A5006095435- Pluripotent Stem Cells Research
- Neuroinflammation and Neurodegeneration Mechanisms
- Tryptophan and brain disorders
- Renal and related cancers
- Mosquito-borne diseases and control
- Glioma Diagnosis and Treatment
- Neurogenesis and neuroplasticity mechanisms
- Neural dynamics and brain function
- Epigenetics and DNA Methylation
- Diet and metabolism studies
- Neuroscience and Neuropharmacology Research
- Cancer, Hypoxia, and Metabolism
- Memory and Neural Mechanisms
- Neuroscience and Neural Engineering
- Cell Image Analysis Techniques
- Malaria Research and Control
- Gut microbiota and health
- Kruppel-like factors research
- HIV Research and Treatment
- Cancer-related molecular mechanisms research
- RNA Research and Splicing
- MicroRNA in disease regulation
Broad Center
2017-2024
University of California, Los Angeles
2017-2022
Intel (United States)
2022
Center for Autism and Related Disorders
2020
Telencephalic organoids generated from human pluripotent stem cells (hPSCs) are a promising system for studying the distinct features of developing brain and underlying causes many neurological disorders. While organoid technology is steadily advancing, challenges remain, including potential batch-to-batch cell-line-to-cell-line variability, structural inconsistency. Here, we demonstrate that major contributor to cortical quality way hPSCs maintained prior differentiation. Optimal results...
ABSTRACT Brain organoids represent a powerful tool for the study of human neurological diseases, particularly those impacting brain growth and structure. However, many diseases manifest with clear evidence physiological network abnormality in absence anatomical changes. This raises question whether possess sufficient neural complexity to model these conditions. Here, we explore level functions using calcium sensor imaging extracellular recording approaches that together reveal existence...
Abstract Neural progenitor cells within the cerebral cortex undergo a characteristic switch between symmetric self-renewing cell divisions early in development and asymmetric neurogenic later. Yet, mechanisms controlling this transition remain unclear. Previous work has shown that but not late neural (NPCs) endogenously express autism-linked transcription factor Foxp1, both loss gain of Foxp1 function can alter NPC activity fate choices. Here, we show premature upregulates transcriptional...
SUMMARY Telencephalic organoids generated from human pluripotent stem cells (hPSCs) are emerging as an effective system to study the distinct features of developing brain and underlying causes many neurological disorders. While progress in organoid technology has been steadily advancing, challenges remain including rampant batch-to-batch cell line-to-cell line variability irreproducibility. Here, we demonstrate that a major contributor successful cortical production is manner which hPSCs...
Protocol to differentiate neural progenitor cells mature neurons and astrocytes in 2D culture.
Abstract Background Maternal immune activation (MIA) is a proposed risk factor for multiple neurodevelopmental and psychiatric disorders, including schizophrenia. However, the molecular neurobiological mechanisms through which MIA imparts these disorders remain poorly understood. A recently developed nonhuman primate model of exposure to viral mimic poly:ICLC during pregnancy shows abnormal social repetitive behaviors elevated striatal dopamine, hallmark human psychosis, providing an...
ABSTRACT Radial glia progenitors within the cerebral cortex undergo a characteristic switch between symmetric self-renewing cell divisions early in development and asymmetric neurogenic at later times, yet mechanisms controlling this transition remain unclear. Previous work has shown that autism-linked transcription factor Foxp1 is endogenously expressed by but not late radial glia, both loss gain of can alter their neural progenitor activities fate choices. Here, we show premature leads to...
Radial glia progenitors within the cerebral cortex undergo a characteristic switch between symmetric self-renewing cell divisions early in development and asymmetric neurogenic at later times, yet mechanisms controlling this transition remain unclear. Previous work has shown that autism-linked transcription factor Foxp1 is endogenously expressed by but not late radial glia, both loss gain of can alter their neural progenitor activities fate choices. Here, we show premature leads to an...