A5022046396- Neuroinflammation and Neurodegeneration Mechanisms
- Neurogenesis and neuroplasticity mechanisms
- Regulation of Appetite and Obesity
- MicroRNA in disease regulation
- Immune cells in cancer
- Neurobiology and Insect Physiology Research
- Single-cell and spatial transcriptomics
- Nuclear Receptors and Signaling
- Neurological disorders and treatments
- Gear and Bearing Dynamics Analysis
- Neuroscience and Neuropharmacology Research
- Cancer Immunotherapy and Biomarkers
- Cancer Cells and Metastasis
- Molecular Biology Techniques and Applications
- Nerve injury and regeneration
- Dementia and Cognitive Impairment Research
- Parkinson's Disease Mechanisms and Treatments
- Cellular Mechanics and Interactions
- Zebrafish Biomedical Research Applications
- Erythrocyte Function and Pathophysiology
- Erythropoietin and Anemia Treatment
- Genetics and Neurodevelopmental Disorders
- Cell Adhesion Molecules Research
- Alzheimer's disease research and treatments
- Autoimmune Neurological Disorders and Treatments
Advanced Cell Diagnostics (United States)
2024
Indian Institute of Science Education and Research Pune
2021-2023
Stanford University
2022
Menlo School
2022
Max Planck Institute of Experimental Medicine
2018-2020
Nanoscale Microscopy and Molecular Physiology of the Brain Cluster of Excellence 171 — DFG Research Center 103
2018
Abstract Erythropoietin (EPO), named after its role in hematopoiesis, is also expressed mammalian brain. In clinical settings, recombinant EPO treatment has revealed a remarkable improvement of cognition, but underlying mechanisms have remained obscure. Here, we show with novel line reporter mice that cognitive challenge induces local/endogenous hypoxia hippocampal pyramidal neurons, hence enhancing expression and receptor (EPOR). High-dose administration, amplifying auto/paracrine EPO/EPOR...
Autoantibodies of the IgG class against N-methyl-D-aspartate-receptor subunit-NR1 (NMDAR1-AB) were considered pathognomonic for anti-NMDAR encephalitis. This view has been challenged by age-dependent seroprevalence (up to >20%) functional NMDAR1-AB all immunoglobulin classes found in >5000 individuals, healthy or affected different diseases. These findings question a merely encephalitogenic role NMDAR1-AB. Here, we show that belong normal autoimmune repertoire dogs, cats, rats, mice,...
Abstract Spatial Multiomics allows for context-based study of the tumor microenvironment (TME). TME consists diverse cells that collectively influence progression, therapeutic response, and immune activation. CD8 T-cells, essential antitumor immunity, often become exhausted in TME. Recent studies show PD1+ TCF1+ stem-like T-cells retain differentiation potential can be rejuvenated through cytokine signaling checkpoint modulation. This detailed spatial profiling RNA protein expression is...
Abstract Advances in our understanding of the tumor microenvironment (TME) will help inform development next generation immunotherapies and engineered immune-cell therapies. Spatially characterizing immune cells with cell marker protein activation RNAs is crucial to determine therapeutic efficacy potential toxicity. To address this, we have developed a high throughput RNAscope™ assay on BOND RX spatially visualize RNA markers same slide. We utilized new Multiomic LS that can detect up 6...
Dynamic reconfiguration of circuit function subserves the flexibility innate behaviors tuned to physiological states. Internal energy stores adaptively regulate feeding-associated and integrate opposing hunger satiety signals at level neural circuits. Across vertebrate lineages, neuropeptides cocaine- amphetamine-regulated transcript (CART) neuropeptide Y (NPY) have potent anorexic orexic functions, respectively, show energy-state-dependent expression in interoceptive neurons. However, how...
<h3>Background</h3> Protein-protein interaction (PPI) is one of the many mechanisms where individual cells understand and modulate surrounding environment through communication with nearby or extracellular matrix. In diseases including cancer, some PPIs have been identified to adversely impact immune response. One well-known example between programmed cell death ligand 1 (PD-L1) protein (PD-1). Tumor utilize PD-1/PD-L1 evade activities. While immunotherapies targeting blockade approved by...
<h3>Background</h3> Tumormicroenvironment (TME) is a complex milieu of multiple cell types mainly including tumor cells, immune endothelial cells and stromal cells. Immune profile within the TME can determine success immunotherapies, indicate therapeutic efficacy as well any potential toxicity. Spatially interrogating these crucial in studying immune-immune immune-tumor interactions. Characterizing require assessing marker expression using protein detection activation markers RNA detection....
Abstract Glioblastomas are one of the most aggressive forms brain tumors characterized by distinct genetic and molecular signatures. Compared to other solid tumors, role immune cells in progression, invasion prognosis is not well studies for CNS tumors. Here, we demonstrate a novel RNAscope Multiomic Assay that enables detection multiple RNA protein markers simultaneously. With this TSA amplification-based co-detection assay visualized few combinations 3 marker panels on human FFPE normal...
ABSTRACT Dynamic re-configuration of circuit function subserves the flexibility innate behaviours tuned to physiological states. Internal energy stores adaptively regulate feeding-associated by integrating opposing hunger and satiety signals at level neural circuits. Across vertebrate lineages, neuropeptides CART NPY have potent anorexic orexic functions, respectively, show state-dependent expression in interoceptive neurons. However, how antagonistic activities these peptides modulate...