A5028471651- Genetic Mapping and Diversity in Plants and Animals
- Molecular Biology Techniques and Applications
- Animal Genetics and Reproduction
- Genetic and phenotypic traits in livestock
- Reproductive Biology and Fertility
- Milk Quality and Mastitis in Dairy Cows
- RNA Research and Splicing
- Prion Diseases and Protein Misfolding
- Neurological diseases and metabolism
- Reproductive Physiology in Livestock
- Animal Nutrition and Physiology
- Genetic Neurodegenerative Diseases
- Trace Elements in Health
- RNA regulation and disease
- Immune Response and Inflammation
- Cancer-related molecular mechanisms research
- Infant Nutrition and Health
- RNA modifications and cancer
- Adipose Tissue and Metabolism
- Virus-based gene therapy research
- Escherichia coli research studies
- Peroxisome Proliferator-Activated Receptors
- RNA and protein synthesis mechanisms
- Genetics and Neurodevelopmental Disorders
- Veterinary Oncology Research
Ghent University Hospital
1986-2025
Ghent University
2015-2024
Instituut voor Landbouw en Visserijonderzoek
2015
Veterinary Research Institute
2005
Centre Hospitalier Universitaire de Saint-Pierre
2002
Real-time quantitative PCR is a sensitive and very efficient technique to examine gene transcription patterns in preimplantation embryos, order gain information about embryo development optimize assisted reproductive technologies. Critical the successful application of real-time careful assay design, reaction optimization validation maximize sensitivity accuracy. In most studies published GAPD, ACTB or 18S rRNA have been used as single reference without prior verification their expression...
Abstract Background An essential part of using real-time RT-PCR is that expression results have to be normalized before any conclusions can drawn. This done by one or multiple, validated reference genes, depending on the desired accuracy results. In pig however, very little information available stability genes. The aim this study was therefore develop a new set genes which used for normalization mRNA data expressed in porcine backfat and longissimus dorsi muscle, both representing an...
Retrotransposons are transposable elements that insert extra copies of themselves throughout the genome via an RNA intermediate using a 'copy and paste' mechanism. They account for more than 44% bovine have been reported to be functional, especially during preimplantation embryo development. In present study, we tested whether high oxygen tension (20% O2) influences global DNA methylation analysed by immunofluorescence staining developing embryos this has effect on expression some selected...
Since the development of in vitro embryo production cattle, different supplements have been added to culture media support development, with serum being most popular. However, addition during can induce high birthweights and low viability calves (Large Offspring Syndrome). Analysis global gene expression bovine embryos produced under conditions provide valuable information optimize for production. We used RNA sequencing examine effect production, either serum-containing or serum-free media,...
Real-time quantitative PCR can be a very powerful and accurate technique to examine gene transcription patterns in different biological conditions. One of the critical steps comparing profiles is normalisation. In most studies published on real-time horses, normalisation occurred against only one reference gene, usually GAPDH or ACTB, without validation its expression stability. This might result unreliable conclusions, because it has been demonstrated that levels so called "housekeeping...
Equine sarcoids, the most common skin tumours in horses, are induced by bovine papillomavirus (BPV). Their clinical appearance varies from small stable patches to aggressively growing masses. Differences BPV load and mRNA expression Ki67 p53 immunostaining among four types (fibroblastic, occult, nodular verrucous sarcoids) were evaluated test hypothesis that behaviour of equine sarcoids correlates with activity. Viral E2, E5, E6 E7 genes determined using quantitative real-time PCR. The...
Neonates with intrauterine growth restriction (IUGR) show lower efficiency of nutrient utilization compared to normal birth weight (NBW) newborns. This study was conducted using neonatal piglets as a model test the hypothesis that IUGR affects intestinal barrier function, structure, and antioxidant system development during suckling period. The small mucosae were obtained from NBW littermates in period (d 0, 3, 8, 19 postnatal). epithelial function assessed by FITC-dextran 4 (FD4)...
Objectives: Germline genetic testing is increasingly being integrated into pediatric oncology and a large number of families are interested. Current research on the psychological impact germline limited by main focus individual outcomes in parents or children little known about its at family level. Our study addresses that limitation exploring parents’ lived experiences how their family—as whole—is affected for cancer predisposition. Methods: In six who opted context predisposition, both ill...
Abstract Background Normal preimplantation embryo development encompasses a series of events including first cleavage division, activation the embryonic genome, compaction and blastocyst formation. First lineage differentiation starts at stage with formation trophectoderm inner cell mass. The main objective this study was detection, identification expression analysis genes associated in order to help us better understand process. This information could lead improvements vitro production...
Mammalian blastocyst formation is characterized by two lineage segregations resulting in the of trophectoderm, hypoblast, and epiblast cell lineages. Cell fate determination during these early associated with changes expression specific transcription factors. In addition to factor-based control, it has become clear that also microRNAs (miRNAs) play an important role post-transcriptional regulation pluripotency differentiation. To elucidate miRNAs segregation, we compared miRNA bovine...
Although the equine oviduct clearly affects early embryo development and selective transport of embryos through indicates a reciprocal interaction, influence on gene expression in remains to be determined horse. The aim this study was examine by means RNA sequencing. Four days after ovulation, epithelial cells ipsilateral contralateral ovulation side from five cyclic pregnant mares were collected oviduct. extracted, samples sequenced, data analysis performed determine differentially...
The possible impact of natural heat stress on animal fertility is currently a major concern for breeding companies. Here, we aimed to address this by determining the effects Holstein bulls located in Netherlands. Semen samples were collected from six at two locations March 2016 (low temperature-humidity index (THI) group; maximum THI 51.8 and 55 their respective locations) or August (high 77.9 80.5 during meiotic spermiogenic stages spermatogenesis, 42 14 days prior semen collection). effect...
Abstract Background Weaning is a critical phase in the pigs’ life and gut health might be compromised. Gluconic acid was shown to poorly absorbed but readily fermented butyrate which turn can improve function. Hence, total of 144 weaning pigs were fed experimental diets for 42 days. Three treatments replicated 8 pens with 6 piglets each: control; low dietary dose gluconic acid, 9 g/kg; high 18 g/kg. After 21 days, one piglet from each pen sampled blood haematology biochemistry, fore- hindgut...
F4 enterotoxigenic Escherichia coli (F4 ETEC) are an important cause of diarrhea in neonatal and newly-weaned pigs. Based on the predicted differential O-glycosylation patterns 2 MUC13 variants (MUC13A MUC13B) F4ac ETEC susceptible resistant pigs, gene was recently proposed as causal for susceptibility. Because absence Western blot from brush border membrane vesicles F4ab/acR+ pigs attachment to immunoprecipitated could not support this hypothesis, a new GWAS study performed using 52...
Abstract Host-parasite co-evolution history is lacking when parasites switch to novel hosts. This was the case for Western honey bees ( Apis mellifera ) ectoparasitic mite, Varroa destructor , switched hosts from Eastern cerana ). mite has since become most severe biological threat A. worldwide. However, some populations are known survive infestations, largely by suppressing population growth. One mechanism suppressed reproduction (SMR), but underlying genetics poorly understood. Here, we...
Deoxynivalenol (DON) is a mycotoxin produced by Fusarium species in the field, commonly found cereal grains, which negatively affects performances and health of animals. Mycotoxin binders are supposed to reduce toxicity mycotoxins. The effect binder (containing acid-activated bentonite, clinoptilolite, yeast cell walls organic acids) on growth performance gut was studied. Hundred twenty weaning piglets were allocated 4 treatments, with 5 pens 6 each, arranged 2 × factorial design: control...
Abstract Background Early-life antibiotic administration is known to affect gut microbiota and host adiposity, but the effects of exposure on skeletal muscle properties remain unknown. The present study evaluated changes in including myofiber characteristics composition, as well intramuscular fat (IMF) content piglets when exposed a tylosin-containing diet. Results A total 18 (28 days age) were randomly allocated into two groups: control basal diet (Control) Control + 100 mg tylosin...
Complexes between mRNA and GL67:DOPE:DMPE-PEG5000 (GL67) liposomes were formulated characterized. Subsequently, the in vitro vivo expression characteristics of mRNA/GL67 complexes pDNA/GL67 complexes, each produced at their optimal ratio, compared respiratory cells. Transfection A549 cells with resulted a much faster than after transfection complexes. The percentage GFP-positive pDNA peaked 8 24 h, respectively. At these time points was two times higher transfection. Furthermore, efficacy...