding neonatal RSV pathogenesis depends on using an animal model that reproduces disease. Previous studies from us and others demonstrated lamb resembles human infection. Here, we provide extensive detailed characterization histopathology, viral load, cellular infiltration, cytokine production lungs tracheobronchial lymph nodes lambs inoculated with strain A2 over...
Respiratory syncytial virus (RSV) can cause pulmonary complications in infants, elderly and immunocompromised patients. While two vaccines prophylactic monoclonal antibodies are now available, treatment options still needed. JNJ-7184 is a non-nucleoside inhibitor of the RSV-Large (L) polymerase, displaying potent inhibition both RSV-A -B strains. Resistance selection hydrogen-deuterium exchange experiments suggested binds RSV-L connector domain. prevented RSV replication transcription by...
Factors explaining the greater susceptibility of preterm infants to severe lower respiratory infections with syncytial virus (RSV) remain poorly understood. Fetal/newborn lambs are increasingly appreciated as a model study key elements RSV infection in newborn due similarities lung alveolar development, immune response, and RSV. Previously, our laboratory demonstrated that had elevated viral antigen developed more lesions compared full-term at seven days post-infection. Here, we pathogenesis...
Canine brucellosis is a reportable zoonotic disease that can lead to canine reproductive losses and human infection through contact with infected urine or other genitourinary secretions. Although many locations require testing euthanasia of positive dogs, current diagnosis limited by the time required for seroconversion, example, presence B. canis-specific antibodies. The goal this study was determine diagnostic ability Brucella quantitative polymerase chain reaction (qPCR) assay detect...
Respiratory syncytial virus (RSV) is the most frequent cause of bronchiolitis in infants and children worldwide. There are currently no licensed vaccines or effective antivirals. The lack a vaccine partly due to increased caution following aftermath failed clinical trial formalin-inactivated RSV (FI-RSV) conducted 1960's that led enhanced disease, necessitating hospitalization 80% recipients resulting two fatalities. Perinatal lamb lungs similar size, structure physiology those human...
Rationale Respiratory syncytial virus (RSV) infection in preterm and newborn infants can result severe bronchiolitis hospitalization. The lamb lung has several key features conducive to modeling RSV human infants, including susceptibility strains of such as the A2, Long, Memphis Strain 37 (M37). In this study, kinetics M37 was investigated lambs order better define clinical, viral, physiological, immunological parameters well pathology lesions. Methods Newborn were nebulized with hRSV (6 mL...
Preterm infants have increased susceptibility to severe manifestations of respiratory syncytial virus (RSV) infection. The cause(s) for this age-dependent vulnerability is/are not well-defined, but alterations in innate immune products been implicated. In sheep, RSV disease severity has similar characteristics and sheep several related molecules study during pulmonary infection including surfactant protein A (SP-A), D (SP-D), beta defensin 1 (SBD1), monocyte chemotactic (MCP1), Toll-like...