A5062417486- Single-cell and spatial transcriptomics
- Context-Aware Activity Recognition Systems
- RNA Research and Splicing
- Renal and related cancers
- Pluripotent Stem Cells Research
- Opportunistic and Delay-Tolerant Networks
- CRISPR and Genetic Engineering
- Cancer Genomics and Diagnostics
- Emotion and Mood Recognition
- Cancer-related molecular mechanisms research
- Neurogenesis and neuroplasticity mechanisms
- Biliary and Gastrointestinal Fistulas
- Methemoglobinemia and Tumor Lysis Syndrome
- Academic Writing and Publishing
- Bluetooth and Wireless Communication Technologies
- Groundwater and Isotope Geochemistry
- Genetic Syndromes and Imprinting
- IoT and Edge/Fog Computing
- Peer-to-Peer Network Technologies
- Congenital heart defects research
- Genetic and Clinical Aspects of Sex Determination and Chromosomal Abnormalities
- Inflammatory Myopathies and Dermatomyositis
- Fluoride Effects and Removal
- Skin and Cellular Biology Research
- Skin Diseases and Diabetes
Chinese Academy of Sciences
2024
Research Center for Eco-Environmental Sciences
2024
University of Pittsburgh
2023
Agency for Science, Technology and Research
2015-2023
Institute of Molecular and Cell Biology
2023
Harvard University
2017-2018
Genome Institute of Singapore
2015-2018
University of Oxford
2015-2018
European Bioinformatics Institute
2016-2018
The Francis Crick Institute
2013-2017
Here we provide fundamental insights into early human development by single-cell RNA-sequencing of and mouse preimplantation embryos. We elucidate conserved transcriptional programs along with those that are human-specific. Importantly, validate our findings at the protein level, which further reveals differences in embryo gene expression. For example, identify several genes exclusively expressed pluripotent epiblast including transcription factor KLF17. Key components TGF-β signaling...
GABAergic interneurons (GABA, γ-aminobutyric acid) regulate neural-circuit activity in the mammalian cerebral cortex. These cortical are structurally and functionally diverse. Here, we use single-cell transcriptomics to study origins of this diversity mouse. We identify distinct types progenitor cells newborn neurons ganglionic eminences, embryonic proliferative regions that give rise interneurons. precursors show temporally spatially restricted transcriptional patterns lead different...
Abstract The International Stem Cell Initiative compared several commonly used approaches to assess human pluripotent stem cells (PSC). PluriTest predicts pluripotency through bioinformatic analysis of the transcriptomes undifferentiated cells, whereas, embryoid body (EB) formation in vitro and teratoma vivo provide direct tests differentiation. Here we report that EB assays, analyzed after differentiation under neutral conditions promoting ectoderm, mesoderm, or endoderm lineages, are...
Pax1 and Pax9 play redundant, synergistic functions in the patterning differentiation of sclerotomal cells that give rise to vertebral bodies intervertebral discs (IVD) axial skeleton. They are conserved mice humans, whereby mutations/deficiency human PAX1/PAX9 have been associated with kyphoscoliosis. By combining cell-type specific transcriptome ChIP-sequencing data, we identified roles Pax1/Pax9 cell proliferation, cartilage development collagen fibrillogenesis, which vital early IVD...
The RNA-binding protein SRSF3 (also known as SRp20) has critical roles in the regulation of pre-mRNA splicing. Zygotic knockout
Transplantation of stem cell–derived retinal pigment epithelial (RPE) cells is considered a viable therapeutic option for age-related macular degeneration (AMD). Several landmark Phase I/II clinical trials have demonstrated safety and tolerability RPE transplants in AMD patients, albeit with limited efficacy. Currently, there understanding how the recipient retina regulates survival, maturation, fate specification transplanted cells. To address this, we into subretinal space immunocompetent...
Accurately characterizing transcription factor (TF)-DNA affinity is a central goal of regulatory genomics. Although thermodynamics provides the most natural language for describing continuous range TF-DNA affinity, traditional motif discovery algorithms focus instead on classification paradigms that aim to discriminate 'bound' and 'unbound' sequences. Moreover, these do not directly model distribution tags in ChIP-seq data. Here, we present new algorithm named T hermodynamic M odeling ChIP-...
Embryogenesis is an intricate process involving multiple genes and pathways. Some of the key transcription factors controlling specific cell types are Sox trio, namely, Sox5 , Sox6 Sox9 which play crucial roles in organogenesis working a concerted manner. Much however still needs to be learned about their combinatorial during this process. A developmental genomics systems biology approach offers complement reductionist methodology current provide more comprehensive integrated view...
Rational: Nodular cutaneous lupus mucinosis is regarded as a distinctive deposition with systemic erythematosus(SLE). All typical cases occurred asymptomatic papules, nodules, or plaques on the trunk, upper and lower extremities, face. Histopathology mainly revealed abundant mucin deposits among splayed collagen bundles in dermis. At same time we can find A clinical manifestations biological evidence of SLE. Here, report first case nodular that did not present any prior symptoms history...
Dubin-Johnson syndrome is a rare, autosomal recessive hereditary disease, commonly known as "black liver disease." It of great interest for surgeons to accidentally find the liver" during laparoscopic cholecystectomy (LC). We performed an emergency LC cholecystolithiasis with in 2013. only and tissue biopsy. Following 5-year follow-up period, patient does not appear have abdominal pain any other discomfort. has no significant relationship occurrence generally requires special treatment....
Objectives: To study the incidence of Down's syndrome by maternal age Asian population. Setting and Design: Data on prenatal serum screening between 2008-2017 was obtained from Maternal Child Hospital Hubei Province. Cases were classified into three groups: ≤26-year-old, 27-33-year-old, ≥34-year-old. The prevalence Syndrome calculated for each bracket separately, difference different groups analyzed with chi-square test. Results: Down’s in ≥34-year-old 0.67 ‰, 0.29 2.07 respectively. There...