A5034027223- Microfluidic and Capillary Electrophoresis Applications
- Innovative Microfluidic and Catalytic Techniques Innovation
- 3D Printing in Biomedical Research
- Microfluidic and Bio-sensing Technologies
- Electrowetting and Microfluidic Technologies
- Reproductive Biology and Fertility
- Spectroscopy Techniques in Biomedical and Chemical Research
- Biosensors and Analytical Detection
- Extracellular vesicles in disease
- Cancer Genomics and Diagnostics
- Protein purification and stability
- Advanced biosensing and bioanalysis techniques
- Fuel Cells and Related Materials
- Additive Manufacturing and 3D Printing Technologies
- Mechanical Circulatory Support Devices
- Bone Tissue Engineering Materials
- Tendon Structure and Treatment
- Sperm and Testicular Function
- Cellular Mechanics and Interactions
- Cell Image Analysis Techniques
- Barrier Structure and Function Studies
- Membrane Separation and Gas Transport
- solar cell performance optimization
- Pesticide Residue Analysis and Safety
- RNA Interference and Gene Delivery
Maastricht University
2019-2024
University of Twente
2014-2021
Inspire
2018
Auburn University
2016
Chan Heart Rhythm Institute
2015
Institute of Nanotechnology
2015
Organs-on-chips (OoCs) are systems containing engineered or natural miniature tissues grown inside microfluidic chips. To better mimic human physiology, the chips designed to control cell microenvironments and maintain tissue-specific functions. Combining advances in tissue engineering microfabrication, OoCs have gained interest as a next-generation experimental platform investigate pathophysiology effect of therapeutics body. There many examples there applications, making it difficult for...
Abstract Worldwide over 5 million children have been conceived using assisted reproductive technology, and research has concentrated on increasing the likelihood of ongoing pregnancy. However, studies animal models indicated undesirable effects in vitro embryo culture offspring development health. In vivo, oviduct hosts a period which early undergoes complete reprogramming its (epi)genome preparation for reacquisition (epi)genetic marks. We designed an oviduct-on-a-chip platform to better...
Here, we report a platform for the specific capture of tdEVs, followed by their <italic>in situ</italic> characterization using SEM, Raman spectroscopy and AFM, at single EV level, in terms size, size distribution chemical fingerprinting.
Abstract Microfluidic systems enable automated and highly parallelized cell culture with low volumes defined liquid dosing. To achieve this, typically integrate all functions into a single, monolithic device as “one size fits all” solution. However, this approach limits the end users’ (re)design flexibility complicates addition of new to system. address challenge, we propose demonstrate modular standardized plug-and-play fluidic circuit board (FCB) for operating microfluidic building blocks...
A microfluidic droplet-storage array that is capable of the continuous operation droplet formation, storing, repositioning, retrieving, injecting and restoring demonstrated. The chip comprised four valve-assisted generators a 3 × 16 array. integrated pneumatically actuated microvalves enable precise control aqueous phase dispensing, as well carrier fluid flow path direction for flexible manipulating water-in-oil droplets in chip. size formed by was validated under various operating...
Self-seeding microwell chips can sort single cells into 6400 wells based on cell size and their identity verified by immunofluorescence staining. Here, we developed a microfluidic device in which these be placed, lysed DNA amplified for further interrogation. Whole blood spiked with MCF7 tumor was passed through the after leukocyte depletion 37% of were identified epithelial adhesion molecule (EpCAM) staining microwells. Identified punched reaction chamber reagents lysis amplification...
A micro-engineered physiological system-tissue barrier chip (MEPS-TBC) has been developed as a highly replicable model of the human blood–brain (BBB) with 3D perivascular space using tunable aspiration technique.
The heterogeneity of tumor cells and their alteration during the course disease urges need for real time characterization individual to improve assessment treatment options. New generations therapies are frequently associated with specific genetic alterations driving determine makeup cells. Here, we present a microfluidic device parallel single cell whole genome amplification (pscWGA) obtain enough copies probe presence targets frequency its occurrence among Individual were first captured...
Tendon is a highly organized tissue that transmits forces between muscle and bone. The architecture of the extracellular matrix tendon, predominantly from collagen type I, important for maintaining tenocyte phenotype function. Therefore, in repair regeneration damaged diseased tendon tissue, it crucial to restore aligned arrangement I fibers original matrix. To this end, novel, user-friendly microfluidic piggyback platform developed allowing controlled patterned formation alignment simply on...
A new microfluidic valve or a “v-type valve” which can be flexibly actuated to focus fluid flow and block specific area of microchannel is demonstrated.
A microfluidic platform or “microfluidic mapper” is demonstrated, which in a single experiment performs 36 parallel biochemical reactions with different combinations of two reagents stepwise concentration gradients. The volume used each individual reaction was nl. With the mapper, we obtained 3D enzyme plot horseradish peroxidase (HRP) Amplex Red (AR) and hydrogen peroxide (H2O2), for ranges 11.7 μM to 100.0 11.1 66.7 AR H2O2, respectively. This system methodology could be as fast analytical...
Microengineering is increasingly being used for controlling the microenvironment of stem cells. Here, a novel method fabricating structures with subcellular dimensions in commonly available thermoplastic poly(methyl methacrylate) (PMMA) shown. Microstructures are produced PMMA substrates using Deep Ultraviolet lithography, and effect different developers described. Microgrooves fabricated neuronal differentiation mouse embryonic cells (mESCs) directly on polymer. The fabrication 3D,...
A microfluidic platform or "microfluidic batch adsorption device" is presented, which performs two sets of 9 parallel protein incubations with/without adsorbent particles to achieve an isotherm a in single experiment. The stepwise concentration gradient target was created by the integration microvalves into device. nanoliter-scale reactor (41 nl) allows about 5000 times reduction sample consumption and fast analysis compared with conventional 96 well plate. reactors as reference reactors,...
A microfluidic chip was designed, prepared and tested for integration with a confocal Raman imaging spectrometer the specific purpose of enabling studies individual biological cells.
Abstract Enthesitis, the inflammation of enthesis, which is point attachment tendons and ligaments to bones, a common musculoskeletal disease. The often originates from fibrocartilage region enthesis as consequence mechanical overuse or ‐load consequently tissue damage. During enthesitis, waves inflammatory cytokines propagate in(to) fibrocartilage, resulting in detrimental, heterotopic bone formation. Understanding human enthesitis its treatment options limited, also because lacking vitro...
A pneumatically operated peristaltic micro pump can play an important role in nanoliter or picoliter scale fluid handling and delivery for biomimetic micro-scale peristalsis system as well biological chemical applications. Here, we first investigate the performance of that are composed micrometer mechanical valve. We examined effect number pumping valves, from two to six, with variations operating frequency. The pumps 200×100 μm2 valve area 100 μm wide 10 deep microchannels were mechanically...
Abstract In this work, alveolar‐like microfluidic devices are studied with a horizontal membrane arrangement that demonstrate great potential as small‐scale blood oxygenator. The design criteria for the fabricated to maximize oxygen saturation level and minimize liquid chamber volume while ensuring physiological flow in order avoid thrombus formation channel blockage during operation. architecture is iteratively modified upon analysis of fluid dynamics by computer modeling. Accordingly, two...
Abstract A microfluidic device for pH gradient chromatofocusing is presented, which performs creation of a micro‐column, generation, and fraction collection in single device. Using sieve micro‐valve, anion exchange particles were packed into microchannel order to realize solid‐phase absorption column. To fractionate proteins according their isoelectric points, elution buffer solutions with stepwise prepared 16 parallel mixing reactors flowed through the wherein protein mixture was previously...
A programmable droplet-based microfluidic serial dilutor platform is presented, which capable of generating a series droplets with the scalable stepwise concentration gradient sample. Sequential dilution target molecule was automatically performed in sub-nanoliter scale by synchronizing peristaltic mixer and valve-assisted droplet generator. The volume dispensed from controlled microvalve operation, enabled to tune various factors. After evaluation efficiency calibration size at different...
Drug resistance is frequently developing during treatment of cancer patients. Intracellular drug uptake one the important characteristics to understand mechanism resistance. However, heterogeneity cells requires investigation at single cell level. Here, we developed a microfluidic device for parallel probing uptake. We combined v-type valve and peristaltic pumping select individual from pool prostate (PC3) place them successively in separate chambers which they were exposed drug. Six...
This paper describes an innovative yet straightforward fabrication technique to create three-dimensional microstructures with controllable tapered geometries by combining conventional photolithography and thermal reflow of photoresist. Positive photoresist-based microchannel structures varying width-to-length ratios were reflowed after their generate funnel curvatures. A polydimethylsiloxane hourglass-shaped array was next cast on these photoresist structures, primary human lung...
Microfluidic devices based on the multilayer soft lithography allow accurate manipulation of liquids, handling reagents at sub-nanoliter level, and performing multiple reactions in parallel processors by adapting micromixers. Here, we have experimentally evaluated compared several designs micromixers operating conditions to find design guidelines for We tested circular, triangular, rectangular mixing loops measured performance according position width valves that drive nanoliters fluids...