A5006435743- 3D Printing in Biomedical Research
- Electrospun Nanofibers in Biomedical Applications
- Tissue Engineering and Regenerative Medicine
- Innovative Microfluidic and Catalytic Techniques Innovation
- Cancer Cells and Metastasis
- Bone Tissue Engineering Materials
- Cystic Fibrosis Research Advances
- Collagen: Extraction and Characterization
- Cancer Diagnosis and Treatment
- Additive Manufacturing and 3D Printing Technologies
- bioluminescence and chemiluminescence research
- Bacterial biofilms and quorum sensing
- Cancer-related Molecular Pathways
- Brain Metastases and Treatment
- Glioma Diagnosis and Treatment
- Escherichia coli research studies
- Microfluidic and Capillary Electrophoresis Applications
- Biomedical and Engineering Education
- Cell death mechanisms and regulation
- Lung Cancer Treatments and Mutations
- Endodontics and Root Canal Treatments
- Cellular Mechanics and Interactions
- FOXO transcription factor regulation
- Polydiacetylene-based materials and applications
- Congenital heart defects research
Dalhousie University
2017-2024
Beatrice Hunter Cancer Research Institute
2022
Vancouver Clinic
2021
University of Michigan
2013-2019
Ann Arbor Center for Independent Living
2014-2019
BioSurfaces (United States)
2014-2017
University of Toronto
2005-2014
Ulsan National Institute of Science and Technology
2012-2013
Rice University
2012
University of Ottawa
2003
Journal Article On being the right size: scaling effects in designing a human-on-a-chip Get access Christopher MoraesEqual contributions., contributions. Department of Biomedical Engineering, College University Michigan, 2200 Bonisteel Blvd, Ann Arbor, MI 48109, USA Search for other works by this author on: Oxford Academic Google Scholar Joseph M. Labuz, Labuz Brendan Leung, Leung Mayumi Inoue, Inoue Internal Medicine, Michigan Health System, 1500 E. Medical Center Drive, Tae-Hwa Chun, Chun...
Nephrotoxicity is often underestimated because renal clearance in animals higher compared to humans. This paper aims illustrate the potential fill such pharmacokinetic gaps between and humans using a microfluidic kidney model. As an initial demonstration, we compare nephrotoxicity of drug, administered at same total dosage, but different regimens. Kidney epithelial cell, cultured under physiological shear stress conditions, are exposed gentamicin regimens that mimic pharmacokinetics bolus...
Three-dimensional spheroid cultures have become increasingly popular as drug screening platforms, especially with the advent of different high throughput forming technologies. However, comparing efficacy across cell types in culture can be difficult due to variations morphologies and transport characteristics. Improving reproducibility compact, circular spheroids contributes standardizing increasing fidelity desired gradient profiles these three-dimensional tissue cultures. In this study we...
Thermodynamics drive crystalline organic molecules to be crystallized at temperatures below their melting point. Even though can form supercooled liquids by rapid cooling, materials readily undergo a phase transformation an energetically favorable upon subsequent heat treatment. Opposite this general observation, here, we report molecular design of thermally stable liquid diketopyrrolopyrrole (DPP) derivatives and intriguing shear-triggered crystallization with dramatic optical property...
Oxygen measurements in different microtissue culture environments were accomplished with the use of phase fluorimetry on dispersible oxygen microsensors.
Brain metastases are the most lethal complication of advanced cancer; therefore, it is critical to identify when a tumor has potential metastasize brain. There currently no interventions that shed light on primary tumors We constructed and tested platform quantitatively profile dynamic phenotypes cancer cells from aggressive triple negative breast cell lines patient derived xenografts (PDXs), generated brain diverse organs origin. Combining an live imaging algorithm artificial intelligence,...
Functional cardiac tissue was prepared using a modular engineering approach with the goal of creating vascularized tissue. Rat aortic endothelial cells (RAEC) were seeded onto submillimeter-sized modules made type I bovine collagen supplemented Matrigel™ (25% v/v) embedded cardiomyocyte (CM)-enriched neonatal rat heart and assembled into contractile, macroporous, sheet-like construct. Modules (without RAEC) cultured in 10% serum (BS) more contractile responsive to external stimulus (lower...
We describe patterning of bacterial biofilms using polymer-based aqueous two-phase system (ATPS) microprinting protocols. The fully but selectively bacteria-partitioning nature the ATPS allows spatially distinct localization suspensions bacteria such as Pseudomonas aeruginosa and Escherichia coli with high precision. patterned form over time. Due to gentle noncontact printing procedures employed, coculture composed multiple types could be printed not only adjacent each other also directly...
Micropatterning of bacteria using aqueous two phase system (ATPS) enables the localized culture and formation physically separated bacterial communities on human epithelial cell sheets. This method was used to compare effects Escherichia coli strain MG1655 an isogenic invasive counterpart that expresses invasin (inv) gene from Yersinia pseudotuberculosis underlying layer. Large portions layer beneath were killed or detached while non-invasive E. had no apparent effect over a 24 h observation...
Patients with many types of malignancy commonly harbor quiescent disseminated tumor cells in bone marrow. These frequently resist chemotherapy and may persist for years before proliferating as recurrent metastases. To test compounds that eliminate cancer cells, we established a new 384-well 3D spheroid model which small numbers reversibly arrest G1/G0 phase the cell cycle when cultured marrow stromal cells. Using dual-color bioluminescence imaging to selectively quantify viability same...
To replace damaged or diseased tissues, large tissue‐engineered constructs can be prepared by assembling modular components in a bottom‐up approach. However, high‐speed method is needed to produce sufficient numbers of these modules for full‐sized tissue substitutes. this end, novel production technique devised, combining air shearing and plug flow reactor‐style design rapidly quantities hydrogel‐based (here type I collagen) cylindrical with tunable diameters length. Using technique,...
Three-dimensional (3D) culture systems such as cell-laden hydrogels are superior to standard two-dimensional (2D) monolayer cultures for many drug-screening applications. However, their adoption into high-throughput screening (HTS) has been lagging, in part because of the difficulty incorporating these formats existing robotic liquid handling and imaging infrastructures. Dispensing prepolymer solutions 2D well plates is a potential solution but typically requires large volumes reagents avoid...
An entirely new approach to tissue engineering is presented that uses the interfacial forces between aqueous solutions of phase‐separating polymers confine cells and promote their assembly into interconnected, macroscopic constructs. This simple inexpensive general procedure creates free‐standing, centimeter‐scale constructs from cell suspensions at interface poly(ethylene glycol) dextran two‐phase systems in as little 2 h. Using this method, skin are produced integrate with decellularized...
The human mucus layer plays a vital role in maintaining health by providing physical barrier to pathogens. This biological hydrogel also provides the microenvironment for commensal bacteria. Common models used study host-microbe interactions include gnotobiotic animals or mammalian-microbial co-culture platforms. Many of current vitro lack sufficient host these interactions. In this study, we engineered mucus-like Consisting mixed alginate-mucin (ALG-MUC) network using low concentration...
The effects of cross-linked poloxamine hydrogels on the cellular function embedded HepG2 cells and surface-attached endothelial were assessed. within collagen/poloxamine-methacrylate gel survived photo-cross-linking (MTT viability, 78%). There was a gradual increase in cell number during first week. cumulative secretion alpha1-antitrypsin by showed an almost linear profile. However, lower levels for matrix observed when compared with collagen. Endothelial attached poorly to gels without...
The airway mucus of patients with cystic fibrosis has altered properties, which create a microenvironment primed for chronic infections that are difficult to treat. These complex polymicrobial and corresponding mammalian-microbe interactions challenging model in vitro. Here, we report the development mucus-like hydrogels varied compositions viscoelastic properties reflecting differences between healthy mucus. Models microenvironments were created by combining relevant pathogens, human...
Abstract Collagen‐containing crosslinked, remodelable poloxamine derivatives were produced by introducing very short oligo(lactic acid) segments through the reaction of with L ‐lactide and later addition unsaturated bonds modified methacryloyl chloride. Degradation studies on discs indicated a faster weight loss in comparison to stability lactoyl‐free samples. Cell‐containing modules (both HepG2 cells two different umbilical vein smooth muscle cell (UVSMC) cell‐types) produced. Live/Dead...