A5062533454- Genetic Mapping and Diversity in Plants and Animals
- Genetic and phenotypic traits in livestock
- Molecular Biology Techniques and Applications
- Animal Genetics and Reproduction
- Animal Nutrition and Physiology
- Meat and Animal Product Quality
- Wheat and Barley Genetics and Pathology
- Glycosylation and Glycoproteins Research
- Protein Interaction Studies and Fluorescence Analysis
- RNA Research and Splicing
- Cancer-related molecular mechanisms research
- Livestock and Poultry Management
- Aquaculture disease management and microbiota
- Genetics and Plant Breeding
- Microbial infections and disease research
- Toxin Mechanisms and Immunotoxins
- RNA and protein synthesis mechanisms
- Animal Virus Infections Studies
- Identification and Quantification in Food
- Genomics and Chromatin Dynamics
- Veterinary Equine Medical Research
- Physiological and biochemical adaptations
- GABA and Rice Research
- Aquaculture Nutrition and Growth
- RNA modifications and cancer
Czech Academy of Sciences, Institute of Animal Physiology and Genetics
2009-2022
Czech University of Life Sciences Prague
2020-2022
Czech Academy of Sciences
1995-2010
University of Bologna
1998
Mendel University in Brno
1997
Swedish University of Agricultural Sciences
1987-1988
Czech Academy of Sciences, Institute of Molecular Genetics
1985
Institute of Animal Physiology of the Slovak Academy of Sciences
1974-1975
Czech Academy of Sciences, Institute of Physiology
1973
Summary Three informative pig F 2 families based on European Wild Boar (W), Meishan (M) and Pietrain (P) crosses have been used for genome‐wide linkage quantitative trait loci (QTL) analysis. Altogether 129 microsatellites, 56 type I 46 definitions (specific to growth, fattening, fat deposition, muscling, meat quality, stress resistance body conformation) were included in the study. In maps of M × P, W P families, average spacing markers 18.4, 19.7 18.8 cM, numbers meioses 582, 534 625,...
Abstract Background Independent studies have shown that several single nucleotide polymorphisms (SNP) in the human FTO ( fat mass and obesity associated ) gene are with obesity. SNP also been identified pig gene, among which some selected fat-deposition traits F 2 crosses commercial populations. In this study, using both populations an experimental Meishan × Pietrain population, we investigated association between one growth carcass traits. Association analyses were performed polymorphism...
Summary Linkage maps have been constructed for Sus scrofa chromosome 6 (SSC6) based on 17 markers, of which 10 were used in all three F 2 families Wild Boar (W), Meishan (M) and Pietrain (P) crosses. The coverage the linkage SSC6 was almost complete. All loci ordered identically families, but two intervals ( RYR1‐A1BG‐EAH , S0146‐S0003‐SW824 ) differed from published maps. Major quantitative trait (QTLs) meat quality, stress resistance carcass composition explaining up to 59% phenotypic...
Summary Linkage maps of Sus scrofa chromosome 4 (SSC4) based on 19 markers were similar in lengths for three F 2 ‐families [Wild Boar (W), Meishan (M) and Pietrain (P) crosses] agreement with the previously published maps. Quantitative trait loci (QTLs) SSC4 affect mainly muscle fat mass. Compared others, alleles associated longer carcass, higher body weight meat content. In all families, profiles ratio values these traits showed peaks close to positions candidate genes V‐ATPase , ATP1A2...
Over the years, researchers have used presumptively neutral molecular variation to infer origins of current species' distributions in northern latitudes (especially Europe). However, several reported examples genic and chromosomal replacements suggest that end-glacial colonizations particular areas may involved genetic input from different source populations at times, coupled with competition selection. We investigate functional consequences differences between two bank vole (Clethrionomys...
Summary Linkage maps of Sus scrofa chromosome 7 (SSC7) for three informative F 2 families, based on Wild Boar (W), Meishan (M) and Pietrain (P) crosses, were constructed using 16 marker loci. Maps consistent the except order markers PI2‐PO1A‐S0212 in W × M family which was inverted P families. Important quantitative trait loci (QTLs) localized region SLA complex These explained up to 16% phenotypic variance body conformation, fat deposition muscling. In family, minor QTLs mapped outside...
The single-banding components of bovine transferrin from animals homozygous for the four variants found in U.K. were isolated. Sedimentation equilibrium ultracentrifugation and sodium dodecyl sulphate-polyacrylamide-gel electrophoresis showed that bands a single variant have molecular weights 77500 73300 respectively. different show no evidence size heterogeneity or low-molecular-weight peptides being split off after reduction 6m-guanidine hydrochloride. two slower variant, which both...
QTL affecting fat deposition related performance traits have been considered in several studies and mapped on numerous porcine chromosomes. However, activity of specific enzymes, protein content cell structure tissue probably depend a smaller number genes than to carcass. Thus, this work metabolic cytological features back were investigated genome-wide analysis. similarities differences examined between three F2 crosses, male female animals.A total 966 animals originating from crosses...
Summary Homozygous transferrins of adult cattle are made up two strong pairs and one weak pair protein bands on starch gel electrophoresis. Foetal have only the slower band each with fastest three being much stronger than in type. Before term second begins to develop at same time becomes weaker – attaining type by or soon after. The ai protein, which is present early foetal life almost disappears 250 days embryonic development, shows individual variation. Its relationship fetuin discussed.
Summary Linkage maps of Sus scrofa chromosome 5 (SSC5) were constructed using up to 10 markers in three informative F 2 families based on Wild Boar (W), Meishan (M) and Pietrain (P) crosses. The map lengths differed among the families. In M × P family, MYF5 locus was linkage mapped between SW995 SW967 . Relatively few quantitative trait loci (QTLs) observed SSC5 explaining a maximum 5.8% phenotypic variance generation. presence position QTLs not consistent QTL allele effects inferior...
Summary Linkage and QTL maps of Sus scrofa chromosome 18 (SSC18) were generated using five markers in three F 2 families based on Wild Boar (W), Pietrain (P) Meishan crosses. The linkage similar for the confirm published map. Quantitative trait loci (QTLs) meat content, fat‐to‐meat ratio, weight liver food conversion ratio mapped W × M family. A pH value 24 h after slaughter Musculus semimembranosus was found P carcass content family localized near leptin gene ( LEP ).
Summary For Sus scrofa chromosome 13 (SSC13) eleven markers were genotyped to generate linkage and QTL maps in three F 2 families based on Wild Boar (W), Meishan (M) Pietrain (P) crosses. Results of similar agreed with published maps. Quantitative trait loci (QTLs) for body weights, meat conductivity fat deposition mapped the M × P family interval S0076–S0068 (40–80 cM) near locus SW38 (120–150 cM). QTLs same traits observed W different intervals explaining up 5.3% phenotypic variance. No...
Three serotransferrin variants Tf 2a, 4b and 5b were isolated in an homogeneous form from a preparation of homozygous horse 0. On the basis results concerning molecular mass determination carbohydrate analysis, it is concluded that variant 2a contains only one glycan while contain two glycans. The structure all glycans has been established by combining methylation spectrometry 400‐MHz 1 H‐NMR spectroscopy. From obtained results, appears are, like human serotransferrin, N ‐acetyllactosaminic...
Of eight monoclonal antibodies raised against human transferrin, one (H.TF-14) cross reacted with pig and rabbit transferrins (H.TF-1) showed cross-reactivity horse dog transferrins. While exhibited the same patterns of binding to MOLT-3 cell receptors as transferrins, mouse was weaker bovine carp gave entirely negative results. The results these competitive experiments were confirmed by a biological test in which transferrin had no effect on growth cells when added serum-free medium....
Summary. Pig, horse and dog plasma proteins, separated by horizontal polyacrylamide gel electrophoresis (pH 9.0) electrophoretically transferred to nitrocellulose membranes, were tested for cross‐reaction with antiserum human α 1 B‐glycoprotein (α B). The results showed that one previously reported polymorphic postalbumin in each of these species (pig PO2, Xk Pa protein) was homologous B. In the light known genetic linkages species, this implied: (1) B gene is close linked Phi, Pgd Hal...