A5050114695- Pluripotent Stem Cells Research
- CRISPR and Genetic Engineering
- Neuroscience and Neuropharmacology Research
- Neural dynamics and brain function
- Single-cell and spatial transcriptomics
- Alzheimer's disease research and treatments
- Diet and metabolism studies
- Renal and related cancers
- 3D Printing in Biomedical Research
- Neuroinflammation and Neurodegeneration Mechanisms
- RNA Research and Splicing
- Animal Genetics and Reproduction
- Neurogenesis and neuroplasticity mechanisms
- RNA Interference and Gene Delivery
- DNA and Biological Computing
- Reproductive Biology and Fertility
- Bioinformatics and Genomic Networks
- Neuroscience and Neural Engineering
- Tissue Engineering and Regenerative Medicine
- Hair Growth and Disorders
- Immune Cell Function and Interaction
- Cancer-related molecular mechanisms research
- Memory and Neural Mechanisms
- Cell Image Analysis Techniques
University of California, Los Angeles
2017-2022
Broad Center
2019-2021
University of Sheffield
2015-2018
Genetic changes in human pluripotent stem cells (hPSCs) gained during culture can confound experimental results and potentially jeopardize the outcome of clinical therapies. Particularly common hPSCs are trisomies chromosomes 1, 12, 17, 20. Thus, should be regularly screened for such aberrations. Although a number methods used to assess hPSC genotypes, there has been no systematic evaluation sensitivity commonly techniques detecting low-level mosaicism cultures. We have performed mixing...
Abstract The International Stem Cell Initiative compared several commonly used approaches to assess human pluripotent stem cells (PSC). PluriTest predicts pluripotency through bioinformatic analysis of the transcriptomes undifferentiated cells, whereas, embryoid body (EB) formation in vitro and teratoma vivo provide direct tests differentiation. Here we report that EB assays, analyzed after differentiation under neutral conditions promoting ectoderm, mesoderm, or endoderm lineages, are...
Abstract Aggregation of hyperphosphorylated tau in neurofibrillary tangles (NFTs) is closely associated with neuronal death and cognitive decline Alzheimer’s disease (AD). To define the signatures that distinguish between aggregation-prone resistant cell states AD, we developed a FACS-based method for high-throughput isolation transcriptome profiling individual cells cytoplasmic aggregates profiled 63,110 somas from human AD brains. By comparing NFT-bearing NFT-free within across subtypes,...
Human embryonic stem cells (hESCs) display substantial heterogeneity in gene expression, implying the existence of discrete substates within cell compartment. To determine whether these impact fate decisions hESCs we used a GFP reporter line to investigate properties fractions putative undifferentiated defined by their differential expression endoderm transcription factor, GATA6, together with hESC surface marker, SSEA3. By single-cell cloning, confirmed that characterized GATA6 and SSEA3...
The specification of inhibitory neurons has been described for the mouse and human brain, many studies have shown that pluripotent stem cells (PSCs) can be used to create interneurons in vitro. It is unclear whether vitro methods produce generate all subtypes found how similar vivo are. We applied single-nuclei single-cell transcriptomics model interneuron development from cortex derived PSCs. provide a direct comparison various derivation determine homogeneity achieved. find PSC-derived...
ABSTRACT Brain organoids represent a powerful tool for the study of human neurological diseases, particularly those impacting brain growth and structure. However, many diseases manifest with clear evidence physiological network abnormality in absence anatomical changes. This raises question whether possess sufficient neural complexity to model these conditions. Here, we explore level functions using calcium sensor imaging extracellular recording approaches that together reveal existence...
Maternal exposure during pregnancy to toxins can occasionally lead miscarriage and malformation. It is currently thought that pass through the placental barrier, albeit bi-layered in first trimester, damage fetus directly, at low concentration. Here we examined responses of human embryonic stem (hES) cells tissue culture two metals We compared direct exposures with indirect across a model placenta cell barrier. Direct caused increased DNA without apoptosis or loss number but some evidence...
Abstract: A significant bottleneck in drug discovery is the lack of suitable models for sensitive, reliable, and rapid assessment lead molecules preclinical stages discovery. Human pluripotent stem cells (hPSCs) derived either from early human blastocysts (human embryonic cells) or by reprogramming somatic to a state (human-induced can be propagated extensively vitro while retaining ability differentiate into any specialized cell type within body. In this review, we discuss how these unique...