A5037959579- 3D Printing in Biomedical Research
- Advanced biosensing and bioanalysis techniques
- Cancer Cells and Metastasis
- Single-cell and spatial transcriptomics
- Nanopore and Nanochannel Transport Studies
- RNA modifications and cancer
- Immune cells in cancer
- Innovative Microfluidic and Catalytic Techniques Innovation
- Microfluidic and Capillary Electrophoresis Applications
- Neuroscience and Neural Engineering
- Monoclonal and Polyclonal Antibodies Research
- Gut microbiota and health
- Lipid Membrane Structure and Behavior
- MicroRNA in disease regulation
- Cancer Research and Treatments
- Nanoparticles: synthesis and applications
- Advanced Biosensing Techniques and Applications
- Gold and Silver Nanoparticles Synthesis and Applications
- Analytical Chemistry and Sensors
- Drug-Induced Ocular Toxicity
- Nanoplatforms for cancer theranostics
- Neuroinflammation and Neurodegeneration Mechanisms
- Ferroptosis and cancer prognosis
- Molecular Biology Techniques and Applications
- Spectroscopy and Quantum Chemical Studies
Advanced Cell Diagnostics (United States)
2023-2024
Georgia Institute of Technology
2018-2024
The Wallace H. Coulter Department of Biomedical Engineering
2022-2024
Emory University
2018
BioSurfaces (United States)
2017
University of Michigan–Ann Arbor
2017
Ann Arbor Center for Independent Living
2017
Korea University
2011
Organs-on-chips (OoCs) are systems containing engineered or natural miniature tissues grown inside microfluidic chips. To better mimic human physiology, the chips designed to control cell microenvironments and maintain tissue-specific functions. Combining advances in tissue engineering microfabrication, OoCs have gained interest as a next-generation experimental platform investigate pathophysiology effect of therapeutics body. There many examples there applications, making it difficult for...
Oxygen measurements in different microtissue culture environments were accomplished with the use of phase fluorimetry on dispersible oxygen microsensors.
Abstract Simultaneous detection of multiple analytes from a single sample (multiplexing), particularly when done at the point need, can guide complex decision-making without increasing required volume or cost per test. Despite recent advances, multiplexed analyte sensing still typically faces critical limitation measuring only one type molecule (e.g., small molecules nucleic acids) assay platform. Here, we address this bottleneck with customizable platform that integrates cell-free...
Intestinal organoids are 3D cell structures that replicate some aspects of organ function and organized with a polarized epithelium facing central lumen. To enable more applications, new technologies needed to access the luminal cavity apical surface organoids. We developed perfusion system utilizing double-barrel glass capillary pressure-based pump modify contents human intestinal organoid for extended periods time while applying cyclic cellular strain. Cyclic injection withdrawal...
Abstract Simultaneous detection of multiple analytes from a single sample (multiplexing), particularly when at the point need, can guide complex decision-making without increasing required volume or cost per test. Despite recent advances, multiplexing still typically faces critical limitation measuring only one type molecule assay platform – for example, small molecules nucleic acids. In this work, we address bottleneck with customizable that integrates cell-free expression (CFE)...
<h3>Background</h3> Protein-protein interaction (PPI) is one of the many mechanisms where individual cells understand and modulate surrounding environment through communication with nearby or extracellular matrix. In diseases including cancer, some PPIs have been identified to adversely impact immune response. One well-known example between programmed cell death ligand 1 (PD-L1) protein (PD-1). Tumor utilize PD-1/PD-L1 evade activities. While immunotherapies targeting blockade approved by...
Abstract Glioblastomas are one of the most aggressive forms brain tumors characterized by distinct genetic and molecular signatures. Compared to other solid tumors, role immune cells in progression, invasion prognosis is not well studies for CNS tumors. Here, we demonstrate a novel RNAscope Multiomic Assay that enables detection multiple RNA protein markers simultaneously. With this TSA amplification-based co-detection assay visualized few combinations 3 marker panels on human FFPE normal...
To mimic physiological microenvironments in organ-on-a-chip systems, physiologically relevant parameters are required to precisely access drug metabolism. Oxygen level is a critical microenvironmental parameter maintain cellular or tissue functions and modulate their behaviors. Current setups oftentimes subjected the ambient incubator oxygen at 21%, which higher than most if not all concentrations. Additionally, each different ranging from 0.5 13%. Here, closed-loop modular...
Abstract Understanding tissue heterogeneity is critical for elucidating cell-cell interactions with important implications in immuno-oncology, inflammation, and neuroscience. Tissue poses immense challenges to understanding underlying molecular mechanisms using techniques such as qRT-PCR or bulk sequencing. While single-cell RNA sequencing can provide information about precise cellular composition of tissues, data analysis be cumbersome spatial context lost. With platforms RNAscope, target...
<h3>Background</h3> Spatial biology methods are increasingly used for the characterization of complex tissue microenvironments, understanding which can shed light on fundamental biological mechanisms and better inform development targeted therapeutics. RNAscope™ <i>in situ</i> hybridization (ISH) technology, capable highly sensitive single-molecule RNA detection, be combined with immunohistochemistry (IHC) or immunofluorescence (IF) co-detection clinically relevant biomarkers same slide...
<h3>Background</h3> Studying cell-cell interactions can have important implications in immuno-oncology, inflammation, and neuroscience. Tissue heterogeneity poses immense challenges to understanding underlying molecular mechanisms using techniques such as qRT-PCR or bulk sequencing. While single-cell RNA sequencing provide information about precise cellular composition of tissues, spatial context is lost. With platforms RNAscope, target gene protein expression be visualized characterize cell...
<h3>Background</h3> Tumor cells employ multiple tactics to evade immune cell activities. Programmed death ligand 1 (PD-L1) is one of the key antigens presented on tumor that bind programmed protein (PD-1) and block response. Multiple immunotherapies targeting PD-1/PD-L1 blockade have been approved by FDA. Currently, patients are tested for PD-L1 expression be considered checkpoint inhibitor immunotherapies. Clinical outcomes these immunotherapies, however, do not always correlate level...
Abstract Development of next-generation immunotherapies need a highly sensitive multiomics platform to evaluate diagnostic and therapeutic biomarkers. Tissue heterogeneity poses immense challenges understanding underlying molecular mechanisms using techniques such as qRT-PCR or bulk sequencing. Single-cell RNA sequencing can provide information about precise cellular composition tissues, data analysis be cumbersome spatial context is lost. With single-cell platforms RNAscope, target gene...