A5048685893- Reproductive Biology and Fertility
- Sperm and Testicular Function
- Reproductive Physiology in Livestock
- Animal Genetics and Reproduction
- Genetic and phenotypic traits in livestock
- Ovarian function and disorders
- Veterinary Medicine and Surgery
- Pluripotent Stem Cells Research
- Vector-Borne Animal Diseases
- Molecular Biology Techniques and Applications
- Animal Disease Management and Epidemiology
- Reproductive Health and Technologies
- Animal Behavior and Welfare Studies
- Effects of Environmental Stressors on Livestock
- Tissue Engineering and Regenerative Medicine
- Animal Virus Infections Studies
- Seed Germination and Physiology
- Urological Disorders and Treatments
- Veterinary Equine Medical Research
- MicroRNA in disease regulation
- Virus-based gene therapy research
- Reproductive System and Pregnancy
- Mesenchymal stem cell research
- Reproductive biology and impacts on aquatic species
- Renal and related cancers
Ghent University
2016-2025
Ghent University Hospital
2005-2024
KU Leuven
2023
Cordoba University
2023
Instituut voor Landbouw en Visserijonderzoek
1997-2018
Koninklijke Nederlandse Maatschappij voor Diergeneeskunde
2011-2016
Utrecht University
2015
Salisbury University
1996-2003
In this study concentration and composition of non-esterified fatty acids (NEFA) in follicular fluid (FF) high-yielding dairy cows were determined during the period negative energy balance (NEB) early post partum. NEFA then added vitro maturation at concentrations measured previously FF to evaluate their effect on oocyte's developmental competence. At 16 44 days partum, dominant follicle blood collected from nine cows. Samples analysed for composition. (0.2-0.6 mmol/l) NEB remained +/- 40%...
Real-time quantitative PCR is a sensitive and very efficient technique to examine gene transcription patterns in preimplantation embryos, order gain information about embryo development optimize assisted reproductive technologies. Critical the successful application of real-time careful assay design, reaction optimization validation maximize sensitivity accuracy. In most studies published GAPD, ACTB or 18S rRNA have been used as single reference without prior verification their expression...
Noninvasive measurements of bovine embryo quality, such as timing cleavage, morula morphology, blastocyst formation, and hatching ability, were linked with the number inner cell mass (ICM) cells trophectoderm (TE) resulting embryos. First, it was confirmed that fast-cleaving embryos proved to have significantly higher chances reach advanced developmental stages vs. intermediate slow cleavers (P = 0.01). They also showed less fragmentation at stage, implying presence more excellent morulae...
Abstract Human embryonic stem cells (hESCs) closely resemble mouse epiblast exhibiting primed pluripotency unlike ESCs (mESCs), which acquire a naïve pluripotent state. Efforts have been made to trigger in hESCs for subsequent unbiased lineage-specific differentiation, common conundrum faced by due heterogeneity gene expression existing within and between hESC lines. This required either ectopic of genes such as NANOG KLF2 or inclusion multiple pluripotency-associated factors. We report here...
Dramatic genome dynamics, such as chromosome instability, contribute to the remarkable genomic heterogeneity among blastomeres comprising a single embryo during human preimplantation development. This heterogeneity, when compatible with life, manifests constitutional mosaicism, chimerism, and mixoploidy in live-born individuals. Chimerism are defined by presence of cell lineages different parental genomes or ploidy states individual, respectively. Our knowledge their mechanistic origin...
Is the rate and nature of chromosome instability (CIN) similar between bovine in vivo-derived vitro-cultured cleavage-stage embryos? There is a major difference regarding stability embryos, as CIN significantly lower embryos compared to embryos. common during vitro embryogenesis associated with early embryonic loss humans, but vivo-conceived remains largely unknown. Because human vivo preimplantation are not accessible, (Bos taurus) were used study vivo. Five young, healthy, cycling Holstein...
SignificanceHatching from the zona pellucida is a prerequisite for embryo implantation and less likely to occur in vitro reasons unknown. Extracellular vesicles (EVs) are secreted by into culture medium. Yet role that embryonic EVs their cargo microRNAs (miRNAs) play blastocyst hatching has not been elucidated, partially due difficulties of isolating them low amounts Here, we optimized EV-miRNA isolation medium conditioned individually cultured bovine embryos subsequently showed miR-378a-3p,...
Preimplantation development in the bovine embryo was examined by relating occurrence of three morphogenetic processes (compaction, blastulation, and hatching) to timing allocation embryonic cells inner cell mass (ICM) or trophectoderm (TE). Embryos were collected from 26 cows between Days 4 9 postovulation. Compaction started 5 days postovulation at 32-cell stage. Morulae remained firmly compact until seventh cycle almost completed. Blastocyst formation 64- 128-cell stage 6, 7, 8 Hatching...
Data from other laboratories have shown that speed of bovine blastocyst development is higher when Ménézo B2 used for coculture compared to TCM199. It was our purpose investigate whether this early formation also indicative embryo quality by studying the allocation inner cells in embryos generated B2-coculture and TCM199-coculture. For purpose, a differential staining technique used. General similar TCM199- B2-embryos expressed as rate cleavage at day 3 morula-blastocyst 8 (P > 0.05), but...