A5022156516- Reproductive Biology and Fertility
- Reproductive Physiology in Livestock
- Animal Genetics and Reproduction
- Sperm and Testicular Function
- Rabbits: Nutrition, Reproduction, Health
- Renal and related cancers
- CRISPR and Genetic Engineering
- Ovarian function and disorders
- Genetic and phenotypic traits in livestock
- Pluripotent Stem Cells Research
- Assisted Reproductive Technology and Twin Pregnancy
- Molecular Biology Techniques and Applications
- Effects of Environmental Stressors on Livestock
- Reproductive System and Pregnancy
- Prenatal Screening and Diagnostics
- Animal Behavior and Welfare Studies
- Virus-based gene therapy research
- Viral Infectious Diseases and Gene Expression in Insects
- Epigenetics and DNA Methylation
- Genetic Syndromes and Imprinting
- Growth Hormone and Insulin-like Growth Factors
- Transgenic Plants and Applications
- Ruminant Nutrition and Digestive Physiology
- Biochemical Analysis and Sensing Techniques
- Heavy Metal Exposure and Toxicity
University of Veterinary Medicine Vienna
2014-2025
BOKU University
2004-2020
Agriculture and Agri-Food Canada
2019
Université Laval
2019
University of Saskatchewan
2019
Freie Universität Berlin
2019
Instituto de Investigaciones Agropecuarias
2019
Agrobiogen (Germany)
2004-2010
Max Planck Institute for Molecular Genetics
2008
National Agricultural Research Foundation
2005
Understanding gene expression patterns in response to altered environmental conditions at different time points of the preimplantation period would improve our knowledge on regulation embryonic development. Here we aimed examine effect alternative vivo and vitro culture major genome activation (EGA) development transcriptome profile bovine blastocysts. Four blastocyst groups were produced under before or after EGA. Completely vitro- vivo-produced blastocysts used as controls. We compared...
Bovine embryos can be generated by in vitro fertilization or somatic nuclear transfer; however, these differ from their vivo counterparts many aspects and exhibit a higher proportion of developmental abnormalities. Here, we determined for the first time transcriptomes bovine metaphase II oocytes all stages preimplantation developing up to blastocyst using Affymetrix GeneChip Genome Array which examines approximately 23,000 transcripts. The data show that transcribed significantly number...
Abstract Worldwide over 5 million children have been conceived using assisted reproductive technology, and research has concentrated on increasing the likelihood of ongoing pregnancy. However, studies animal models indicated undesirable effects in vitro embryo culture offspring development health. In vivo, oviduct hosts a period which early undergoes complete reprogramming its (epi)genome preparation for reacquisition (epi)genetic marks. We designed an oviduct-on-a-chip platform to better...
This study examined the effect of presence single or multiple embryos on transcriptome bovine oviduct. In experiment 1, cyclic (nonbred, n = 6) and pregnant (artificially inseminated, 11) heifers were slaughtered Day 3 after estrus, ampulla isthmic regions oviduct ipsilateral to corpus luteum separately flushed. Oviductal epithelial cells from isthmus region, in which all oocytes/embryos located, snap-frozen for microarray analysis. 2, divided into (multiple embryo transfer, 10) groups....
Is the rate and nature of chromosome instability (CIN) similar between bovine in vivo-derived vitro-cultured cleavage-stage embryos? There is a major difference regarding stability embryos, as CIN significantly lower embryos compared to embryos. common during vitro embryogenesis associated with early embryonic loss humans, but vivo-conceived remains largely unknown. Because human vivo preimplantation are not accessible, (Bos taurus) were used study vivo. Five young, healthy, cycling Holstein...
Elevated concentrations of circulating progesterone in the immediate postconception period have been associated with an increase embryonic growth rate, interferon-tau production, and pregnancy rate cattle sheep. Much this effect is likely mediated via downstream effects progesterone-induced changes gene expression uterine tissues. Using state-of-the-art endoscopic techniques, study examined elevated on development vitro produced bovine zygotes transferred to oviducts heifers high or normal...
Early embryonic loss and altered gene expression in vitro produced blastocysts are believed to be partly caused by aberrant DNA methylation. However, specific stage which is sensitive culture conditions alter the methylation profile of resulting remained unclear. Therefore, aim this study was investigate effect environment on response blastocysts. For this, embryos cultured until zygote (ZY), 4-cell (4C) or 16-cell (16C) were transferred recipients recovery at day 7 estrous cycle. Another...
In mammals, the reproductive tract plays a crucial role in success of early events and provides an optimal microenvironment for embryonic development. However, changes environment associated with controlled ovarian hyperstimulation influence on embryo transcriptome profile have not been investigated. Therefore, we investigated differences development rate bovine blastocysts developing unstimulated or superovulated heifers. Nineteen Simmental heifers were synchronized, artificially...
Aberrant DNA methylation patterns of genes required for development are common in vitro produced embryos. In this regard, we previously identified altered vivo developed blastocysts from embryos which spent different stages vitro, indicating carryover effects suboptimal culture conditions on epigenetic signatures preimplantation However, responses originated to not fully understood. Therefore, here investigated derived bovine subjected condition before, during or after major embryonic genome...
This review focuses on the key features of development bovine oocyte and embryo, with comparisons developmental characteristics embryos produced in vivo vitro. The is transcriptionally quiescent primordial primary follicle. In secondary follicle transcription initiated a ribosome-synthesizing nucleolus established this cell. Transcription nucleolar activity are enhanced tertiary during growth. When reaches approximately 110 microm diameter, corresponding to about 3 mm ceases inactivated,...
A technique for endoscopic transfer of one-cell, one-two-cell, two-cell or morula stage embryos to the Fallopian tubes rabbits was developed in two phases. Phase I experiments involved 30-65 each nine recipients and resulted pregnancies all animals. After 12 days six were killed they showed an implantation rate 27%. The remaining three continued pregnancy until birth. embryo survival 26%. In a second phase, 10-20 transferred 22 recipient animals, 19 which went on give birth (embryo animals...
The aim was to investigate the effect of lactation on composition pre-ovulatory follicular fluid (FF). Forty in-calf primiparous heifers and 20 maiden were enrolled. Immediately after calving, half cows dried off while remainder milked twice daily. Serum samples collected weekly from two weeks pre- 84 days postpartum (dpp). FF analysed by gas chromatography-mass spectrometry. concentrations non-esterified fatty acids β-hydroxybutyrate higher, glucose, insulin Insulin-like growth factor 1...
Abstract Background Various studies have highlighted significant differences in developmental kinetics and sensitivity to conditions between male female bovine embryos. These are thought be caused part by the sexually dimorphic expression of genes located on sex or autosomal chromosomes. However, little is known about gene patterns embryos at initiation elongation, which one critical stages development. Furthermore, best our knowledge, there no data available relation maternal environmental...
Complex recombinant antibody fragments for modulation of immune function such as tumor cell destruction have emerged at a rapid pace and diverse anticancer strategies are being developed to benefit patients. Despite improvements in molecule design expression systems, the quantity stability, e.g., single-chain antibodies produced culture, is often insufficient treatment human disease, costs scale-up, labor, fermentation facilities prohibitive. The ability yield mg/ml levels scale-up...
Abstract In this study, a combination of RNAi and endoscopic transfer to the oviduct synchronized heifers has been used investigate effect suppression Cx43 E‐cadherin on development, mRNA protein expression bovine blastocysts cultured in vitro or vivo. matured fertilized zygotes were randomly assigned one four groups namely: Connexin43 dsRNA‐injected (n = 790), 775), water‐injected 774), noninjected controls 652). Following 2 days culture, 4‐ 8‐cell stage embryos from each treatment group...
Insulin-like growth factor I (IGF-I) has acute insulin-like metabolic effects and long-term anabolic actions offering a range of important therapeutic applications. To evaluate system for large-scale production this peptide in the mammary glands transgenic livestock, we generated rabbits carrying fusion genes which synthetic DNA coding human IGF-I (hIGF-I) was placed under transcriptional control regulatory elements isolated from bovine αS1-casein (αS1-cas) gene. Western blot analysis milk...
RNA interference (RNAi) has been used for selective degradation of an mRNA transcript or inhibiting its translation to a functional protein in various species. Here, we applied the RNAi approach suppress expression maternal C-mos and embryonic transcripts Oct-4 bovine oocytes embryos respectively, using microinjection sequence-specific double-stranded (dsRNA). For this, 435 bp 341 dsRNA were synthesized microinjected into cytoplasm immature zygotes respectively. In experiment 1, categorized...